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Various skin substitutes have been developed to resolve this issue with minimal success. In this study, to yield a novel construct for<jats:italic>in situ<\/jats:italic>regeneration of sweat glands, the collagen-chitosan porous scaffold was combined with Lipofectamine 2000\/pDNA-EGF complexes to obtain the gene-activated scaffold (GAS), which was then seeded with bone marrow-derived mesenchymal stem cells (BM-MSCs). The porous scaffold functionalized as a reservoir for the incorporated gene complexes which were released in a sustained manner. The seeded BM-MSCs were transfected<jats:italic>in situ<\/jats:italic>by the released complexes and specially differentiated into sweat gland cells<jats:italic>in vitro<\/jats:italic>under the induction of the expressed epidermal growth factor (EGF). Application<jats:italic>in vivo<\/jats:italic>of the GAS\/BM-MSCs constructs on the full-thickness skin defects of SD rats confirmed that GAS\/BM-MSCs could accelerate the wound healing process and induce the<jats:italic>in situ<\/jats:italic>regeneration of the full-thickness skin with sweat gland-like structures. Analyzed by immunohistochemical staining, RT-qPCR and Western-blotting, the levels of the major sweat gland markers such as carcino-embryonic antigen (CEA), cytokeratin 8 (CK8) and cytokeratin 14 (CK14) were all up-regulated, indicating that GAS\/BM-MSCs can facilitate the regeneration of sweat glands-like structure<jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/s41598-017-17967-x","type":"journal-article","created":{"date-parts":[[2017,12,11]],"date-time":"2017-12-11T10:44:33Z","timestamp":1512989073000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Gene-activated matrix\/bone marrow-derived mesenchymal stem cells constructs regenerate sweat glands-like structure in vivo"],"prefix":"10.1038","volume":"7","author":[{"given":"Pranish","family":"Kolakshyapati","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuyuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunye","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxia","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiqiang","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lie","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changyou","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,12,15]]},"reference":[{"key":"17967_CR1","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1586\/17434440.3.3.373","volume":"3","author":"C Hrabchak","year":"2006","unstructured":"Hrabchak, C., Flynn, L. & Woodhouse, K. 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