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A total of 52 samples were collected from recreational lakes in Tianjin during a high-occurrence season (June\u2013October) for the waterborne cryptosporidiosis and giardiasis, and the occurrence and genotypes of <jats:italic>Cryptosporidium<\/jats:italic> and <jats:italic>Giardia<\/jats:italic> were investigated. The results showed that 82.7% (43) and 98.1% (51) of samples were positive for <jats:italic>Cryptosporidium<\/jats:italic> oocyst and <jats:italic>Giardia<\/jats:italic> cysts, respectively. The mean concentration of parasites was 3.65 oocysts\/10\u2009L and 12.58 cysts\/10\u2009L, respectively. Molecular characterization revealed that the presence of <jats:italic>Cryptosporidium parvum<\/jats:italic>, <jats:italic>C. andersoni<\/jats:italic>, <jats:italic>C. hominis<\/jats:italic>, <jats:italic>C. meleagridis<\/jats:italic>, <jats:italic>C. fragile<\/jats:italic>, <jats:italic>C. ubiquitum<\/jats:italic>, and <jats:italic>Giardia lamblia<\/jats:italic> assemblage A, B and D. The protozoan contamination in the studied lakes may originate from animal feces on ground, which was washed into the lake by stormwater runoff. Nevertheless, there is a potential risk of infection during recreational activities in the lake because the dominant detected protozoan genotypes are common human pathogens. Moreover, microbial indicators analysis does not adequately indicate the protozoan contamination in recreational water. The information from this study will be valuable for future protozoan source tracking, and any further control interventions against <jats:italic>Cryptosporidium<\/jats:italic> and\/or <jats:italic>Giardia<\/jats:italic> infection associated with recreational water.<\/jats:p>","DOI":"10.1038\/s41598-018-20902-3","type":"journal-article","created":{"date-parts":[[2018,1,30]],"date-time":"2018-01-30T10:46:56Z","timestamp":1517309216000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Presence and molecular characterization of Cryptosporidium and Giardia in recreational lake water in Tianjin, China: a preliminary study"],"prefix":"10.1038","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1688-6200","authenticated-orcid":false,"given":"Shumin","family":"Xiao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sike","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,2,5]]},"reference":[{"key":"20902_CR1","first-page":"554","volume":"95","author":"A Perkins","year":"2017","unstructured":"Perkins, A. & Trimmier, M. 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