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However, presently there are no studies investigating the role of <jats:italic>EAAC1<\/jats:italic> in hippocampal neurogenesis. In this study, we tested the hypothesis that reduced cysteine transport into neurons by <jats:italic>EAAC1<\/jats:italic> knockout negatively affects adult hippocampal neurogenesis under physiological or pathological states. This study used young mice (aged 3\u20135 months) and aged mice (aged 11\u201315 months) of either the wild-type (WT) or <jats:italic>EAAC1<\/jats:italic><jats:sup>\n                  <jats:italic>\u2212\/\u2212<\/jats:italic>\n                <\/jats:sup> genotype. Ischemia was induced through the occlusion of bilateral common carotid arteries for 30\u2009minutes. Histological analysis was performed at 7 or 30 days after ischemia. We found that both young and aged mice with loss of the <jats:italic>EAAC1<\/jats:italic> displayed unaltered cell proliferation and neuronal differentiation, as compared to age-matched WT mice under ischemia-free conditions. However, neurons generated from <jats:italic>EAAC1<\/jats:italic><jats:sup>\n                  <jats:italic>\u2212\/\u2212<\/jats:italic>\n                <\/jats:sup> mice showed poor survival outcomes in both young and aged mice. In addition, deletion of <jats:italic>EAAC1<\/jats:italic> reduced the overall level of neurogenesis, including cell proliferation, differentiation, and survival after ischemia. The present study demonstrates that <jats:italic>EAAC1<\/jats:italic> is important for the survival of newly generated neurons in the adult brain under physiological and pathological conditions. Therefore, this study suggests that <jats:italic>EAAC1<\/jats:italic> plays an essential role in modulating hippocampal neurogenesis.<\/jats:p>","DOI":"10.1038\/s41598-018-25191-4","type":"journal-article","created":{"date-parts":[[2018,4,26]],"date-time":"2018-04-26T10:36:35Z","timestamp":1524738995000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["EAAC1 gene deletion reduces adult hippocampal neurogenesis after transient cerebral ischemia"],"prefix":"10.1038","volume":"8","author":[{"given":"Bo Young","family":"Choi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seok Joon","family":"Won","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Hee","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Sohn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong Ki","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae Nyoung","family":"Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae Yul","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7492-579X","authenticated-orcid":false,"given":"Sang Won","family":"Suh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,5,2]]},"reference":[{"key":"25191_CR1","doi-asserted-by":"publisher","first-page":"1030","DOI":"10.1038\/4151030a","volume":"415","author":"H van Praag","year":"2002","unstructured":"van Praag, H. et al. 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