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CF is characterized by repeated lung infections leading to respiratory failure. Using a high-throughput method, we developed a T7 phage display cDNA library derived from mRNA isolated from bronchoalveolar lavage (BAL) cells and leukocytes of sarcoidosis patients. This library was biopanned to obtain 1070 potential antigens. A microarray platform was constructed and immunoscreened with sera from healthy (n\u2009=\u200949), lung cancer (LC) (n\u2009=\u200931) and CF (n\u2009=\u200931) subjects. We built 1,000 na\u00efve Bayes models on the training sets. We selected the top 20 frequently significant clones ranked with student <jats:italic>t<\/jats:italic>-test discriminating CF antigens from healthy controls and LC at a False Discovery Rate (FDR)\u2009&lt;\u20090.01. The performances of the models were validated on an independent validation set. The mean of the area under the receiver operating characteristic (ROC) curve for the classifiers was 0.973 with a sensitivity of 0.999 and specificity of 0.959. Finally, we identified CF specific clones that correlate highly with sweat chloride test, BMI, and FEV1% predicted values. For the first time, we show that CF specific serological biomarkers can be identified through immunocreenings of a T7 phage display library with high accuracy, which may have utility in development of molecular therapy.<\/jats:p>","DOI":"10.1038\/s41598-017-18041-2","type":"journal-article","created":{"date-parts":[[2017,12,12]],"date-time":"2017-12-12T19:26:45Z","timestamp":1513106805000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Detection of Cystic Fibrosis Serological Biomarkers Using a T7 Phage Display Library"],"prefix":"10.1038","volume":"7","author":[{"given":"Harvinder","family":"Talwar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samer Najeeb","family":"Hanoudi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreea","family":"Geamanu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dana","family":"Kissner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sorin","family":"Draghici","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3327-2585","authenticated-orcid":false,"given":"Lobelia","family":"Samavati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,12,18]]},"reference":[{"key":"18041_CR1","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1056\/NEJMra1300109","volume":"372","author":"DA Stoltz","year":"2015","unstructured":"Stoltz, D. 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