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Activation of \u00b5-opioid receptors in the nucleus tractus solitarius (NTS) increases blood pressure. Electroacupuncture (EA) stimulation of somatosensory nerves underneath acupoints P5\u20136, ST36\u201337, LI6\u20137 or G37\u201339 selectively but differentially\u00a0modulates sympathoexcitatory responses. We therefore\u00a0hypothesized that EA-stimulation at P5\u20136 or ST36\u201337, but not LI6\u20137 or G37\u201339 acupoints, inhibits the bradycardia and vasodepression through a \u00b5-opioid receptor mechanism in the NTS. We observed that stimulation at acupoints P5\u20136 and ST36\u201337 overlying the deep somatosensory nerves and LI6\u20137 and G37\u201339 overlying cutaneous nerves differentially evoked NTS neural activity in anesthetized and ventilated animals. Thirty-min of EA-stimulation at P5\u20136 or ST36\u201337 reduced the depressor and bradycardia responses to PBG while EA at LI6\u20137 or G37\u201339 did not. Congruent with the hemodynamic responses, EA at P5\u20136 and ST36\u201337, but not at LI6\u20137 and G37\u201339, reduced vagally evoked activity of cardiovascular NTS cells. Finally, opioid receptor blockade in the NTS with naloxone or a specific\u00a0\u03bc-receptor antagonist reversed P5\u20136 EA-inhibition of the depressor, bradycardia and vagally evoked NTS activity. These data suggest that point specific EA stimulation inhibits PBG-induced vasodepression and bradycardia responses through a \u03bc-opioid mechanism in the NTS.<\/jats:p>","DOI":"10.1038\/s41598-018-19672-9","type":"journal-article","created":{"date-parts":[[2018,1,24]],"date-time":"2018-01-24T15:08:48Z","timestamp":1516806528000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Modulation of Neurally Mediated Vasodepression and Bradycardia by Electroacupuncture through Opioids in Nucleus Tractus Solitarius"],"prefix":"10.1038","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8808-7224","authenticated-orcid":false,"given":"Stephanie C.","family":"Tjen-A-Looi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang-Wu","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Ling","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Longhurst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,1,30]]},"reference":[{"key":"19672_CR1","doi-asserted-by":"publisher","first-page":"1313","DOI":"10.1152\/ajpregu.00300.2014","volume":"307","author":"SC Tjen-A-Looi","year":"2014","unstructured":"Tjen-A-Looi, S. 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