{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:08:55Z","timestamp":1790626135704,"version":"4.1.0"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,11,21]],"date-time":"2017-11-21T00:00:00Z","timestamp":1511222400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,11,21]],"date-time":"2017-11-21T00:00:00Z","timestamp":1511222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The process, how lipids are removed from the circulation and transferred from high density lipoprotein (HDL) \u2013 a main carrier of cholesterol in the blood stream \u2013 to cells, is highly complex. HDL particles are captured from the blood stream by the scavenger receptor, class B, type I (SR-BI), the so-called HDL receptor. The details in subsequent lipid-transfer process, however, have not yet been completely understood. The transfer has been proposed to occur directly at the cell surface across an unstirred water layer, via a hydrophobic channel in the receptor, or after HDL endocytosis. The role of the target lipid membrane for the transfer process, however, has largely been overlooked. Here, we studied at the single molecule level how HDL particles interact with synthetic lipid membranes. Using (high-speed)\u00a0atomic force microscopy and fluorescence correlation spectroscopy (FCS) we found out that, upon contact with the membrane, HDL becomes integrated into the lipid bilayer. Combined force and single molecule fluorescence microscopy allowed us to directly monitor the transfer process of fluorescently labelled amphiphilic lipid probe from HDL particles to the lipid bilayer upon contact.<\/jats:p>","DOI":"10.1038\/s41598-017-15949-7","type":"journal-article","created":{"date-parts":[[2017,11,15]],"date-time":"2017-11-15T04:45:28Z","timestamp":1510721128000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["HDL particles incorporate into lipid bilayers \u2013 a combined AFM and single molecule fluorescence microscopy study"],"prefix":"10.1038","volume":"7","author":[{"given":"Birgit","family":"Plochberger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1192-0748","authenticated-orcid":false,"given":"Clemens","family":"R\u00f6hrl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Preiner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Rankl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8672-2255","authenticated-orcid":false,"given":"Mario","family":"Brameshuber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3637-2546","authenticated-orcid":false,"given":"Josef","family":"Madl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Bittman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Ros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4915-388X","authenticated-orcid":false,"given":"Erdinc","family":"Sezgin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Eggeling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Hinterdorfer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7288-7320","authenticated-orcid":false,"given":"Herbert","family":"Stangl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1542-1089","authenticated-orcid":false,"given":"Gerhard J.","family":"Sch\u00fctz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,11,21]]},"reference":[{"key":"15949_CR1","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1038\/nrm2336","volume":"9","author":"E Ikonen","year":"2008","unstructured":"Ikonen, E. Cellular cholesterol trafficking and compartmentalization. Nat Rev Mol Cell Biol \n                           9, 125\u2013138, https:\/\/doi.org\/10.1038\/nrm2336 (2008).","journal-title":"Nat Rev Mol Cell Biol"},{"key":"15949_CR2","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1146\/annurev.biophys.093008.131357","volume":"39","author":"JPR Lippincott-Schwartz","year":"2010","unstructured":"Lippincott-Schwartz, J. P. R. Lipids and cholesterol as regulators of traffic in the endomembrane system. Annu. Rev. Biophys. \n                           39, 559\u2013578 (2010).","journal-title":"Annu. Rev. Biophys."},{"key":"15949_CR3","doi-asserted-by":"publisher","first-page":"1905","DOI":"10.1161\/CIRCULATIONAHA.111.066589","volume":"125","author":"RS Rosenson","year":"2012","unstructured":"Rosenson, R. S. et al. Cholesterol efflux and atheroprotection: advancing the concept of reverse cholesterol transport. Circulation \n                           125, 1905\u20131919, https:\/\/doi.org\/10.1161\/CIRCULATIONAHA.111.066589 (2012).","journal-title":"Circulation"},{"key":"15949_CR4","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1038\/nrendo.2011.235","volume":"8","author":"BG Drew","year":"2012","unstructured":"Drew, B. G., Rye, K. A., Duffy, S. J., Barter, P. & Kingwell, B. A. The emerging role of HDL in glucose metabolism. Nature reviews. Endocrinology \n                           8, 237\u2013245, https:\/\/doi.org\/10.1038\/nrendo.2011.235 (2012).","journal-title":"Nature reviews. Endocrinology"},{"key":"15949_CR5","doi-asserted-by":"publisher","first-page":"119","DOI":"10.3389\/fphar.2013.00119","volume":"4","author":"PM Cruz","year":"2013","unstructured":"Cruz, P. M., Mo, H., McConathy, W. J., Sabnis, N. & Lacko, A. G. The role of cholesterol metabolism and cholesterol transport in carcinogenesis: a review of scientific findings, relevant to future cancer therapeutics. 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Combined light and electron microscopy using diaminobenzidine photooxidation to monitor trafficking of lipids derived from lipoprotein particles. Curr Pharm Biotechnol \n                           13, 331\u2013340, doi:BSP\/CPB\/E-Pub\/000195-12-12 [pii] (2012).","DOI":"10.2174\/138920112799095338"},{"key":"15949_CR25","doi-asserted-by":"publisher","first-page":"e12811","DOI":"10.1371\/journal.pone.0012811","volume":"5","author":"H Khandelia","year":"2010","unstructured":"Khandelia, H., Duelund, L., Pakkanen, K. I. & Ipsen, J. H. Triglyceride blisters in lipid bilayers: implications for lipid droplet biogenesis and the mobile lipid signal in cancer cell membranes. PLoS ONE \n                           5, e12811, https:\/\/doi.org\/10.1371\/journal.pone.0012811 (2010).","journal-title":"PLoS ONE"},{"key":"15949_CR26","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1083\/jcb.201305142","volume":"203","author":"A Kassan","year":"2013","unstructured":"Kassan, A. et al. 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Methods \n                           46, 131\u2013140 (2008).","journal-title":"Methods"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-15949-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-15949-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-15949-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T08:53:05Z","timestamp":1671785585000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-15949-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,21]]},"references-count":40,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["15949"],"URL":"https:\/\/doi.org\/10.1038\/s41598-017-15949-7","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,21]]},"assertion":[{"value":"21 July 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 November 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 November 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"15886"}}