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For some isolates, phenotypic tropism testing (PTT) revealed discordant results with GTT. In this study, we performed a comparative study between GTT and PTT in HIV-1C from East Africa (HIV-1C<jats:sub>EA<\/jats:sub>) and compared the data with HIV-1B and 01_AE and described the maraviroc susceptibility in the CCR5-tropic strains. Patient-derived HIV-1 envgp120 region was cloned into a modified pNL4-3 plasmid expressing the luciferase gene. rPhenotyping dissected single clones from 31 HIV-1C<jats:sub>EA<\/jats:sub> infected patients and four strains with known phenotype. Additionally, 68 clones from 18 patients (HIV-1B: 5, 01_AE: 7, HIV-1C<jats:sub>EA<\/jats:sub>: 6) were used to determine the PTT in GHOST cell line. The respective V3-sequences were used for GTT. R5-tropic strains from HIV-1C<jats:sub>EA<\/jats:sub> (n\u2009=\u200920) and non-C (n\u2009=\u200912) were tested for maraviroc sensitivity in TZMbl cell line. The GTT falsely called a higher proportion of X4-tropic strains in HIV-1C<jats:sub>ET<\/jats:sub> compared to PTT by both rPhenotyping and the GHOST-cell assay. When multiple clones were tested in a subset of patients\u2019 samples, both dual-tropic and R5-tropic strains were identified for HIV-1C. Relatively higher EC<jats:sub>50<\/jats:sub> values were observed in HIV-1C strains than the non-C strains (p\u2009=\u20090.002).<\/jats:p>","DOI":"10.1038\/s41598-018-20814-2","type":"journal-article","created":{"date-parts":[[2018,1,30]],"date-time":"2018-01-30T11:12:28Z","timestamp":1517310748000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Phenotypic co-receptor tropism and Maraviroc sensitivity in HIV-1 subtype C from East Africa"],"prefix":"10.1038","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0037-7679","authenticated-orcid":false,"given":"Abu Bakar","family":"Siddik","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Haas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md Shanawazur","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shambhu Ganeshappa","family":"Aralaguppe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wondwossen","family":"Amogne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joelle","family":"Bader","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Klimkait","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0844-3338","authenticated-orcid":false,"given":"Ujjwal","family":"Neogi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,2,5]]},"reference":[{"key":"20814_CR1","doi-asserted-by":"publisher","DOI":"10.1038\/srep08543","volume":"5","author":"K Cashin","year":"2015","unstructured":"Cashin, K. et al. 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