{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T02:57:49Z","timestamp":1789095469172,"version":"build-2803163510"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,11,15]],"date-time":"2017-11-15T00:00:00Z","timestamp":1510704000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,11,15]],"date-time":"2017-11-15T00:00:00Z","timestamp":1510704000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CDC73\/Parafibromin is a critical component of the Paf1 complex (PAF1C), which is involved in transcriptional elongation and histone modifications. Mutations of the human <jats:italic>CDC73\/HRPT2<\/jats:italic> gene are associated with hyperparathyroidism-jaw tumor (HPT-JT) syndrome, an autosomal dominant disorder. CDC73\/parafibromin was initially recognized as a tumor suppressor by inhibiting cell proliferation via repression of <jats:italic>cyclin D1<\/jats:italic> and <jats:italic>c-myc<\/jats:italic> genes. In recent years, it has also shown oncogenic features by activating the canonical Wnt\/\u03b2-catenin signal pathway. Here, through limited proteolysis analysis, we demonstrate that the evolutionarily conserved human CDC73 N-terminal 111 residues form a globularly folded domain (hCDC73-NTD). We have determined a crystal structure of hCDC73-NTD at 1.02\u2009\u00c5 resolution, which reveals a novel protein fold. CDC73-NTD contains an extended hydrophobic groove on its surface that may be important for its function. Most pathogenic <jats:italic>CDC73<\/jats:italic> missense mutations associated with the HPT-JT syndrome are located in the region encoding CDC73-NTD. Our crystal and biochemical data indicate that most CDC73 missense mutations disrupt the folding of the hydrophobic core of hCDC73-NTD, while others such as the K34Q mutant reduce its thermostability. Overall, our results provide a solid structural basis for understanding the structure and function of CDC73 and its association with the HPT-JT syndrome and other diseases.<\/jats:p>","DOI":"10.1038\/s41598-017-15715-9","type":"journal-article","created":{"date-parts":[[2017,11,9]],"date-time":"2017-11-09T12:26:01Z","timestamp":1510230361000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Crystal structure of the N-terminal domain of human CDC73 and its implications for the hyperparathyroidism-jaw tumor (HPT-JT) syndrome"],"prefix":"10.1038","volume":"7","author":[{"given":"Wei","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Lin","family":"Kuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Ping","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Fei","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Xue","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqing","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,11,15]]},"reference":[{"key":"15715_CR1","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/ng1048","volume":"32","author":"JD Carpten","year":"2002","unstructured":"Carpten, J. D. et al. HRPT2, encoding parafibromin, is mutated in hyperparathyroidism-jaw tumor syndrome. Nature genetics \n                           32, 676\u2013680, https:\/\/doi.org\/10.1038\/ng1048 (2002).","journal-title":"Nature genetics"},{"key":"15715_CR2","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1111\/j.1365-2796.2009.02107.x","volume":"266","author":"PJ Newey","year":"2009","unstructured":"Newey, P. J., Bowl, M. R. & Thakker, R. V. Parafibromin - functional insights. Journal of Internal Medicine \n                           266, 84\u201398, https:\/\/doi.org\/10.1111\/j.1365-2796.2009.02107.x (2009).","journal-title":"Journal of Internal Medicine"},{"key":"15715_CR3","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1002\/humu.21188","volume":"31","author":"PJ Newey","year":"2010","unstructured":"Newey, P. J., Bowl, M. R., Cranston, T. & Thakker, R. V. Cell division cycle protein 73 homolog (CDC73) mutations in the hyperparathyroidism-jaw tumor syndrome (HPT-JT) and parathyroid tumors. Human mutation \n                           31, 295\u2013307, https:\/\/doi.org\/10.1002\/humu.21188 (2010).","journal-title":"Human mutation"},{"key":"15715_CR4","doi-asserted-by":"publisher","DOI":"10.1186\/s13000-016-0532-0","volume":"11","author":"Y Chen","year":"2016","unstructured":"Chen, Y. et al. CDC73 gene mutations in sporadic ossifying fibroma of the jaws. Diagnostic pathology \n                           11, 91, https:\/\/doi.org\/10.1186\/s13000-016-0532-0 (2016).","journal-title":"Diagnostic pathology"},{"key":"15715_CR5","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1007\/s12022-013-9294-3","volume":"25","author":"AJ Gill","year":"2014","unstructured":"Gill, A. J. Understanding the genetic basis of parathyroid carcinoma. Endocrine pathology \n                           25, 30\u201334, https:\/\/doi.org\/10.1007\/s12022-013-9294-3 (2014).","journal-title":"Endocrine pathology"},{"key":"15715_CR6","doi-asserted-by":"publisher","first-page":"3440","DOI":"10.1038\/sj.onc.1210131","volume":"26","author":"J Zhao","year":"2007","unstructured":"Zhao, J. et al. Sporadic human renal tumors display frequent allelic imbalances and novel mutations of the HRPT2 gene. Oncogene \n                           26, 3440\u20133449, https:\/\/doi.org\/10.1038\/sj.onc.1210131 (2007).","journal-title":"Oncogene"},{"key":"15715_CR7","doi-asserted-by":"publisher","first-page":"4204","DOI":"10.1210\/jcem.81.12.8954016","volume":"81","author":"BT Teh","year":"1996","unstructured":"Teh, B. T. et al. Autosomal dominant primary hyperparathyroidism and jaw tumor syndrome associated with renal hamartomas and cystic kidney disease: linkage to 1q21\u2013q32 and loss of the wild type allele in renal hamartomas. The Journal of clinical endocrinology and metabolism \n                           81, 4204\u20134211, https:\/\/doi.org\/10.1210\/jcem.81.12.8954016 (1996).","journal-title":"The Journal of clinical endocrinology and metabolism"},{"key":"15715_CR8","first-page":"944","volume":"56","author":"J Szabo","year":"1995","unstructured":"Szabo, J. et al. Hereditary hyperparathyroidism-jaw tumor syndrome: the endocrine tumor gene HRPT2 maps to chromosome 1q21-q31. American journal of human genetics \n                           56, 944\u2013950 (1995).","journal-title":"American journal of human genetics"},{"key":"15715_CR9","doi-asserted-by":"publisher","first-page":"E403","DOI":"10.1210\/jc.2012-2789","volume":"98","author":"L Bricaire","year":"2013","unstructured":"Bricaire, L. et al. Frequent large germline HRPT2 deletions in a French National cohort of patients with primary hyperparathyroidism. The Journal of clinical endocrinology and metabolism \n                           98, E403\u2013408, https:\/\/doi.org\/10.1210\/jc.2012-2789 (2013).","journal-title":"The Journal of clinical endocrinology and metabolism"},{"key":"15715_CR10","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1530\/EC-13-0046","volume":"2","author":"F Cetani","year":"2013","unstructured":"Cetani, F. et al. CDC73 mutational status and loss of parafibromin in the outcome of parathyroid cancer. Endocrine connections \n                           2, 186\u2013195, https:\/\/doi.org\/10.1530\/ec-13-0046 (2013).","journal-title":"Endocrine connections"},{"key":"15715_CR11","doi-asserted-by":"publisher","first-page":"2930","DOI":"10.1128\/MCB.00654-07","volume":"28","author":"PF Wang","year":"2008","unstructured":"Wang, P. F. et al. Parafibromin, a component of the human PAF complex, regulates growth factors and is required for embryonic development and survival in adult mice. Molecular and Cellular Biology \n                           28, 2930\u20132940, https:\/\/doi.org\/10.1128\/mcb.00654-07 (2008).","journal-title":"Molecular and Cellular Biology"},{"key":"15715_CR12","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15741","volume":"8","author":"Y Xu","year":"2017","unstructured":"Xu, Y. et al. Architecture of the RNA polymerase II-Paf1C-TFIIS transcription elongation complex. Nature communications \n                           8, 15741, https:\/\/doi.org\/10.1038\/ncomms15741 (2017).","journal-title":"Nature communications"},{"key":"15715_CR13","doi-asserted-by":"publisher","first-page":"669","DOI":"10.1128\/MCB.16.2.669","volume":"16","author":"X Shi","year":"1996","unstructured":"Shi, X. et al. Paf1p, an RNA polymerase II-associated factor in Saccharomyces cerevisiae, may have both positive and negative roles in transcription. Mol Cell Biol \n                           16, 669\u2013676 (1996).","journal-title":"Mol Cell Biol"},{"key":"15715_CR14","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1016\/j.cell.2009.12.050","volume":"140","author":"J Kim","year":"2010","unstructured":"Kim, J., Guermah, M. & Roeder, R. G. The Human PAF1 Complex Acts in Chromatin Transcription Elongation Both Independently and Cooperatively with SII\/TFIIS. Cell \n                           140, 491\u2013503, https:\/\/doi.org\/10.1016\/j.cell.2009.12.050 (2010).","journal-title":"Cell"},{"key":"15715_CR15","doi-asserted-by":"publisher","first-page":"10619","DOI":"10.1093\/nar\/gkt819","volume":"41","author":"X Chu","year":"2013","unstructured":"Chu, X. et al. Structural insights into Paf1 complex assembly and histone binding. Nucleic Acids Res \n                           41, 10619\u201310629, https:\/\/doi.org\/10.1093\/nar\/gkt819 (2013).","journal-title":"Nucleic Acids Res"},{"key":"15715_CR16","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/j.bbagrm.2012.08.011","volume":"1829","author":"BN Tomson","year":"2013","unstructured":"Tomson, B. N. & Arndt, K. M. The many roles of the conserved eukaryotic Paf1 complex in regulating transcription, histone modifications, and disease states. Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms \n                           1829, 116\u2013126, https:\/\/doi.org\/10.1016\/j.bbagrm.2012.08.011 (2013).","journal-title":"Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms"},{"key":"15715_CR17","doi-asserted-by":"publisher","first-page":"1272","DOI":"10.1038\/sj.onc.1208274","volume":"24","author":"GE Woodard","year":"2005","unstructured":"Woodard, G. E. et al. Parafibromin, product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2, regulates cyclin D1\/PRAD1 expression. Oncogene \n                           24, 1272\u20131276, https:\/\/doi.org\/10.1038\/sj.onc.1208274 (2005).","journal-title":"Oncogene"},{"key":"15715_CR18","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1093\/nar\/gkp991","volume":"38","author":"Y-J Yang","year":"2010","unstructured":"Yang, Y.-J., Han, J.-W., Youn, H.-D. & Cho, E.-J. The tumor suppressor, parafibromin, mediates histone H3 K9 methylation for cyclin D1 repression. Nucleic Acids Research \n                           38, 382\u2013390, https:\/\/doi.org\/10.1093\/nar\/gkp991 (2010).","journal-title":"Nucleic Acids Research"},{"key":"15715_CR19","doi-asserted-by":"publisher","first-page":"17420","DOI":"10.1073\/pnas.0710725105","volume":"105","author":"L Lin","year":"2008","unstructured":"Lin, L., Zhang, J. H., Panicker, L. M. & Simonds, W. F. The parafibromin tumor suppressor protein inhibits cell proliferation by repression of the c-myc proto-oncogene. Proceedings of the National Academy of Sciences of the United States of America \n                           105, 17420\u201317425, https:\/\/doi.org\/10.1073\/pnas.0710725105 (2008).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"15715_CR20","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1016\/j.cell.2006.01.053","volume":"125","author":"C Mosimann","year":"2006","unstructured":"Mosimann, C., Hausmann, G. & Basler, K. Parafibromin\/Hyrax activates Wnt\/Wg target gene transcription by direct association with beta-catenin\/Armadillo. Cell \n                           125, 327\u2013341, https:\/\/doi.org\/10.1016\/j.cell.2006.01.053 (2006).","journal-title":"Cell"},{"key":"15715_CR21","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/j.molcel.2011.05.014","volume":"43","author":"A Takahashi","year":"2011","unstructured":"Takahashi, A. et al. SHP2 Tyrosine Phosphatase Converts Parafibromin\/Cdc73 from a Tumor Suppressor to an Oncogenic Driver. Molecular Cell \n                           43, 45\u201356, https:\/\/doi.org\/10.1016\/j.molcel.2011.05.014 (2011).","journal-title":"Molecular Cell"},{"key":"15715_CR22","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12887","volume":"7","author":"I Kikuchi","year":"2016","unstructured":"Kikuchi, I. et al. Dephosphorylated parafibromin is a transcriptional coactivator of the Wnt\/Hedgehog\/Notch pathways. Nature communications \n                           7, 12887, https:\/\/doi.org\/10.1038\/ncomms12887 (2016).","journal-title":"Nature communications"},{"key":"15715_CR23","doi-asserted-by":"publisher","first-page":"394","DOI":"10.1016\/j.mod.2009.02.002","volume":"126","author":"C Mosimann","year":"2009","unstructured":"Mosimann, C., Hausmann, G. & Basler, K. The role of Parafibromin\/Hyrax as a nuclear Gli\/Ci-interacting protein in Hedgehog target gene control. Mechanisms of development \n                           126, 394\u2013405, https:\/\/doi.org\/10.1016\/j.mod.2009.02.002 (2009).","journal-title":"Mechanisms of development"},{"key":"15715_CR24","doi-asserted-by":"publisher","first-page":"10863","DOI":"10.1074\/jbc.M111.325647","volume":"287","author":"CG Amrich","year":"2012","unstructured":"Amrich, C. G. et al. Cdc73 subunit of Paf1 complex contains C-terminal Ras-like domain that promotes association of Paf1 complex with chromatin. The Journal of biological chemistry \n                           287, 10863\u201310875, https:\/\/doi.org\/10.1074\/jbc.M111.325647 (2012).","journal-title":"The Journal of biological chemistry"},{"key":"15715_CR25","doi-asserted-by":"publisher","first-page":"953","DOI":"10.1107\/S0907444912017325","volume":"68","author":"H Chen","year":"2012","unstructured":"Chen, H. et al. Crystallographic analysis of the conserved C-terminal domain of transcription factor Cdc73 from Saccharomyces cerevisiae reveals a GTPase-like fold. Acta crystallographica. Section D, Biological crystallography \n                           68, 953\u2013959, https:\/\/doi.org\/10.1107\/s0907444912017325 (2012).","journal-title":"Acta crystallographica. Section D, Biological crystallography"},{"key":"15715_CR26","doi-asserted-by":"publisher","first-page":"6241","DOI":"10.1038\/sj.onc.1208778","volume":"24","author":"MA Hahn","year":"2005","unstructured":"Hahn, M. A. & Marsh, D. J. Identification of a functional bipartite nuclear localization signal in the tumor suppressor parafibromin. Oncogene \n                           24, 6241\u20136248, https:\/\/doi.org\/10.1038\/sj.onc.1208778 (2005).","journal-title":"Oncogene"},{"key":"15715_CR27","doi-asserted-by":"publisher","first-page":"5070","DOI":"10.1016\/j.febslet.2007.09.050","volume":"581","author":"MA Hahn","year":"2007","unstructured":"Hahn, M. A. & Marsh, D. J. Nucleolar localization of parafibromin is mediated by three nucleolar localization signals. FEBS letters \n                           581, 5070\u20135074, https:\/\/doi.org\/10.1016\/j.febslet.2007.09.050 (2007).","journal-title":"FEBS letters"},{"key":"15715_CR28","doi-asserted-by":"publisher","unstructured":"Pazienza, V. et al. Identification and Functional Characterization of Three NoLS (Nucleolar Localisation Signals) Mutations of the CDC73 Gene. PloS one \n                           8, doi:https:\/\/doi.org\/10.1371\/journal.pone.0082292 (2013).","DOI":"10.1371\/journal.pone.0082292"},{"key":"15715_CR29","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1677\/ERC-06-0092","volume":"14","author":"F Cetani","year":"2007","unstructured":"Cetani, F. et al. Different somatic alterations of the HRPT2 gene in a patient with recurrent sporadic primary hyperparathyroidism carrying an HRPT2 germline mutation. Endocr Relat Cancer \n                           14, 493\u2013499, https:\/\/doi.org\/10.1677\/erc-06-0092 (2007).","journal-title":"Endocr Relat Cancer"},{"key":"15715_CR30","doi-asserted-by":"publisher","first-page":"e97994","DOI":"10.1371\/journal.pone.0097994","volume":"9","author":"G Masi","year":"2014","unstructured":"Masi, G. et al. Characterization of a new CDC73 missense mutation that impairs Parafibromin expression and nucleolar localization. PloS one \n                           9, e97994, https:\/\/doi.org\/10.1371\/journal.pone.0097994 (2014).","journal-title":"PloS one"},{"key":"15715_CR31","doi-asserted-by":"publisher","first-page":"612","DOI":"10.1128\/MCB.25.2.612-620.2005","volume":"25","author":"O Rozenblatt-Rosen","year":"2005","unstructured":"Rozenblatt-Rosen, O. et al. The parafibromin tumor suppressor protein is part of a human Paf1 complex. Molecular and Cellular Biology \n                           25, 612\u2013620, https:\/\/doi.org\/10.1128\/mcb.25.2.612-620.2005 (2005).","journal-title":"Molecular and Cellular Biology"},{"key":"15715_CR32","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1038\/embor.2009.14","volume":"10","author":"E Emmott","year":"2009","unstructured":"Emmott, E. & Hiscox, J. A. Nucleolar targeting: the hub of the matter. Embo Reports \n                           10, 231\u2013238, https:\/\/doi.org\/10.1038\/embor.2009.14 (2009).","journal-title":"Embo Reports"},{"key":"15715_CR33","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1083\/jcb.200812014","volume":"184","author":"T Pederson","year":"2009","unstructured":"Pederson, T. & Tsai, R. Y. L. In search of nonribosomal nucleolar protein function and regulation. Journal of Cell Biology \n                           184, 771\u2013776 (2009).","journal-title":"Journal of Cell Biology"},{"key":"15715_CR34","doi-asserted-by":"publisher","unstructured":"Walls, G. V. et al. Mice deleted for cell division cycle 73 gene develop parathyroid and uterine tumours: model for the hyperparathyroidism-jaw tumour syndrome. Oncogene, doi:https:\/\/doi.org\/10.1038\/onc.2017.43 (2017).","DOI":"10.1038\/onc.2017.43"},{"key":"15715_CR35","doi-asserted-by":"publisher","first-page":"6176","DOI":"10.1038\/sj.onc.1210445","volume":"26","author":"T Iwata","year":"2007","unstructured":"Iwata, T., Mizusawa, N., Taketani, Y., Itakura, M. & Yoshimoto, K. Parafibromin tumor suppressor enhances cell growth in the cells expressing SV40 large T antigen. Oncogene \n                           26, 6176\u20136183, https:\/\/doi.org\/10.1038\/sj.onc.1210445 (2007).","journal-title":"Oncogene"},{"key":"15715_CR36","doi-asserted-by":"publisher","first-page":"20582","DOI":"10.1074\/jbc.M109.017442","volume":"284","author":"J Kim","year":"2009","unstructured":"Kim, J. & Roeder, R. G. Direct Bre1-Paf1 complex interactions and RING finger-independent Bre1-Rad6 interactions mediate histone H2B ubiquitylation in yeast. The Journal of biological chemistry \n                           284, 20582\u201320592, https:\/\/doi.org\/10.1074\/jbc.M109.017442 (2009).","journal-title":"The Journal of biological chemistry"},{"key":"15715_CR37","doi-asserted-by":"publisher","first-page":"34739","DOI":"10.1074\/jbc.C300269200","volume":"278","author":"A Wood","year":"2003","unstructured":"Wood, A., Schneider, J., Dover, J., Johnston, M. & Shilatifard, A. The Paf1 complex is essential for histone monoubiquitination by the Rad6-Bre1 complex, which signals for histone methylation by COMPASS and Dot1p. The Journal of biological chemistry \n                           278, 34739\u201334742, https:\/\/doi.org\/10.1074\/jbc.C300269200 (2003).","journal-title":"The Journal of biological chemistry"},{"key":"15715_CR38","doi-asserted-by":"publisher","first-page":"1213","DOI":"10.1038\/sj.onc.1209893","volume":"26","author":"KJ Bradley","year":"2007","unstructured":"Bradley, K. J. et al. Parafibromin is a nuclear protein with a functional monopartite nuclear localization signal. Oncogene \n                           26, 1213\u20131221, https:\/\/doi.org\/10.1038\/sj.onc.1209893 (2007).","journal-title":"Oncogene"},{"key":"15715_CR39","doi-asserted-by":"publisher","first-page":"859","DOI":"10.1107\/S0907444906019949","volume":"62","author":"W Minor","year":"2006","unstructured":"Minor, W., Cymborowski, M., Otwinowski, Z. & Chruszcz, M. HKL-3000: the integration of data reduction and structure solution - from diffraction images to an initial model in minutes. Acta Crystallographica Section D-Biological Crystallography \n                           62, 859\u2013866, https:\/\/doi.org\/10.1107\/s0907444906019949 (2006).","journal-title":"Acta Crystallographica Section D-Biological Crystallography"},{"key":"15715_CR40","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"PD Adams","year":"2010","unstructured":"Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta crystallographica. Section D, Biological crystallography \n                           66, 213\u2013221, https:\/\/doi.org\/10.1107\/s0907444909052925 (2010).","journal-title":"Acta crystallographica. Section D, Biological crystallography"},{"key":"15715_CR41","doi-asserted-by":"publisher","unstructured":"The CCP4 suite: programs for protein crystallography. Acta crystallographica. Section D, Biological crystallography \n                           50, 760-763, doi:https:\/\/doi.org\/10.1107\/s0907444994003112 (1994).","DOI":"10.1107\/s0907444994003112"},{"key":"15715_CR42","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1107\/S0907444910007493","volume":"66","author":"P Emsley","year":"2010","unstructured":"Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta crystallographica. Section D, Biological crystallography \n                           66, 486\u2013501, https:\/\/doi.org\/10.1107\/s0907444910007493 (2010).","journal-title":"Acta crystallographica. Section D, Biological crystallography"},{"key":"15715_CR43","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1002\/prot.340200202","volume":"20","author":"WL DeLano","year":"1994","unstructured":"DeLano, W. L. & Brunger, A. T. Helix packing in proteins: prediction and energetic analysis of dimeric, trimeric, and tetrameric GCN4 coiled coil structures. Proteins \n                           20, 105\u2013123, https:\/\/doi.org\/10.1002\/prot.340200202 (1994).","journal-title":"Proteins"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-15715-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-15715-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-15715-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T13:47:05Z","timestamp":1671803225000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-15715-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,15]]},"references-count":43,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["15715"],"URL":"https:\/\/doi.org\/10.1038\/s41598-017-15715-9","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,15]]},"assertion":[{"value":"12 September 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 October 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 November 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"15638"}}