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Orally administered C18 5-HT (0.1, 1 and 10\u2009mg\/kg) produced significant dose-dependent antinociceptive effects in formalin-, capsaicin- and glutamate-induced licking models. This compound also induced a significant increase in the response to thermal stimuli in the hot plate test, and its antinociceptive effect was not related to muscle relaxant or sedative actions. In a thermal hyperalgesia model, C18 5-HT presented an anti-hyperalgesic profile as evidenced by the increase in the response time of the animals. Furthermore, intraperitoneal (i.p) pretreatment with naloxone (a non-selective opioid receptor antagonist, 1\u2009mg\/kg), ondansetron (serotoninergic receptor antagonist (5-HT3 subtype), 0.5\u2009mg\/kg) or AM241 (CB1 cannabinoid receptor antagonist, 1\u2009mg\/kg) reversed the antinociceptive effects of C18 5-HT in the hot plate model. In the formalin-induced licking model, pretreatment with naloxone reversed the antinociceptive effects of C18 5-HT, as demonstrated by an increase in the paw licking response when compared with the C18 5-HT-treated group. These findings suggest that C18 5-HT has peripheral and central antinociceptive effects and that its mechanism of action involves, ate least in part, opioid, serotoninergic and cannabinoid pathways.<\/jats:p>","DOI":"10.1038\/s41598-018-28355-4","type":"journal-article","created":{"date-parts":[[2018,6,27]],"date-time":"2018-06-27T11:45:55Z","timestamp":1530099955000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["New \u03b2N-octadecanoyl-5-hydroxytryptamide: antinociceptive effect and possible mechanism of action in mice"],"prefix":"10.1038","volume":"8","author":[{"given":"Thais Biondino Sardella","family":"Giorno","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iris Gon\u00e7alvez da Silva","family":"Moreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia Moraes","family":"Rezende","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6870-6745","authenticated-orcid":false,"given":"Patricia Dias","family":"Fernandes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,7,3]]},"reference":[{"key":"28355_CR1","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1016\/j.jaad.2015.03.061","volume":"73","author":"NM Meeks","year":"2015","unstructured":"Meeks, N. 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