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A previous whole-genome analysis on <jats:italic>V. cholerae<\/jats:italic> O1 El Tor strains from the 2010 epidemic in Pakistan showed that all strains contained the <jats:italic>V. cholerae<\/jats:italic> pathogenicity island-1 and the accessory colonisation gene <jats:italic>acfC<\/jats:italic> (VC_0841). Here we show that <jats:italic>acfC<\/jats:italic> possess an open reading frame of 770\u2009bp encoding a protein with a predicted size of 28\u2009kDa, which shares high amino acid similarity with two adhesion proteins found in other enteropathogens, including Paa in serotype O45 porcine enteropathogenic <jats:italic>Escherichia coli<\/jats:italic> and PEB3 in <jats:italic>Campylobacter jejuni<\/jats:italic>. Using a defined <jats:italic>acfC<\/jats:italic> deletion mutant, we studied the specific role of AcfC in <jats:italic>V. cholerae<\/jats:italic> O1 El Tor environmental survival, colonisation and virulence in two infection model systems (<jats:italic>Galleria mellonella<\/jats:italic> and infant rabbits). Our results indicate that AcfC might be a periplasmic sulfate-binding protein that affects chemotaxis towards mucin and bacterial infectivity in the infant rabbit model of cholera. Overall, our findings suggest that AcfC contributes to the chemotactic response of WT <jats:italic>V. cholerae<\/jats:italic> and plays an important role in defining the overall distribution of the organism within the intestine.<\/jats:p>","DOI":"10.1038\/s41598-018-26570-7","type":"journal-article","created":{"date-parts":[[2018,5,24]],"date-time":"2018-05-24T11:04:58Z","timestamp":1527159898000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Vibrio cholerae accessory colonisation factor AcfC: a chemotactic protein with a role in hyperinfectivity"],"prefix":"10.1038","volume":"8","author":[{"given":"Esmeralda","family":"Valiente","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cadi","family":"Davies","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominic C.","family":"Mills","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2937-3420","authenticated-orcid":false,"given":"Maria","family":"Getino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer M.","family":"Ritchie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6140-9489","authenticated-orcid":false,"given":"Brendan W.","family":"Wren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,5,30]]},"reference":[{"key":"26570_CR1","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1056\/NEJMoa1012928","volume":"364","author":"CS Chin","year":"2011","unstructured":"Chin, C. 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