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Blocking lead uptake in the gastrointestinal tract is a novel prevention strategy. Whole-cell biosorbent for lead was constructed with PbrR genetically engineered on the cell surface of <jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic>), a predominant strain among intestinal microflora, using lipoprotein (Lpp)-OmpA as the anchoring protein. <jats:italic>In vitro<\/jats:italic>, the PbrR displayed cells had an enhanced ability for immobilizing toxic lead(II) ions from the external media at both acidic and neutral pH, and exhibited a higher specific adsorption for lead compared to other physiological two valence metal ions. <jats:italic>In vivo<\/jats:italic>, the persistence of recombinant <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> in the murine intestinal tract and the integrity of surface displayed PbrR were confirmed. In addition, oral administration of surface-engineered <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> was safe in mice, in which the concentrations of physiological metal ions in blood were not affected. More importantly, lead associated with PbrR-displayed <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> was demonstrated to be less bioavailable in the experimental mouse model with exposure to oral lead. This is reflected by significantly lower blood and femur lead concentrations in PbrR-displayed <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> groups compared to the control. These results open up the possibility for the removal of toxic metal ions <jats:italic>in vivo<\/jats:italic> using engineered microorganisms as adsorbents.<\/jats:p>","DOI":"10.1038\/s41598-018-24134-3","type":"journal-article","created":{"date-parts":[[2018,4,4]],"date-time":"2018-04-04T03:25:41Z","timestamp":1522812341000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice"],"prefix":"10.1038","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0958-1986","authenticated-orcid":false,"given":"Changye","family":"Hui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoxian","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Limei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianqing","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,4,9]]},"reference":[{"key":"24134_CR1","first-page":"130","volume":"76","author":"J Liu","year":"2014","unstructured":"Liu, J. & Lewis, G. 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