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However, efficient treatment options to reduce amyloid fibrils\n                    <jats:italic>in vivo<\/jats:italic>\n                    are still lacking. Therefore, we tested the effect of epigallocatechin gallate (EGCG) on fibril formation\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    . To determine the binding of hIAPP and EGCG,\n                    <jats:italic>in vitro<\/jats:italic>\n                    interaction studies were performed. To inhibit amyloid plaque formation\n                    <jats:italic>in vivo<\/jats:italic>\n                    , homozygous (tg\/tg), hemizygous (wt\/tg), and control mice (wt\/wt) were treated with EGCG. EGCG bound to hIAPP\n                    <jats:italic>in vitro<\/jats:italic>\n                    and induced formation of amorphous aggregates instead of amyloid fibrils. Amyloid fibrils were detected in the pancreatic islets of tg\/tg mice, which was associated with disrupted islet structure and diabetes. Although pancreatic amyloid fibrils could be detected in wt\/tg mice, these animals were non-diabetic. EGCG application decreased amyloid fibril intensity in wt\/tg mice, however it was ineffective in tg\/tg animals. Our data indicate that EGCG inhibits amyloid fibril formation\n                    <jats:italic>in vitro<\/jats:italic>\n                    and reduces fibril intensity in non-diabetic wt\/tg mice. These results demonstrate a possible\n                    <jats:italic>in vivo<\/jats:italic>\n                    effectiveness of EGCG on amyloid formation and suggest an early therapeutical application.\n                  <\/jats:p>","DOI":"10.1038\/s41598-017-18807-8","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T07:36:51Z","timestamp":1515742611000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":67,"title":["Epigallocatechin gallate (EGCG) reduces the intensity of pancreatic amyloid fibrils in human islet amyloid polypeptide (hIAPP) transgenic mice"],"prefix":"10.1038","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0485-5439","authenticated-orcid":false,"given":"Andras","family":"Franko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana C.","family":"Rodriguez Camargo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annett","family":"B\u00f6ddrich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Divita","family":"Garg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andres","family":"Rodriguez Camargo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"Rathkolb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Janik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Aichler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Feuchtinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frauke","family":"Neff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5143-2677","authenticated-orcid":false,"given":"Helmut","family":"Fuchs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erich E.","family":"Wanker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd","family":"Reif","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Ulrich","family":"H\u00e4ring","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Peter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7898-2353","authenticated-orcid":false,"given":"Martin","family":"Hrab\u011b de Angelis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,1,18]]},"reference":[{"key":"18807_CR1","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1007\/s13311-013-0192-7","volume":"10","author":"J Bieschke","year":"2013","unstructured":"Bieschke, J. 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