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Modified-live virus (MLV) vaccines are used to control PRRS in swine herds. However, immunized sows and piglets often generate variable antibody levels. This study aimed to detect significant genes and pathways involved in antibody responsiveness of pregnant sows and their offspring post-PRRSV vaccination. RNA sequencing was conducted on peripheral blood-mononuclear cells (PBMCs), which were isolated from pregnant sows and their piglets with high (HA), median (MA), and low (LA) PRRS antibody levels following vaccination. 401 differentially expressed genes (DEGs) were identified in three comparisons (HA<jats:italic>versus<\/jats:italic>MA, HA<jats:italic>versus<\/jats:italic>LA, and MA<jats:italic>versus<\/jats:italic>LA) of sow PBMCs. Two novel pathways (complement and coagulation cascade pathway; and epithelial cell signaling in<jats:italic>H. pylori<\/jats:italic>infection pathway) revealed by DEGs in HA<jats:italic>versus<\/jats:italic>LA and MA<jats:italic>versus<\/jats:italic>LA were involved in chemotactic and proinflammatory responses.<jats:italic>TNF-\u03b1<\/jats:italic>,<jats:italic>CCL4<\/jats:italic>, and<jats:italic>NFKBIA<\/jats:italic>genes displayed the same expression trends in subsequent generation post-PRRS-MLV vaccination. Findings of the study suggest that two pathways and<jats:italic>TNF-\u03b1<\/jats:italic>,<jats:italic>CCL4<\/jats:italic>, and<jats:italic>NFKBIA<\/jats:italic>could be considered as key pathways and potential candidate genes for PRRSV vaccine responsiveness, respectively.<\/jats:p>","DOI":"10.1038\/s41598-018-20701-w","type":"journal-article","created":{"date-parts":[[2018,1,31]],"date-time":"2018-01-31T10:10:49Z","timestamp":1517393449000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Transcriptome of Porcine PBMCs over Two Generations Reveals Key Genes and Pathways Associated with Variable Antibody Responses post PRRSV Vaccination"],"prefix":"10.1038","volume":"8","author":[{"given":"Ting","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengxia","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liwei","family":"Zhai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiyong","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangyang","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Ning","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiliang","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Ao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuduan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,2,6]]},"reference":[{"key":"20701_CR1","doi-asserted-by":"publisher","first-page":"385","DOI":"10.2460\/javma.2005.227.385","volume":"227","author":"EJ Neumann","year":"2005","unstructured":"Neumann, E. 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