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Despite its impact on human health, <jats:italic>Cryptosporidium<\/jats:italic> pathogenesis remains poorly known, mainly due to the lack of a long-term culture method for this parasite. Thus, the aim of the present study was to develop a three-dimensional (3D) culture model from adult murine colon allowing biological investigations of the host-parasite interactions in an <jats:italic>in vivo-<\/jats:italic>like environment and, in particular, the development of parasite-induced neoplasia. Colonic explants were cultured and preserved <jats:italic>ex vivo<\/jats:italic> for 35 days and co-culturing was performed with <jats:italic>C<\/jats:italic>. <jats:italic>parvum<\/jats:italic>. Strikingly, the resulting system allowed the reproduction of neoplastic lesions <jats:italic>in vitro<\/jats:italic> at 27 days post-infection (PI), providing new evidence of the role of the parasite in the induction of carcinogenesis. This promising model could facilitate the study of host-pathogen interactions and the investigation of the process involved in <jats:italic>Cryptosporidium<\/jats:italic>-induced cell transformation.<\/jats:p>","DOI":"10.1038\/s41598-017-17304-2","type":"journal-article","created":{"date-parts":[[2017,12,5]],"date-time":"2017-12-05T09:47:31Z","timestamp":1512467251000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Three-dimensional (3D) culture of adult murine colon as an in vitro model of cryptosporidiosis: Proof of concept"],"prefix":"10.1038","volume":"7","author":[{"given":"Martha","family":"Baydoun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadia Benamrouz","family":"Vanneste","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colette","family":"Creusy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Guyot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nausicaa","family":"Gantois","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6732-6772","authenticated-orcid":false,"given":"Magali","family":"Chabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baptiste","family":"Delaire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Mouray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atallah","family":"Baydoun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"Forzy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Chieux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Gosset","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Senez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Viscogliosi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Follet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Certad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,12,11]]},"reference":[{"key":"17304_CR1","doi-asserted-by":"publisher","first-page":"1667","DOI":"10.1017\/S0031182014001085","volume":"141","author":"U Ryan","year":"2014","unstructured":"Ryan, U., Fayer, R. & Xiao, L. 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