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However, there has been relatively limited ability of this method to quantify the subtle changes that occur in the early stages of cartilage degeneration. An established mechanical indenting technique that has previously been used to examine the microstructural response of articular cartilage was employed to fix the bovine samples in an indented state. The samples were subject to creep loading with a constant compressive stress of 4.5 MPa and, when imaged using PS-OCT, enabled birefringent banding patterns to be observed. The magnitude of the birefringence was quantified using the birefringence coefficient (BRC) and statistical analysis revealed that PS-OCT is able to detect and quantify significant changes between healthy and early osteoarthritic cartilage (p &lt; 0.001). This presents a novel utilization of PS-OCT for future development as an <jats:italic>in vivo<\/jats:italic> assessment tool.<\/jats:p>","DOI":"10.1038\/s41598-018-25982-9","type":"journal-article","created":{"date-parts":[[2018,5,30]],"date-time":"2018-05-30T08:53:12Z","timestamp":1527670392000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Quantifying birefringence in the bovine model of early osteoarthritis using polarisation-sensitive optical coherence tomography and mechanical indentation"],"prefix":"10.1038","volume":"8","author":[{"given":"Matthew","family":"Goodwin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bastian","family":"Br\u00e4uer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Lewis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashvin","family":"Thambyah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9653-6907","authenticated-orcid":false,"given":"Fr\u00e9d\u00e9rique","family":"Vanholsbeeck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,6,5]]},"reference":[{"key":"25982_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.2165\/00003495-199600523-00003","volume":"52","author":"G Balint","year":"1996","unstructured":"Balint, G. & Szebenyi, B. 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