{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T02:09:32Z","timestamp":1767924572581,"version":"3.49.0"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,12,14]],"date-time":"2017-12-14T00:00:00Z","timestamp":1513209600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,12,14]],"date-time":"2017-12-14T00:00:00Z","timestamp":1513209600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Kashin-Beck disease (KBD) is a deformative, endemic osteochondropathy involving degeneration and necrosis of growth plates and articular cartilage. The pathogenesis of KBD is related to gene expression and regulation mechanisms, but long noncoding RNAs (lncRNAs) in KBD have not been investigated. In this study, we identified 316 up-regulated and 631 down-regulated lncRNAs (\u2265\u00a02-fold change) in KBD chondrocytes using microarray analysis, of which more than three-quarters were intergenic lncRNAs and antisense lncRNAs. We also identified 232 up-regulated and 427 down-regulated mRNAs (\u2265\u00a02-fold change). A lncRNA-mRNA correlation analysis combined 343 lncRNAs and 292 mRNAs to form 509 coding-noncoding gene co-expression networks (CNC networks). Eleven lncRNAs were predicted to have cis-regulated target genes, including NAV2 (neuron navigator 2), TOX (thymocyte selection-associated high mobility group box), LAMA4 (laminin, alpha 4), and DEPTOR (DEP domain containing mTOR-interacting protein). The differentially expressed mRNAs in KBD significantly contribute to biological events associated with the extracellular matrix. Meanwhile, 34 mRNAs and 55 co-expressed lncRNAs constituted a network that influences the extracellular matrix. In the network, FBLN1 and LAMA 4 were the core genes with the highest significance. These novel findings indicate that lncRNAs may play a role in extracellular matrix destruction in KBD.<\/jats:p>","DOI":"10.1038\/s41598-017-17875-0","type":"journal-article","created":{"date-parts":[[2017,12,8]],"date-time":"2017-12-08T09:42:56Z","timestamp":1512726176000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Long noncoding RNA expression profile reveals lncRNAs signature associated with extracellular matrix degradation in kashin-beck disease"],"prefix":"10.1038","volume":"7","author":[{"given":"Cuiyan","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng\u2019e","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanzhen","family":"Shao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yujie","family":"Ning","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guanghui","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Byeong Jae","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6181-9904","authenticated-orcid":false,"given":"Mikko","family":"Lammi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiong","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,12,14]]},"reference":[{"key":"17875_CR1","doi-asserted-by":"publisher","first-page":"1774","DOI":"10.1016\/j.joca.2014.07.023","volume":"22","author":"X Guo","year":"2014","unstructured":"Guo, X. et al. Recent advances in the research of an endemic osteochondropathy in China: Kashin-Beck disease. Osteoarthritis and Cartilage \n                           22, 1774\u20131783, https:\/\/doi.org\/10.1016\/j.joca.2014.07.023 (2014).","journal-title":"Osteoarthritis and Cartilage"},{"key":"17875_CR2","doi-asserted-by":"publisher","first-page":"868","DOI":"10.1016\/j.joca.2015.01.009","volume":"23","author":"Q Fu","year":"2015","unstructured":"Fu, Q. et al. Radiographic features of hand osteoarthritis in adult Kashin-Beck Disease (KBD): the Yongshou KBD study. Osteoarthritis and Cartilage \n                           23, 868\u2013873, https:\/\/doi.org\/10.1016\/j.joca.2015.01.009 (2015).","journal-title":"Osteoarthritis and Cartilage"},{"key":"17875_CR3","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1007\/s002640000190","volume":"25","author":"JL Pasteels","year":"2001","unstructured":"Pasteels, J. L. et al. Histology of Kashin-Beck lesions. International Orthopaedics \n                           25, 151\u2013153 (2001).","journal-title":"International Orthopaedics"},{"key":"17875_CR4","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1039\/C5TX00377F","volume":"5","author":"D Li","year":"2016","unstructured":"Li, D. et al. The effects of T-2 toxin on the prevalence and development of Kashin-Beck disease in China: a meta-analysis and systematic review. Toxicology Research \n                           5, 731\u2013751, https:\/\/doi.org\/10.1039\/c5tx00377f (2016).","journal-title":"Toxicology Research"},{"key":"17875_CR5","doi-asserted-by":"publisher","first-page":"1171","DOI":"10.1016\/j.joca.2007.03.013","volume":"15","author":"FL Ren","year":"2007","unstructured":"Ren, F. L. et al. Effects of selenium and iodine deficiency on bone, cartilage growth plate and chondrocyte differentiation in two generations of rats. Osteoarthritis and Cartilage \n                           15, 1171\u20131177, https:\/\/doi.org\/10.1016\/j.joca.2007.03.013 (2007).","journal-title":"Osteoarthritis and Cartilage"},{"key":"17875_CR6","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1111\/j.1365-2443.2012.01620.x","volume":"17","author":"S Wang","year":"2012","unstructured":"Wang, S., Guo, X., Wang, W. & Wang, S. Genome-wide study identifies the regulatory gene networks and signaling pathways from chondrocyte and peripheral blood monocyte of Kashin-Beck disease. Genes to Cells \n                           17, 619\u2013632, https:\/\/doi.org\/10.1111\/j.1365-2443.2012.01620.x (2012).","journal-title":"Genes to Cells"},{"key":"17875_CR7","doi-asserted-by":"publisher","unstructured":"Yu, F. F. et al. Identified molecular mechanism of interaction between environmental risk factors and differential expression genes in cartilage of Kashin-Beck disease. Medicine \n                           95, https:\/\/doi.org\/10.1097\/md.0000000000005669 (2016).","DOI":"10.1097\/md.0000000000005669"},{"key":"17875_CR8","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1016\/j.joca.2010.02.004","volume":"18","author":"YM Xiong","year":"2010","unstructured":"Xiong, Y. M. et al. Association study between polymorphisms in selenoprotein genes and susceptibility to Kashin-Beck disease. Osteoarthritis and Cartilage \n                           18, 817\u2013824, https:\/\/doi.org\/10.1016\/j.joca.2010.02.004 (2010).","journal-title":"Osteoarthritis and Cartilage"},{"key":"17875_CR9","doi-asserted-by":"publisher","unstructured":"Wang, S., Guo, X., Wu, X.-m. & Lammi, M. J. Genome-Wide Gene Expression Analysis Suggests an Important Role of Suppressed Immunity in Pathogenesis of Kashin-Beck Disease. 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Rheumatology International \n                           32, 3503\u20133509, https:\/\/doi.org\/10.1007\/s00296-011-2172-8 (2012).","journal-title":"Rheumatology International"},{"key":"17875_CR34","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1159\/000335046","volume":"196","author":"M Lu","year":"2012","unstructured":"Lu, M. et al. The Effects of Mycotoxins and Selenium Deficiency on Tissue-Engineered Cartilage. Cells Tissues Organs \n                           196, 241\u2013250, https:\/\/doi.org\/10.1159\/000335046 (2012).","journal-title":"Cells Tissues Organs"},{"key":"17875_CR35","first-page":"381","volume":"26","author":"J-h Chen","year":"2006","unstructured":"Chen, J.-h et al. Protective effect of selenium against T-2 toxin-induced inhibition of chondrocyte aggrecan and collagen II synthesis. Journal of Southern Medical University \n                           26, 381\u2013385 (2006).","journal-title":"Journal of Southern Medical University"},{"issue":"272-274","key":"17875_CR36","first-page":"278","volume":"32","author":"Y Haojie","year":"2011","unstructured":"Haojie, Y. et al. Effects of T-2 toxin on the mRNA expression of apoptosis-related gene in articular chondrocytes of selenium-deficiency rats. Journal of Xi\u2019an Jiaotong University. Medical Sciences \n                           32(272\u2013274), 278 (2011).","journal-title":"Medical Sciences"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-17875-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-17875-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-17875-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T16:20:15Z","timestamp":1671812415000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-17875-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,14]]},"references-count":36,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["17875"],"URL":"https:\/\/doi.org\/10.1038\/s41598-017-17875-0","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,14]]},"assertion":[{"value":"31 July 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 December 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 December 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"17553"}}