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The \u03b1 and \u03b2 isoforms are largely brain-specific, where they mediate synaptic functions underlying learning, memory and cognition. Here, we determined the \u03b1 and \u03b2 splice-variant distribution among different mouse brain regions. Surprisingly, the nuclear variant \u03b1B was detected in all regions, and even dominated in hypothalamus and brain stem. For CaMKII\u03b2, the full-length variant dominated in most regions (with higher amounts of minor variants again seen in hypothalamus and brain stem). The mammalian but not fish CaMKII\u03b2 gene lacks exon v3\n                    <jats:sub>N<\/jats:sub>\n                    that encodes the nuclear localization signal in \u03b1\n                    <jats:sub>B<\/jats:sub>\n                    , but contains three exons not found in the CaMKII\u03b1 gene (exons v1, v4, v5). While skipping of exons v1 and\/or v5 generated the minor splice-variants \u03b2\u2019, \u03b2e and \u03b2e\u2019, essentially all transcripts contained exon v4. However, we instead detected another minor splice-variant (now termed \u03b2H), which lacks part of the hub domain that mediates formation of CaMKII holoenzymes. Surprisingly, in an optogenetic cellular assay of protein interactions, CaMKII\u03b2H was impaired for binding to the \u03b2 hub domain, but still bound CaMKII\u03b1. This provides the first indication for isoform-specific differences in holoenzyme formation.\n                  <\/jats:p>","DOI":"10.1038\/s41598-018-23779-4","type":"journal-article","created":{"date-parts":[[2018,3,28]],"date-time":"2018-03-28T22:17:38Z","timestamp":1522275458000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Analysis of the CaMKII\u03b1 and \u03b2 splice-variant distribution among brain regions reveals isoform-specific differences in holoenzyme formation"],"prefix":"10.1038","volume":"8","author":[{"given":"Sarah G.","family":"Cook","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley M.","family":"Bourke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"O\u2019Leary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Zaegel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Lasda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janna","family":"Mize-Berge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidia","family":"Quillinan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandra L.","family":"Tucker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven J.","family":"Coultrap","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paco S.","family":"Herson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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