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Although both are Gram-negative bacteria, the SERS signatures of <jats:italic>C<\/jats:italic>. <jats:italic>trachomatis<\/jats:italic> and <jats:italic>N<\/jats:italic>. <jats:italic>gonorrheoae<\/jats:italic> are completely different. <jats:italic>N<\/jats:italic>. <jats:italic>gonorrheoae<\/jats:italic> SERS spectra are due to the starvation induced nucleotide metabolites adenine and guanine, and the surface associated co-enzyme nicotinamide adenine dinucleotide and are very similar on Au and Ag although the spectrum appears more rapidly on Ag. The <jats:italic>C<\/jats:italic>. <jats:italic>trachomatis<\/jats:italic> SERS spectrum is dominated by the vibrational features of cell surface proteins. While features attributable to specific residues and the amide backbone characterize the <jats:italic>C<\/jats:italic>. <jats:italic>trachomatis<\/jats:italic> spectrum on Ag, the corresponding SERS spectrum on Au substrates displays vibrational characteristics of aggregated proteins. The prospects for the development of a SERS based platform for rapid (&lt;one hour), low-cost bacterial STD diagnostics are promising based on these initial studies. Furthermore, this biomedical application demonstrates the potential for SERS to be a sensitive real time probe of the dynamics of biochemical activity in the cell wall and extracellular regions of microorganisms.<\/jats:p>","DOI":"10.1038\/s41598-018-23562-5","type":"journal-article","created":{"date-parts":[[2018,3,29]],"date-time":"2018-03-29T01:51:11Z","timestamp":1522288271000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Surface enhanced Raman spectroscopy of Chlamydia trachomatis and Neisseria gonorrhoeae for diagnostics, and extra-cellular metabolomics and biochemical monitoring"],"prefix":"10.1038","volume":"8","author":[{"given":"Y.","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. R.","family":"Premasiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. 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