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A vaccine against <jats:italic>S<\/jats:italic>. <jats:italic>brasiliensis<\/jats:italic> could improve the current sporotrichosis situation. Here, we show 3 peptides from <jats:italic>S<\/jats:italic>. <jats:italic>brasiliensis<\/jats:italic> immunogenic proteins that have a higher likelihood for engaging MHC-class II molecules. We investigated the efficiency of the peptides as vaccines for preventing subcutaneous sporotrichosis. In this study, we observed a decrease in lesion diameters in peptide-immunized mice, showing that the peptides could induce a protective immune response against subcutaneous sporotrichosis. ZR8 peptide is from the GP70 protein, the main antigen of the <jats:italic>Sporothrix<\/jats:italic> complex, and was the best potential vaccine candidate by increasing CD4<jats:sup>+<\/jats:sup> T cells and higher levels of IFN-\u03b3, IL-17A and IL-1\u03b2 characterizing a strong cellular immune response. This immune environment induced a higher number of neutrophils in lesions that are associated with fungus clearance. These results indicated that the ZR8 peptide induces a protective immune response against subcutaneous sporotrichosis and is a vaccine candidate against <jats:italic>S<\/jats:italic>. <jats:italic>brasiliensis<\/jats:italic> infection.<\/jats:p>","DOI":"10.1038\/s41598-018-22709-8","type":"journal-article","created":{"date-parts":[[2018,3,2]],"date-time":"2018-03-02T12:40:41Z","timestamp":1519994441000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["An immunoproteomic approach revealing peptides from Sporothrix brasiliensis that induce a cellular immune response in subcutaneous sporotrichosis"],"prefix":"10.1038","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9470-4695","authenticated-orcid":false,"given":"Jos\u00e9 Roberto Foga\u00e7a","family":"de Almeida","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grasielle Pereira","family":"Jannuzzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilberto Hideo","family":"Kaihami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandro Carvalho Dantas","family":"Breda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen Spadari","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandro Rog\u00e9rio","family":"de Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,3,8]]},"reference":[{"key":"22709_CR1","doi-asserted-by":"publisher","first-page":"3198","DOI":"10.1128\/JCM.00808-07","volume":"45","author":"R Marimon","year":"2007","unstructured":"Marimon, R. et al. 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