{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:47:57Z","timestamp":1790711277140,"version":"4.1.0"},"reference-count":54,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,12,15]],"date-time":"2017-12-15T00:00:00Z","timestamp":1513296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,12,15]],"date-time":"2017-12-15T00:00:00Z","timestamp":1513296000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Tuberculosis (TB) is currently the number one killer among infectious diseases worldwide. Lipids are abundant molecules during the infectious cycle of\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    (\n                    <jats:italic>Mtb<\/jats:italic>\n                    ) and studies better mimicking its actual metabolic state during pathogenesis are needed. Though most studies have focused on the mycobacterial lipid metabolism under standard culture conditions, little is known about the transcriptome of\n                    <jats:italic>Mtb<\/jats:italic>\n                    in a lipid environment. Here we determined the transcriptome of\n                    <jats:italic>Mtb<\/jats:italic>\n                    H37Rv in a lipid-rich environment (cholesterol and fatty acid) under aerobic and hypoxic conditions, using RNAseq. Lipids significantly induced the expression of 368 genes. A main core lipid response was observed involving efflux systems, iron caption and sulfur reduction. In co-expression with ncRNAs and other genes discussed below, may act coordinately to prepare the machinery conferring drug tolerance and increasing a persistent population. Our findings could be useful to tag relevant pathways for the development of new drugs, vaccines and new strategies to control TB.\n                  <\/jats:p>","DOI":"10.1038\/s41598-017-17751-x","type":"journal-article","created":{"date-parts":[[2017,12,11]],"date-time":"2017-12-11T10:23:11Z","timestamp":1512987791000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["The transcriptome of Mycobacterium tuberculosis in a lipid-rich dormancy model through RNAseq analysis"],"prefix":"10.1038","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5280-4260","authenticated-orcid":false,"given":"Diana A.","family":"Aguilar-Ayala","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7584-1179","authenticated-orcid":false,"given":"Laurentijn","family":"Tilleman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8815-5485","authenticated-orcid":false,"given":"Filip","family":"Van Nieuwerburgh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0635-661X","authenticated-orcid":false,"given":"Dieter","family":"Deforce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"Palomino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Vandamme","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6461-3135","authenticated-orcid":false,"given":"Jorge A.","family":"Gonzalez-Y-Merchand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anandi","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,12,15]]},"reference":[{"key":"17751_CR1","unstructured":"WHO. 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Tuberculosis \n                           103, 1\u20139 (2017).","journal-title":"Tuberculosis"},{"key":"17751_CR4","doi-asserted-by":"crossref","unstructured":"Shleeva, M. et al. Cyclic AMP-dependent resuscitation of dormant mycobacteria by exogenous free fatty acids. PLoS One \n                           8, (2013).","DOI":"10.1371\/journal.pone.0082914"},{"key":"17751_CR5","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.mib.2015.10.002","volume":"29","author":"RR Lovewell","year":"2016","unstructured":"Lovewell, R. R., Sassetti, C. M. & VanderVen, B. C. Chewing the fat: lipid metabolism and homeostasis during M. tuberculosis infection. Curr. Opin. Microbiol. \n                           29, 30\u201336 (2016).","journal-title":"Curr. Opin. Microbiol."},{"key":"17751_CR6","doi-asserted-by":"publisher","first-page":"392","DOI":"10.1016\/j.meegid.2016.10.015","volume":"55","author":"DA Aguilar-Ayala","year":"2017","unstructured":"Aguilar-Ayala, D. A., Palomino, J. C., Vandamme, P., Martin, A. & Gonzalez-y-Merchand, J. A. \u2018Genetic regulation of Mycobacterium tuberculosis in a lipid-rich environment\u2019. Infect. Genet. Evol. \n                           55, 392\u2013402 (2017).","journal-title":"Infect. Genet. Evol."},{"key":"17751_CR7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.chom.2013.12.005","volume":"15","author":"MS Siegrist","year":"2014","unstructured":"Siegrist, M. S. & Bertozzi, C. R. Mycobacterial lipid logic. Cell Host Microbe \n                           15, 1\u20132 (2014).","journal-title":"Cell Host Microbe"},{"key":"17751_CR8","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1111\/j.1365-3083.2009.02295.x","volume":"70","author":"S Mu\u00f1oz","year":"2009","unstructured":"Mu\u00f1oz, S., Rivas-Santiago, B. & Enciso, J. A. Mycobacterium tuberculosis entry into mast cells through cholesterol-rich membrane microdomains. Scand. J. 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