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The members of the second clade, \u201c<jats:italic>Listeria sensu lato<\/jats:italic>\u201d, are believed to be solely environmental bacteria without the ability to colonize mammalian hosts. To identify novel determinants that contribute to infection by<jats:italic>L. monocytogenes<\/jats:italic>, the causative agent of the foodborne disease listeriosis, we performed a genome comparison of the two clades and found 151 candidate genes that are conserved in the<jats:italic>Listeria sensu stricto<\/jats:italic>species. Two factors were investigated further<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>. A mutant lacking an ATP-binding cassette transporter exhibited defective adhesion and invasion of human Caco-2 cells. Using a mouse model of foodborne<jats:italic>L. monocytogenes<\/jats:italic>infection, a reduced number of the mutant strain compared to the parental strain was observed in the small intestine and the liver. Another mutant with a defective 1,2-propanediol degradation pathway showed reduced persistence in the stool of infected mice, suggesting a role of 1,2-propanediol as a carbon and energy source of listeriae during infection. These findings reveal the relevance of novel factors for the colonization process of<jats:italic>L. monocytogenes<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/s41598-017-17570-0","type":"journal-article","created":{"date-parts":[[2017,12,13]],"date-time":"2017-12-13T12:29:57Z","timestamp":1513168197000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Comparison between Listeria sensu stricto and Listeria sensu lato strains identifies novel determinants involved in infection"],"prefix":"10.1038","volume":"7","author":[{"given":"Jakob","family":"Schardt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grant","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie","family":"M\u00fcller-Herbst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1453-9002","authenticated-orcid":false,"given":"Kristina","family":"Schauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah E. 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