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A total of 340 newly reported HIV-infected MSM were recruited proportionally from ten prefectures across Zhejiang province between January and December in 2013. Partial pol gene was amplified and sequenced. Phylogenetic relationship, transmission network and genotypic drug resistance analyses were performed on 311 sequences. HIV-1 subtypes including CRF01_AE (55.9%), CRF07_BC (37.6%), subtype B (1.9%), CRF55_01B (1.3%), CRF68_01B (0.3%), CRF08_BC (0.3%) and URFs (2.6%) were identified. A higher proportion of CRF07_BC and other subtypes existed in the &gt;35 years group, while a higher proportion of CRF01_AE was present in the young group (&lt;35 years). Low prevalence of transmitted drug resistance was found (3.9%, 12\/311). Strains with Hangzhou imprints were diffused across the full phylogenetic tree. Moreover, Hangzhou represented the dominant proportion of local HIV transmission (72.0%) and cross-regional transmission (62.4%) based on the provincial transmission network and possessed the largest number of nodes with \u226550 edges, accounting for 50.0% (10\/20). The complexity of HIV subtypes and an intertwined network was noticed in MSM in Zhejiang province. Hangzhou likely plays a central regional role in the intra-provincial spread of HIV.<\/jats:p>","DOI":"10.1038\/s41598-017-14108-2","type":"journal-article","created":{"date-parts":[[2017,10,18]],"date-time":"2017-10-18T10:33:32Z","timestamp":1508322812000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Highlighting the crucial role of Hangzhou in HIV-1 transmission among men who have sex with men in Zhejiang, China"],"prefix":"10.1038","volume":"7","author":[{"given":"Jiafeng","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihong","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiezhe","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobei","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianmin","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,10,24]]},"reference":[{"key":"14108_CR1","unstructured":"National Health and Family Planning Commission of the People\u2019s Republic of China. 2015 China AIDS Response Progress Report, http:\/\/www.unaids.org\/sites\/default\/files\/country\/documents\/CHN_narrative_report_2015.pdf (2015)."},{"key":"14108_CR2","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1093\/cid\/cit210","volume":"57","author":"Z Wu","year":"2013","unstructured":"Wu, Z. et al. 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