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In the present study, we have synthesized nanoparticles of Amphotericin B (AmB), a potent antifungal agent, using <jats:italic>Aloe vera<\/jats:italic> leaf extract. The synthesis of AmB nano-assemblies (AmB-NAs) was established employing spectro-photometric and electron microscopic studies, while their crystalline nature was established by X-ray diffraction. AmB-nano-formulation showed much higher stability in both phosphate buffer saline and serum and exhibit sustained release of parent drug over an extended time period. The as-synthesized AmB-NA possessed significantly less haemolysis as well as nephrotoxicity in the host at par with Ambisome<jats:sup>\u00ae<\/jats:sup>, a liposomized AmB formulation. Interestingly, the AmB-NAs were more effective in killing various fungal pathogens including <jats:italic>Candida<\/jats:italic> spp. and evoked less drug related toxic manifestations in the host as compared to free form of the drug. The data of the present study suggest that biomimetically synthesized AmB-NA circumvent toxicity issues and offer a promising approach to eliminate systemic fungal infections in Balb\/C mice.<\/jats:p>","DOI":"10.1038\/s41598-017-11847-0","type":"journal-article","created":{"date-parts":[[2017,9,13]],"date-time":"2017-09-13T11:18:04Z","timestamp":1505301484000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Biomimetically engineered Amphotericin B nano-aggregates circumvent toxicity constraints and treat systemic fungal infection in experimental animals"],"prefix":"10.1038","volume":"7","author":[{"given":"Qamar","family":"Zia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Owais","family":"Mohammad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd Ahmar","family":"Rauf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wasi","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swaleha","family":"Zubair","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,9,19]]},"reference":[{"key":"11847_CR1","first-page":"197","volume":"119","author":"SW Chapman","year":"2008","unstructured":"Chapman, S. 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