{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T11:40:47Z","timestamp":1788954047082,"version":"build-2803163510"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,10,18]],"date-time":"2017-10-18T00:00:00Z","timestamp":1508284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,10,18]],"date-time":"2017-10-18T00:00:00Z","timestamp":1508284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The recent outbreak of Zika virus (ZIKV) disease caused an enormous number of infections in Central and South America, and the unusual increase in the number of infants born with microcephaly associated with ZIKV infection aroused global concern. Here, we developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay using a portable device for the detection of ZIKV. The assay specifically detected ZIKV strains of both Asian and African genotypes without cross-reactivity with other arboviruses, including Dengue and Chikungunya viruses. The assay detected viral RNA at 14.5 TCID<jats:sub>50<\/jats:sub>\/mL in virus-spiked serum or urine samples within 15\u2009min, although it was slightly less sensitive than reference real time RT-PCR assay. We then evaluated the utility of this assay as a molecular diagnostic test using 90 plasma or serum samples and 99 urine samples collected from 120 suspected cases of arbovirus infection in the states of Para\u00edba and Pernambuco, Brazil in 2016. The results of this assay were consistent with those of the reference RT-PCR test. This portable RT-LAMP assay was highly specific for ZIKV, and enable rapid diagnosis of the virus infection. Our results provide new insights into ZIKV molecular diagnostics and may improve preparedness for future outbreaks.<\/jats:p>","DOI":"10.1038\/s41598-017-13836-9","type":"journal-article","created":{"date-parts":[[2017,10,12]],"date-time":"2017-10-12T15:21:09Z","timestamp":1507821669000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Development and evaluation of a rapid molecular diagnostic test for Zika virus infection by reverse transcription loop-mediated isothermal amplification"],"prefix":"10.1038","volume":"7","author":[{"given":"Yohei","family":"Kurosaki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danyelly Bruneska Gondim","family":"Martins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayuko","family":"Kimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andriu dos Santos","family":"Catena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Am\u00e9lia Carlos Souto Maior","family":"Borba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra da Silva","family":"Mattos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruka","family":"Abe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rokusuke","family":"Yoshikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Luiz","family":"de Lima Filho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiro","family":"Yasuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,10,18]]},"reference":[{"key":"13836_CR1","doi-asserted-by":"publisher","first-page":"1552","DOI":"10.1056\/NEJMra1602113","volume":"374","author":"LR Petersen","year":"2016","unstructured":"Petersen, L. R., Jamieson, D. J., Powers, A. M. & Honein, M. A. Zika Virus. N Engl J Med \n                           374, 1552\u20131563 (2016).","journal-title":"N Engl J Med"},{"key":"13836_CR2","doi-asserted-by":"publisher","first-page":"e119","DOI":"10.1016\/S1473-3099(16)30010-X","volume":"16","author":"N Wikan","year":"2016","unstructured":"Wikan, N. & Smith, D. R. Zika virus: history of a newly emerging arbovirus. Lancet Infect Dis \n                           16, e119\u2013126 (2016).","journal-title":"Lancet Infect Dis"},{"key":"13836_CR3","doi-asserted-by":"publisher","first-page":"1885","DOI":"10.3201\/eid2110.150847","volume":"21","author":"GS Campos","year":"2015","unstructured":"Campos, G. S., Bandeira, A. C. & Sardi, S. I. Zika Virus Outbreak, Bahia, Brazil. Emerg Infect Dis \n                           21, 1885\u20131886 (2015).","journal-title":"Emerg Infect Dis"},{"key":"13836_CR4","doi-asserted-by":"publisher","first-page":"951","DOI":"10.1056\/NEJMoa1600651","volume":"374","author":"J Mlakar","year":"2016","unstructured":"Mlakar, J. et al. Zika Virus Associated with Microcephaly. N Engl J Med \n                           374, 951\u2013958 (2016).","journal-title":"N Engl J Med"},{"key":"13836_CR5","doi-asserted-by":"publisher","first-page":"59","DOI":"10.15585\/mmwr.mm6503e2","volume":"65","author":"L Schuler-Faccini","year":"2016","unstructured":"Schuler-Faccini, L. et al. Possible Association Between Zika Virus Infection and Microcephaly - Brazil, 2015. MMWR Morb Mortal Wkly Rep \n                           65, 59\u201362 (2016).","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"13836_CR6","doi-asserted-by":"publisher","first-page":"1356","DOI":"10.1016\/S1473-3099(16)30318-8","volume":"16","author":"TV de Araujo","year":"2016","unstructured":"de Araujo, T. V. et al. Association between Zika virus infection and microcephaly in Brazil, January to May, 2016: preliminary report of a case-control study. Lancet Infect Dis \n                           16, 1356\u20131363 (2016).","journal-title":"Lancet Infect Dis"},{"key":"13836_CR7","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1016\/S0140-6736(16)30902-3","volume":"388","author":"GV Franca","year":"2016","unstructured":"Franca, G. V. et al. Congenital Zika virus syndrome in Brazil: a case series of the first 1501 livebirths with complete investigation. Lancet \n                           388, 891\u2013897 (2016).","journal-title":"Lancet"},{"key":"13836_CR8","doi-asserted-by":"publisher","first-page":"1684","DOI":"10.1111\/trf.13681","volume":"56","author":"ML Barjas-Castro","year":"2016","unstructured":"Barjas-Castro, M. L. et al. Probable transfusion-transmitted Zika virus in Brazil. Transfusion \n                           56, 1684\u20131688 (2016).","journal-title":"Transfusion"},{"key":"13836_CR9","doi-asserted-by":"publisher","first-page":"215","DOI":"10.15585\/mmwr.mm6508e2","volume":"65","author":"SL Hills","year":"2016","unstructured":"Hills, S. L. et al. Transmission of Zika Virus Through Sexual Contact with Travelers to Areas of Ongoing Transmission - Continental United States, 2016. MMWR Morb Mortal Wkly Rep \n                           65, 215\u2013216 (2016).","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"13836_CR10","doi-asserted-by":"crossref","unstructured":"Moreira, J., Peixoto, T. M., Machado de Siqueira, A. & Lamas, C. C. Sexually acquired Zika virus: a systematic review. Clin Microbiol Infect (2017).","DOI":"10.1016\/j.cmi.2016.12.027"},{"key":"13836_CR11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002636","volume":"8","author":"O Faye","year":"2014","unstructured":"Faye, O. et al. Molecular evolution of Zika virus during its emergence in the 20(th) century. PLoS Negl Trop Dis \n                           8, e2636 (2014).","journal-title":"PLoS Negl Trop Dis"},{"key":"13836_CR12","doi-asserted-by":"publisher","DOI":"10.3201\/eid2110.151125","volume":"21","author":"D Musso","year":"2015","unstructured":"Musso, D. Zika Virus Transmission from French Polynesia to Brazil. Emerg Infect Dis \n                           21, 1887 (2015).","journal-title":"Emerg Infect Dis"},{"key":"13836_CR13","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1126\/science.aaf5036","volume":"352","author":"NR Faria","year":"2016","unstructured":"Faria, N. R. et al. Zika virus in the Americas: Early epidemiological and genetic findings. Science \n                           352, 345\u2013349 (2016).","journal-title":"Science"},{"key":"13836_CR14","doi-asserted-by":"publisher","first-page":"84","DOI":"10.3201\/eid2101.140894","volume":"21","author":"AC Gourinat","year":"2015","unstructured":"Gourinat, A. C., O\u2019Connor, O., Calvez, E., Goarant, C. & Dupont-Rouzeyrol, M. Detection of Zika virus in urine. Emerg Infect Dis \n                           21, 84\u201386 (2015).","journal-title":"Emerg Infect Dis"},{"key":"13836_CR15","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.jcv.2015.04.021","volume":"68","author":"D Musso","year":"2015","unstructured":"Musso, D. et al. Detection of Zika virus in saliva. J Clin Virol \n                           68, 53\u201355 (2015).","journal-title":"J Clin Virol"},{"key":"13836_CR16","doi-asserted-by":"publisher","first-page":"475","DOI":"10.15585\/mmwr.mm6518e2","volume":"65","author":"AM Bingham","year":"2016","unstructured":"Bingham, A. M. et al. Comparison of Test Results for Zika Virus RNA in Urine, Serum, and Saliva Specimens from Persons with Travel-Associated Zika Virus Disease - Florida, 2016. MMWR Morb Mortal Wkly Rep \n                           65, 475\u2013478 (2016).","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"13836_CR17","unstructured":"Paz-Bailey, G. et al. Persistence of Zika Virus in Body Fluids - Preliminary Report. N Engl J Med (2017)."},{"key":"13836_CR18","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1128\/JCM.01232-16","volume":"55","author":"K St George","year":"2017","unstructured":"St George, K. et al. Zika Virus Testing Considerations: Lessons Learned from the First 80 Real-Time Reverse Transcription-PCR-Positive Cases Diagnosed in New York State. J Clin Microbiol \n                           55, 535\u2013544 (2017).","journal-title":"J Clin Microbiol"},{"key":"13836_CR19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jcv.2016.06.011","volume":"82","author":"C Fourcade","year":"2016","unstructured":"Fourcade, C. et al. Viral load kinetics of Zika virus in plasma, urine and saliva in a couple returning from Martinique, French West Indies. J Clin Virol \n                           82, 1\u20134 (2016).","journal-title":"J Clin Virol"},{"key":"13836_CR20","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-10-311","volume":"10","author":"O Faye","year":"2013","unstructured":"Faye, O. et al. Quantitative real-time PCR detection of Zika virus and evaluation with field-caught mosquitoes. Virol J \n                           10, 311 (2013).","journal-title":"Virol J"},{"key":"13836_CR21","doi-asserted-by":"publisher","first-page":"1232","DOI":"10.3201\/eid1408.080287","volume":"14","author":"RS Lanciotti","year":"2008","unstructured":"Lanciotti, R. S. et al. Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007. Emerg Infect Dis \n                           14, 1232\u20131239 (2008).","journal-title":"Emerg Infect Dis"},{"key":"13836_CR22","doi-asserted-by":"publisher","first-page":"860","DOI":"10.1128\/JCM.00279-16","volume":"54","author":"JJ Waggoner","year":"2016","unstructured":"Waggoner, J. J. & Pinsky, B. A. Zika Virus: Diagnostics for an Emerging Pandemic Threat. J Clin Microbiol \n                           54, 860\u2013867 (2016).","journal-title":"J Clin Microbiol"},{"key":"13836_CR23","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.jcv.2016.02.009","volume":"77","author":"M Campos Rde","year":"2016","unstructured":"Campos Rde, M. et al. Prolonged detection of Zika virus RNA in urine samples during the ongoing Zika virus epidemic in Brazil. J Clin Virol \n                           77, 69\u201370 (2016).","journal-title":"J Clin Virol"},{"key":"13836_CR24","doi-asserted-by":"crossref","unstructured":"Abd El Wahed, A. et al. Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay. PLoS Curr \n                           9 (2017).","DOI":"10.1371\/currents.outbreaks.a7f1db2c7d66c3fc0ea0a774305d319e"},{"key":"13836_CR25","doi-asserted-by":"crossref","unstructured":"Tappe, D. et al. First case of laboratory-confirmed Zika virus infection imported into Europe, November 2013. Euro Surveill \n                           19 (2014).","DOI":"10.2807\/1560-7917.ES2014.19.4.20685"},{"key":"13836_CR26","doi-asserted-by":"publisher","first-page":"1477","DOI":"10.1002\/jmv.24789","volume":"89","author":"AC Felix","year":"2017","unstructured":"Felix, A. C. et al. Cross reactivity of commercial anti-dengue immunoassays in patients with acute Zika virus infection. J Med Virol \n                           89, 1477\u20131479 (2017).","journal-title":"J Med Virol"},{"key":"13836_CR27","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63","volume":"28","author":"T Notomi","year":"2000","unstructured":"Notomi, T. et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res \n                           28, E63 (2000).","journal-title":"Nucleic Acids Res"},{"key":"13836_CR28","doi-asserted-by":"publisher","first-page":"1956","DOI":"10.1128\/JCM.42.5.1956-1961.2004","volume":"42","author":"TC Hong","year":"2004","unstructured":"Hong, T. C. et al. Development and evaluation of a novel loop-mediated isothermal amplification method for rapid detection of severe acute respiratory syndrome coronavirus. J Clin Microbiol \n                           42, 1956\u20131961 (2004).","journal-title":"J Clin Microbiol"},{"key":"13836_CR29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s12275-015-4656-9","volume":"53","author":"T Notomi","year":"2015","unstructured":"Notomi, T., Mori, Y., Tomita, N. & Kanda, H. Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects. J Microbiol \n                           53, 1\u20135 (2015).","journal-title":"J Microbiol"},{"key":"13836_CR30","doi-asserted-by":"publisher","first-page":"S229","DOI":"10.1093\/infdis\/jiw255","volume":"214","author":"Y Kurosaki","year":"2016","unstructured":"Kurosaki, Y. et al. Deployment of a Reverse Transcription Loop-Mediated Isothermal Amplification Test for Ebola Virus Surveillance in Remote Areas in Guinea. J Infect Dis \n                           214, S229\u2013S233 (2016).","journal-title":"J Infect Dis"},{"key":"13836_CR31","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004472","volume":"10","author":"Y Kurosaki","year":"2016","unstructured":"Kurosaki, Y. et al. Development and Evaluation of Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) Assay Coupled with a Portable Device for Rapid Diagnosis of Ebola Virus Disease in Guinea. PLoS Negl Trop Dis \n                           10, e0004472 (2016).","journal-title":"PLoS Negl Trop Dis"},{"key":"13836_CR32","doi-asserted-by":"crossref","unstructured":"Yun, S. I. et al. Complete Genome Sequences of Three Historically Important, Spatiotemporally Distinct, and Genetically Divergent Strains of Zika Virus: MR-766, P6-740, and PRVABC-59. Genome Announc \n                           4 (2016).","DOI":"10.1128\/genomeA.00800-16"},{"key":"13836_CR33","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1016\/j.jviromet.2016.10.010","volume":"238","author":"X Wang","year":"2016","unstructured":"Wang, X. et al. Rapid and sensitive detection of Zika virus by reverse transcription loop-mediated isothermal amplification. J Virol Methods \n                           238, 86\u201393 (2016).","journal-title":"J Virol Methods"},{"key":"13836_CR34","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1016\/j.bios.2016.06.085","volume":"86","author":"B Tian","year":"2016","unstructured":"Tian, B. et al. Attomolar Zika virus oligonucleotide detection based on loop-mediated isothermal amplification and AC susceptometry. Biosens Bioelectron \n                           86, 420\u2013425 (2016).","journal-title":"Biosens Bioelectron"},{"key":"13836_CR35","doi-asserted-by":"publisher","first-page":"7289","DOI":"10.1021\/acs.analchem.6b01632","volume":"88","author":"J Song","year":"2016","unstructured":"Song, J. et al. Instrument-Free Point-of-Care Molecular Detection of Zika Virus. Anal Chem \n                           88, 7289\u20137294 (2016).","journal-title":"Anal Chem"},{"key":"13836_CR36","doi-asserted-by":"publisher","first-page":"12272","DOI":"10.1021\/acs.analchem.6b03460","volume":"88","author":"D Lee","year":"2016","unstructured":"Lee, D. et al. Simple and Highly Sensitive Molecular Diagnosis of Zika Virus by Lateral Flow Assays. Anal Chem \n                           88, 12272\u201312278 (2016).","journal-title":"Anal Chem"},{"key":"13836_CR37","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000003201","volume":"95","author":"R Pessoa","year":"2016","unstructured":"Pessoa, R. et al. Investigation Into an Outbreak of Dengue-like Illness in Pernambuco, Brazil, Revealed a Cocirculation of Zika, Chikungunya, and Dengue Virus Type 1. Medicine (Baltimore) \n                           95, e3201 (2016).","journal-title":"Medicine (Baltimore)"},{"key":"13836_CR38","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.jcv.2014.12.005","volume":"63","author":"L Zammarchi","year":"2015","unstructured":"Zammarchi, L. et al. Zika virus infections imported to Italy: clinical, immunological and virological findings, and public health implications. J Clin Virol \n                           63, 32\u201335 (2015).","journal-title":"J Clin Virol"},{"key":"13836_CR39","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/j.ijid.2016.11.420","volume":"54","author":"V Duong","year":"2017","unstructured":"Duong, V., Dussart, P. & Buchy, P. Zika virus in Asia. Int J Infect Dis \n                           54, 121\u2013128 (2017).","journal-title":"Int J Infect Dis"},{"key":"13836_CR40","unstructured":"Pan American Health Organization and World Health Organization. Zika \u2013 Epidemiological Update 10 March. http:\/\/www2.paho.org\/hq\/index.php?option=com_content&view=article&id=11599&Itemid=41691&lang=en (2016)."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-13836-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-13836-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-13836-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T12:07:16Z","timestamp":1671797236000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-13836-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,18]]},"references-count":40,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["13836"],"URL":"https:\/\/doi.org\/10.1038\/s41598-017-13836-9","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10,18]]},"assertion":[{"value":"9 May 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 October 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 October 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"13503"}}