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We extended strength-duration curves for excitation of cardiomyocytes down to 200\u2009ns, and compared electroporative damage by proportionally more intense shocks of different duration. Enzymatically isolated murine, rabbit, and swine adult ventricular cardiomyocytes (VCM) were loaded with a Ca<jats:sup>2+<\/jats:sup> indicator Fluo-4 or Fluo-5N and subjected to shocks of increasing amplitude until a Ca<jats:sup>2+<\/jats:sup> transient was optically detected. Then, the voltage was increased 5-fold, and the electric cell injury was quantified by the uptake of a membrane permeability marker dye, propidium iodide. We established that: (1) Stimuli down to 200-ns duration can elicit Ca<jats:sup>2+<\/jats:sup> transients, although repeated ns shocks often evoke abnormal responses, (2) Stimulation thresholds expectedly increase as the shock duration decreases, similarly for VCMs from different species, (3) Stimulation threshold energy is minimal for the shortest shocks, (4) VCM orientation with respect to the electric field does not affect the threshold for ns shocks, and (5) The shortest shocks cause the least electroporation injury. These findings support further exploration of ns defibrillation, although abnormal response patterns to repetitive ns stimuli are of a concern and require mechanistic analysis.<\/jats:p>","DOI":"10.1038\/s41598-018-26521-2","type":"journal-article","created":{"date-parts":[[2018,5,22]],"date-time":"2018-05-22T15:22:09Z","timestamp":1527002529000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks"],"prefix":"10.1038","volume":"8","author":[{"given":"Iurii","family":"Semenov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey","family":"Grigoryev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna U.","family":"Neuber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian W.","family":"Zemlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga N.","family":"Pakhomova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maura","family":"Casciola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei G.","family":"Pakhomov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,5,29]]},"reference":[{"key":"26521_CR1","doi-asserted-by":"publisher","first-page":"1493","DOI":"10.1096\/fj.02-0859fje","volume":"17","author":"SJ Beebe","year":"2003","unstructured":"Beebe, S. 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