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In the current study, we used whole-genome sequencing to investigate the diversity of <jats:italic>bla<\/jats:italic><jats:sub>KPC<\/jats:sub>-containing plasmids and antimicrobial resistance mechanisms among 26 <jats:italic>bla<\/jats:italic><jats:sub>KPC<\/jats:sub>-containing <jats:italic>Escherichia coli<\/jats:italic>, and 13 <jats:italic>bla<\/jats:italic><jats:sub>KPC<\/jats:sub>-containing <jats:italic>Enterobacter asburiae, Enterobacter hormaechei<\/jats:italic>, <jats:italic>K. pneumoniae, Klebsiella variicola<\/jats:italic>, <jats:italic>Klebsiella michiganensis<\/jats:italic>, and <jats:italic>Serratia marcescens<\/jats:italic> strains, which were isolated from the same patients as the <jats:italic>bla<\/jats:italic><jats:sub>KPC<\/jats:sub>-containing <jats:italic>E. coli<\/jats:italic>. A <jats:italic>bla<\/jats:italic><jats:sub>KPC<\/jats:sub>-containing IncN and\/or IncFII<jats:sub>K<\/jats:sub> plasmid was identified in 77% (30\/39) of the <jats:italic>E. coli<\/jats:italic> and other bacterial species analyzed. Complete genome sequencing and comparative analysis of a <jats:italic>bla<\/jats:italic><jats:sub>KPC<\/jats:sub>-containing IncN plasmid from one of the <jats:italic>E. coli<\/jats:italic> strains demonstrated that this plasmid is present in the <jats:italic>K. pneumoniae<\/jats:italic> and <jats:italic>S. marcescens<\/jats:italic> strains from this patient, and is conserved among 13 of the <jats:italic>E. coli<\/jats:italic> and other bacterial species analyzed. Interestingly, while both IncFII<jats:sub>K<\/jats:sub> and IncN plasmids were prevalent among the strains analyzed, the IncN plasmids were more often identified in multiple bacterial species from the same patients, demonstrating a contribution of this IncN plasmid to the inter-genera dissemination of the <jats:italic>bla<\/jats:italic><jats:sub>KPC<\/jats:sub> genes between the <jats:italic>E. coli<\/jats:italic> and other bacterial species analyzed.<\/jats:p>","DOI":"10.1038\/s41598-018-28085-7","type":"journal-article","created":{"date-parts":[[2018,7,2]],"date-time":"2018-07-02T13:48:21Z","timestamp":1530539301000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Diversity among blaKPC-containing plasmids in Escherichia coli and other bacterial species isolated from the same patients"],"prefix":"10.1038","volume":"8","author":[{"given":"Tracy H.","family":"Hazen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Mettus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christi L.","family":"McElheny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah L.","family":"Bowler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sushma","family":"Nagaraj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohei","family":"Doi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7337-7154","authenticated-orcid":false,"given":"David A.","family":"Rasko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,7,6]]},"reference":[{"key":"28085_CR1","doi-asserted-by":"publisher","first-page":"1170","DOI":"10.3201\/eid2007.121004","volume":"20","author":"ME Falagas","year":"2014","unstructured":"Falagas, M. 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