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Here, we report that the anillin-related protein Int1 interacts with septins for coupling cytokinesis with nuclear segregation. Loss of Int1 results in a rapid disassembly of duplicated septin rings from the bud neck at the onset of actomyosin ring contraction. Strikingly, this has no major impact on cytokinesis and septum formation. However, Int1 genetically interacts with the Sep7 septin, maintaining the diffusion barrier at the bud neck and guarantying a faithful nuclear segregation. Indeed, <jats:italic>int1<\/jats:italic>\u0394\u0394 <jats:italic>sep7<\/jats:italic>\u0394\u0394 mutant cells, in contrast to <jats:italic>int1<\/jats:italic>\u0394\u0394 <jats:italic>cdc10<\/jats:italic>\u0394\u0394, undergo a premature activation of mitotic exit prior to the alignment of the mitotic spindle with the division axis, producing large multinucleated cells. Some of these multinucleated cells arise from trimeras similar to those observed upon fluconazole exposure. Finally, the defects in nuclear segregation could be in part due to the inability to maintain the Lte1 mitotic exit activator at the cortex of the daughter cell. These results suggest that Int1 and Sep7 play a role in maintaining genome stability by acting as a diffusion barrier for Lte1.<\/jats:p>","DOI":"10.1038\/s41598-018-20249-9","type":"journal-article","created":{"date-parts":[[2018,1,29]],"date-time":"2018-01-29T12:34:46Z","timestamp":1517229286000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The anillin-related Int1 protein and the Sep7 septin collaborate to maintain cellular ploidy in Candida albicans"],"prefix":"10.1038","volume":"8","author":[{"given":"Sara","family":"Orellana-Mu\u00f1oz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Encarnaci\u00f3n","family":"Due\u00f1as-Santero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yolanda","family":"Arn\u00e1iz-Pita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"del Rey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaime","family":"Correa-Bordes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos R.","family":"V\u00e1zquez de Aldana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,2,2]]},"reference":[{"key":"20249_CR1","doi-asserted-by":"publisher","first-page":"737","DOI":"10.1534\/genetics.108.090878","volume":"179","author":"EM Torres","year":"2008","unstructured":"Torres, E. 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