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They are currently exploited in several nanomedicine formulations. However, their development and application is still limited by expensive and time-consuming process development and production methods. Therefore, to exploit these systems more effectively and support the rapid translation of new liposomal nanomedicines from bench to bedside, new cost-effective and scalable production methods are needed. We present a continuous process flow system for the preparation, modification and purification of liposomes which offers lab-on-chip scale production. The system was evaluated for a range of small vesicles (below 300\u2009nm) varying in lipid composition, size and charge; it offers effective and rapid nanomedicine purification with high lipid recovery (&gt;\u200998%) combined with effective removal of non-entrapped drug (propofol &gt;95% reduction of non-entrapped drug present) or protein (ovalbumin &gt;90% reduction of OVA present) and organic solvent (ethanol &gt;95% reduction) in less than 4\u2009minutes. The key advantages of using this bench-top, rapid, process development tool are the flexible operating conditions, interchangeable membranes and scalable high-throughput yields, thereby offering simultaneous manufacturing and purification of nanoparticles with tailored surface attributes.<\/jats:p>","DOI":"10.1038\/s41598-017-11533-1","type":"journal-article","created":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T16:44:44Z","timestamp":1505407484000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":170,"title":["Formation and purification of tailored liposomes for drug delivery using a module-based micro continuous-flow system"],"prefix":"10.1038","volume":"7","author":[{"given":"Nikolay","family":"Dimov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Kastner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Hussain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Perrie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6204-5074","authenticated-orcid":false,"given":"Nicolas","family":"Szita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,9,21]]},"reference":[{"issue":"25876","key":"11533_CR1","doi-asserted-by":"publisher","DOI":"10.1038\/srep25876","volume":"6","author":"D Carugo","year":"2016","unstructured":"Carugo, D., Bottaro, E., Owen, J., Stride, E. & Nastruzzi, C. 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