{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T03:50:53Z","timestamp":1793764253847,"version":"4.6.1"},"reference-count":56,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2018,1,11]],"date-time":"2018-01-11T00:00:00Z","timestamp":1515628800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2018,1,11]],"date-time":"2018-01-11T00:00:00Z","timestamp":1515628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hitherto, the known mechanisms underpinning cell-fate specification act on neural progenitors, affecting their commitment to generate neuron or glial cells. Here, we show that particular phospholipids supplemented in the culture media modify the commitment of post-mitotic neural cells <jats:italic>in vitro<\/jats:italic>. Phosphatidylcholine (PtdCho)-enriched media enhances neuronal differentiation at the expense of astroglial and unspecified cells. Conversely, phosphatidylethanolamine (PtdEtn) enhances astroglial differentiation and accelerates astrocyte maturation. The ability of phospholipids to modify the fate of post-mitotic cells depends on its presence during a narrow time-window during cell differentiation and it is mediated by the selective activation of particular signaling pathways. While PtdCho-mediated effect on neuronal differentiation depends on cAMP-dependent kinase (PKA)\/calcium responsive element binding protein (CREB), PtdEtn stimulates astrogliogenesis through the activation of the MEK\/ERK signaling pathway. Collectively, our results provide an additional degree of plasticity in neural cell specification and further support the notion that cell differentiation is a reversible phenomenon. They also contribute to our understanding of neuronal and glial lineage specification in the central nervous system, opening up new avenues to retrieve neurogenic capacity in the brain.<\/jats:p>","DOI":"10.1038\/s41598-017-18700-4","type":"journal-article","created":{"date-parts":[[2018,1,5]],"date-time":"2018-01-05T11:14:10Z","timestamp":1515150850000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells"],"prefix":"10.1038","volume":"8","author":[{"given":"Aneley","family":"Montaner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Themis Taynah","family":"da Silva Santana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timm","family":"Schroeder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo","family":"Einicker-Lamas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Girardini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4928-2163","authenticated-orcid":false,"given":"Marcos Romualdo","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Banchio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,1,11]]},"reference":[{"key":"18700_CR1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/dbio.1996.0090","volume":"175","author":"BA Reynolds","year":"1996","unstructured":"Reynolds, B. A. & Weiss, S. Clonal and population analyses demonstrate that an EGF-responsive mammalian embryonic CNS precursor is a stem cell. Dev Biol 175, 1\u201313 (1996).","journal-title":"Dev Biol"},{"key":"18700_CR2","doi-asserted-by":"crossref","first-page":"4565","DOI":"10.1523\/JNEUROSCI.12-11-04565.1992","volume":"12","author":"BA Reynolds","year":"1992","unstructured":"Reynolds, B. A., Tetzlaff, W. & Weiss, S. A multipotent EGF-responsive striatal embryonic progenitor cell produces neurons and astrocytes. J Neurosci 12, 4565\u20134574 (1992).","journal-title":"J Neurosci"},{"key":"18700_CR3","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1038\/nrm1739","volume":"6","author":"M Gotz","year":"2005","unstructured":"Gotz, M. & Huttner, W. B. The cell biology of neurogenesis. Nat Rev Mol Cell Biol 6, 777\u2013788 (2005).","journal-title":"Nat Rev Mol Cell Biol"},{"key":"18700_CR4","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.neuron.2007.04.019","volume":"54","author":"FD Miller","year":"2007","unstructured":"Miller, F. D. & Gauthier, A. S. Timing is everything: making neurons versus glia in the developing cortex. Neuron 54, 357\u2013369 (2007).","journal-title":"Neuron"},{"key":"18700_CR5","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.stemcr.2017.05.009","volume":"9","author":"M Chouchane","year":"2017","unstructured":"Chouchane, M. et al. Lineage Reprogramming of Astroglial Cells from Different Origins into Distinct Neuronal Subtypes. Stem Cell Reports 9, 162\u2013176 (2017).","journal-title":"Stem Cell Reports"},{"key":"18700_CR6","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/j.neuron.2015.01.009","volume":"85","author":"YC Yung","year":"2015","unstructured":"Yung, Y. C., Stoddard, N. C., Mirendil, H. & Chun, J. Lysophosphatidic Acid signaling in the nervous system. Neuron 85, 669\u2013682 (2015).","journal-title":"Neuron"},{"key":"18700_CR7","volume":"6","author":"YB Zhu","year":"2016","unstructured":"Zhu, Y. B. et al. Astrocyte-derived phosphatidic acid promotes dendritic branching. Sci Rep 6, 21096 (2016).","journal-title":"Sci Rep"},{"key":"18700_CR8","doi-asserted-by":"crossref","first-page":"6316","DOI":"10.1007\/s12035-015-9528-0","volume":"53","author":"L Paoletti","year":"2016","unstructured":"Paoletti, L. et al. Lysophosphatidylcholine Drives Neuroblast Cell Fate. Mol Neurobiol 53, 6316\u20136331 (2016).","journal-title":"Mol Neurobiol"},{"key":"18700_CR9","doi-asserted-by":"crossref","unstructured":"Wang, Q. et al. Effects of ganglioside GM1 and neural growth factor on neural stem cell proliferation and differentiation. Genet Mol Res 15 (2016).","DOI":"10.4238\/gmr.15038376"},{"key":"18700_CR10","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/S0306-4522(03)00198-2","volume":"119","author":"E Hogyes","year":"2003","unstructured":"Hogyes, E. et al. Neuroprotective effect of developmental docosahexaenoic acid supplement against excitotoxic brain damage in infant rats. Neuroscience 119, 999\u20131012 (2003).","journal-title":"Neuroscience"},{"key":"18700_CR11","doi-asserted-by":"crossref","unstructured":"Marcucci, H., Paoletti, L., Jackowski, S. & Banchio, C. Phosphatidylcholine biosynthesis during neuronal differentiation and its role in cell fate determination. J Biol Chem 285 (2010).","DOI":"10.1074\/jbc.M110.139477"},{"key":"18700_CR12","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/j.jnutbio.2005.09.007","volume":"17","author":"K Nagai","year":"2006","unstructured":"Nagai, K., Chiba, A., Nishino, T., Kubota, T. & Kawagishi, H. Dilinoleoyl-phosphatidylethanolamine from Hericium erinaceum protects against ER stress-dependent Neuro2a cell death via protein kinase C pathway. J Nutr Biochem 17, 525\u2013530 (2006).","journal-title":"J Nutr Biochem"},{"key":"18700_CR13","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1038\/nature04598","volume":"440","author":"F Darios","year":"2006","unstructured":"Darios, F. & Davletov, B. Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Nature 440, 813\u2013817 (2006).","journal-title":"Nature"},{"key":"18700_CR14","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1038\/embor.2010.66","volume":"11","author":"F Darios","year":"2010","unstructured":"Darios, F. et al. Alpha-synuclein sequesters arachidonic acid to modulate SNARE-mediated exocytosis. EMBO Rep 11, 528\u2013533 (2010).","journal-title":"EMBO Rep"},{"key":"18700_CR15","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1073\/pnas.79.6.1698","volume":"79","author":"JD Esko","year":"1982","unstructured":"Esko, J. D., Nishijima, M. & Raetz, C. R. Animal cells dependent on exogenous phosphatidylcholine for membrane biogenesis. Proc Natl Acad Sci USA 79, 1698\u20131702 (1982).","journal-title":"Proc Natl Acad Sci USA"},{"key":"18700_CR16","doi-asserted-by":"crossref","first-page":"20","DOI":"10.3109\/10409238.2012.735643","volume":"48","author":"ND Ridgway","year":"2013","unstructured":"Ridgway, N. D. The role of phosphatidylcholine and choline metabolites to cell proliferation and survival. Crit Rev Biochem Mol Biol 48, 20\u201338 (2013).","journal-title":"Crit Rev Biochem Mol Biol"},{"key":"18700_CR17","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1038\/nbt.3626","volume":"34","author":"O Hilsenbeck","year":"2016","unstructured":"Hilsenbeck, O. et al. Software tools for single-cell tracking and quantification of cellular and molecular properties. Nat Biotechnol 34, 703\u2013706 (2016).","journal-title":"Nat Biotechnol"},{"key":"18700_CR18","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1242\/dev.009951","volume":"135","author":"MR Costa","year":"2008","unstructured":"Costa, M. R., Wen, G., Lepier, A., Schroeder, T. & Gotz, M. Par-complex proteins promote proliferative progenitor divisions in the developing mouse cerebral cortex. Development 135, 11\u201322 (2008).","journal-title":"Development"},{"key":"18700_CR19","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.neuron.2009.07.004","volume":"63","author":"RS Bultje","year":"2009","unstructured":"Bultje, R. S. et al. Mammalian Par3 regulates progenitor cell asymmetric division via notch signaling in the developing neocortex. Neuron 63, 189\u2013202 (2009).","journal-title":"Neuron"},{"key":"18700_CR20","doi-asserted-by":"crossref","unstructured":"Landeira, B. S. et al. Activity-Independent Effects of CREB on Neuronal Survival and Differentiation during Mouse Cerebral Cortex Development. Cereb Cortex (2016).","DOI":"10.1093\/cercor\/bhw387"},{"key":"18700_CR21","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0896-6273(00)00086-6","volume":"28","author":"X Qian","year":"2000","unstructured":"Qian, X. et al. Timing of CNS cell generation: a programmed sequence of neuron and glial cell production from isolated murine cortical stem cells. Neuron 28, 69\u201380 (2000).","journal-title":"Neuron"},{"key":"18700_CR22","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","volume":"65","author":"T Mosmann","year":"1983","unstructured":"Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65, 55\u201363 (1983).","journal-title":"J Immunol Methods"},{"key":"18700_CR23","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/j.neuron.2012.08.031","volume":"75","author":"X Li","year":"2012","unstructured":"Li, X. et al. MEK Is a Key Regulator of Gliogenesis in the Developing Brain. Neuron 75, 1035\u20131050 (2012).","journal-title":"Neuron"},{"key":"18700_CR24","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1111\/j.1365-2362.2008.01990.x","volume":"38","author":"N Farrokhnia","year":"2008","unstructured":"Farrokhnia, N., Ericsson, A., Terent, A. & Lennmyr, F. MEK-inhibitor U0126 in hyperglycaemic focal ischaemic brain injury in the rat. Eur J Clin Invest 38, 679\u2013685 (2008).","journal-title":"Eur J Clin Invest"},{"key":"18700_CR25","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1111\/iju.13386","volume":"24","author":"K Nakajima","year":"2017","unstructured":"Nakajima, K., Edenbrandt, L. & Mizokami, A. Bone scan index: A new biomarker of bone metastasis in patients with prostate cancer. Int J Urol 24, 668\u2013673 (2017).","journal-title":"Int J Urol"},{"key":"18700_CR26","doi-asserted-by":"crossref","unstructured":"Schmuck, S. et al. Initial Experience with Volumetric 68Ga-PSMA I&T PET\/CT for Assessment of Whole-body Tumor Burden as a Quantitative Imaging Biomarker in Patients with Prostate Cancer. J Nucl Med (2017).","DOI":"10.2967\/jnumed.117.193581"},{"key":"18700_CR27","doi-asserted-by":"crossref","first-page":"7966","DOI":"10.1523\/JNEUROSCI.1054-09.2009","volume":"29","author":"R Jagasia","year":"2009","unstructured":"Jagasia, R. et al. GABA-cAMP response element-binding protein signaling regulates maturation and survival of newly generated neurons in the adult hippocampus. J Neurosci 29, 7966\u20137977 (2009).","journal-title":"J Neurosci"},{"key":"18700_CR28","doi-asserted-by":"crossref","first-page":"2863","DOI":"10.1242\/dev.127.13.2863","volume":"127","author":"AR Desai","year":"2000","unstructured":"Desai, A. R. & McConnell, S. K. Progressive restriction in fate potential by neural progenitors during cerebral cortical development. Development 127, 2863\u20132872 (2000).","journal-title":"Development"},{"key":"18700_CR29","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.4103\/1673-5374.137570","volume":"9","author":"L Xiao","year":"2014","unstructured":"Xiao, L., Saiki, C. & Ide, R. Stem cell therapy for central nerve system injuries: glial cells hold the key. Neural Regen Res 9, 1253\u20131260 (2014).","journal-title":"Neural Regen Res"},{"issue":"Suppl 1","key":"18700_CR30","doi-asserted-by":"crossref","first-page":"i135","DOI":"10.1093\/cercor\/bhp046","volume":"19","author":"MR Costa","year":"2009","unstructured":"Costa, M. R., Bucholz, O., Schroeder, T. & Gotz, M. Late origin of glia-restricted progenitors in the developing mouse cerebral cortex. Cereb Cortex 19 (Suppl 1), i135\u2013143 (2009).","journal-title":"Cereb Cortex"},{"key":"18700_CR31","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1038\/nrn3618","volume":"14","author":"M Kohwi","year":"2013","unstructured":"Kohwi, M. & Doe, C. Q. Temporal fate specification and neural progenitor competence during development. Nat Rev Neurosci 14, 823\u2013838 (2013).","journal-title":"Nat Rev Neurosci"},{"key":"18700_CR32","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1523\/JNEUROSCI.4374-07.2008","volume":"28","author":"D Colak","year":"2008","unstructured":"Colak, D. et al. Adult neurogenesis requires Smad4-mediated bone morphogenic protein signaling in stem cells. J Neurosci 28, 434\u2013446 (2008).","journal-title":"J Neurosci"},{"key":"18700_CR33","doi-asserted-by":"crossref","first-page":"3303","DOI":"10.1242\/dev.093096","volume":"140","author":"EB Sequerra","year":"2013","unstructured":"Sequerra, E. B., Costa, M. R., Menezes, J. R. & Hedin-Pereira, C. Adult neural stem cells: plastic or restricted neuronal fates? Development 140, 3303\u20133309 (2013).","journal-title":"Development"},{"key":"18700_CR34","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.stem.2017.06.011","volume":"21","author":"S Gascon","year":"2017","unstructured":"Gascon, S., Masserdotti, G., Russo, G. L. & Gotz, M. Direct Neuronal Reprogramming: Achievements, Hurdles, and New Roads to Success. Cell Stem Cell 21, 18\u201334 (2017).","journal-title":"Cell Stem Cell"},{"key":"18700_CR35","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1038\/cr.2011.177","volume":"22","author":"AD Panopoulos","year":"2012","unstructured":"Panopoulos, A. D. et al. The metabolome of induced pluripotent stem cells reveals metabolic changes occurring in somatic cell reprogramming. Cell Res 22, 168\u2013177 (2012).","journal-title":"Cell Res"},{"key":"18700_CR36","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1126\/science.aaf4802","volume":"353","author":"N Urban","year":"2016","unstructured":"Urban, N. et al. Return to quiescence of mouse neural stem cells by degradation of a proactivation protein. Science 353, 292\u2013295 (2016).","journal-title":"Science"},{"key":"18700_CR37","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1126\/science.aab3516","volume":"349","author":"AT Guy","year":"2015","unstructured":"Guy, A. T. et al. Neuronal Development. Glycerophospholipid regulation of modality-specific sensory axon guidance in the spinal cord. Science 349, 974\u2013977 (2015).","journal-title":"Science"},{"key":"18700_CR38","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/S0169-328X(02)00550-8","volume":"109","author":"G Kim","year":"2002","unstructured":"Kim, G., Choe, Y., Park, J., Cho, S. & Kim, K. Activation of protein kinase A induces neuronal differentiation of HiB5 hippocampal progenitor cells. Brain Res Mol Brain Res 109, 134\u2013145 (2002).","journal-title":"Brain Res Mol Brain Res"},{"key":"18700_CR39","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.yexcr.2009.08.006","volume":"316","author":"G Lepski","year":"2010","unstructured":"Lepski, G. et al. Limited Ca2+ and PKA-pathway dependent neurogenic differentiation of human adult mesenchymal stem cells as compared to fetal neuronal stem cells. Exp Cell Res 316, 216\u2013231 (2010).","journal-title":"Exp Cell Res"},{"key":"18700_CR40","doi-asserted-by":"crossref","first-page":"5093","DOI":"10.1038\/sj.emboj.7601923","volume":"26","author":"S Seo","year":"2007","unstructured":"Seo, S., Lim, J. W., Yellajoshyula, D., Chang, L. W. & Kroll, K. L. Neurogenin and NeuroD direct transcriptional targets and their regulatory enhancers. EMBO J 26, 5093\u20135108 (2007).","journal-title":"EMBO J"},{"key":"18700_CR41","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1523\/JNEUROSCI.4568-12.2013","volume":"33","author":"A Sato","year":"2013","unstructured":"Sato, A. & Takeda, H. Neuronal subtypes are specified by the level of neurod expression in the zebrafish lateral line. J Neurosci 33, 556\u2013562 (2013).","journal-title":"J Neurosci"},{"key":"18700_CR42","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/43686","volume":"401","author":"K Yeung","year":"1999","unstructured":"Yeung, K. et al. Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP. Nature 401, 173\u2013177 (1999).","journal-title":"Nature"},{"key":"18700_CR43","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1517\/14728222.12.10.1275","volume":"12","author":"L Zeng","year":"2008","unstructured":"Zeng, L., Imamoto, A. & Rosner, M. R. Raf kinase inhibitory protein (RKIP): a physiological regulator and future therapeutic target. Expert Opin Ther Targets 12, 1275\u20131287 (2008).","journal-title":"Expert Opin Ther Targets"},{"key":"18700_CR44","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s12031-015-0612-8","volume":"57","author":"J Wei","year":"2015","unstructured":"Wei, J., Jiang, H., Gao, H. & Wang, G. Raf-1 Kinase Inhibitory Protein (RKIP) Promotes Retinal Ganglion Cell Survival and Axonal Regeneration Following Optic Nerve Crush. J Mol Neurosci 57, 243\u2013248 (2015).","journal-title":"J Mol Neurosci"},{"key":"18700_CR45","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.cellsig.2015.11.016","volume":"28","author":"I Fujimoto","year":"2016","unstructured":"Fujimoto, I., Hasegawa, K., Fujiwara, K., Yamada, M. & Yoshikawa, K. Necdin controls EGFR signaling linked to astrocyte differentiation in primary cortical progenitor cells. Cell Signal 28, 94\u2013107 (2016).","journal-title":"Cell Signal"},{"key":"18700_CR46","doi-asserted-by":"crossref","first-page":"2159","DOI":"10.3727\/096368911X627543","volume":"21","author":"T Toyoda","year":"2012","unstructured":"Toyoda, T. et al. Suppression of astrocyte lineage in adult hippocampal progenitor cells expressing hippocampal cholinergic neurostimulating Peptide precursor in an in vivo ischemic model. Cell Transplant 21, 2159\u20132169 (2012).","journal-title":"Cell Transplant"},{"key":"18700_CR47","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.brainres.2010.02.071","volume":"1327","author":"T Sagisaka","year":"2010","unstructured":"Sagisaka, T. et al. Directed neural lineage differentiation of adult hippocampal progenitor cells via modulation of hippocampal cholinergic neurostimulating peptide precursor expression. Brain Res 1327, 107\u2013117 (2010).","journal-title":"Brain Res"},{"key":"18700_CR48","doi-asserted-by":"crossref","first-page":"2706","DOI":"10.1152\/jn.00509.2016","volume":"116","author":"E Wanke","year":"2016","unstructured":"Wanke, E., Gullo, F., Dossi, E., Valenza, G. & Becchetti, A. Neuron-glia cross talk revealed in reverberating networks by simultaneous extracellular recording of spikes and astrocytes\u2019 glutamate transporter and K+ currents. J Neurophysiol 116, 2706\u20132719 (2016).","journal-title":"J Neurophysiol"},{"key":"18700_CR49","doi-asserted-by":"crossref","first-page":"119","DOI":"10.3389\/fphys.2012.00119","volume":"3","author":"C Fruhbeis","year":"2012","unstructured":"Fruhbeis, C., Frohlich, D. & Kramer-Albers, E. M. Emerging roles of exosomes in neuron-glia communication. Front Physiol 3, 119 (2012).","journal-title":"Front Physiol"},{"key":"18700_CR50","first-page":"501","volume":"9","author":"LF Batiz","year":"2015","unstructured":"Batiz, L. F. et al. Exosomes as Novel Regulators of Adult Neurogenic Niches. Front Cell Neurosci 9, 501 (2015).","journal-title":"Front Cell Neurosci"},{"key":"18700_CR51","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.taap.2015.06.003","volume":"287","author":"MR Arana","year":"2015","unstructured":"Arana, M. R. et al. Coordinated induction of GST and MRP2 by cAMP in Caco-2 cells: Role of protein kinase A signaling pathway and toxicological relevance. Toxicol Appl Pharmacol 287, 178\u2013190 (2015).","journal-title":"Toxicol Appl Pharmacol"},{"key":"18700_CR52","doi-asserted-by":"crossref","unstructured":"Chojnacka, A., Gladkowski, W. & Grudniewska, A. Lipase-Catalyzed Transesterification of Egg-Yolk Phophatidylcholine with Concentrate of n-3 Polyunsaturated Fatty Acids from Cod Liver Oil. Molecules 22 (2017).","DOI":"10.3390\/molecules22101771"},{"key":"18700_CR53","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.foodchem.2016.10.043","volume":"221","author":"AH Ali","year":"2017","unstructured":"Ali, A. H. et al. Identification of phospholipids classes and molecular species in different types of egg yolk by using UPLC-Q-TOF-MS. Food Chem 221, 58\u201366 (2017).","journal-title":"Food Chem"},{"key":"18700_CR54","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0076-6879(06)19001-1","volume":"419","author":"RL Rietze","year":"2006","unstructured":"Rietze, R. L. & Reynolds, B. A. Neural stem cell isolation and characterization. Methods Enzymol 419, 3\u201323 (2006).","journal-title":"Methods Enzymol"},{"key":"18700_CR55","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/0003-2697(77)90428-6","volume":"79","author":"JJ Sedmak","year":"1977","unstructured":"Sedmak, J. J. & Grossberg, S. E. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250. Anal Biochem 79, 544\u2013552 (1977).","journal-title":"Anal Biochem"},{"key":"18700_CR56","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1242\/dev.061663","volume":"138","author":"MR Costa","year":"2011","unstructured":"Costa, M. R. et al. Continuous live imaging of adult neural stem cell division and lineage progression in vitro. Development 138, 1057\u20131068 (2011).","journal-title":"Development"}],"updated-by":[{"DOI":"10.1038\/s41598-019-55787-3","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2019,12,24]],"date-time":"2019-12-24T00:00:00Z","timestamp":1577145600000}}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-18700-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-18700-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-18700-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T00:45:00Z","timestamp":1671583500000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-18700-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,11]]},"references-count":56,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2018,12]]}},"alternative-id":["18700"],"URL":"https:\/\/doi.org\/10.1038\/s41598-017-18700-4","relation":{"correction":[{"id-type":"doi","id":"10.1038\/s41598-019-55787-3","asserted-by":"object"}]},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,11]]},"assertion":[{"value":"18 September 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 December 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 January 2018","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 December 2019","order":4,"name":"change_date","label":"Change Date","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Correction","order":5,"name":"change_type","label":"Change Type","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"An amendment to this paper has been published and can be accessed via a link at the top of the paper.","order":6,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"460"}}