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This study explored EC permeability in malaria, and evaluated the potential use of FTY720 to restore EC permeability. ECs were incubated with sera from malaria patients (<jats:italic>P<\/jats:italic>. <jats:italic>vivax<\/jats:italic>, uncomplicated and complicated <jats:italic>P<\/jats:italic>. <jats:italic>falciparum<\/jats:italic> malaria). Cellular permeability was investigated using a fluorescein isothiocyanate (FITC)-dextran permeability assay. FTY720, an analogue of sphingosine-1-phosphate (S1P), was tested for its potential action in maintaining EC integrity. ECs incubated with sera from malaria patients with complicated <jats:italic>P<\/jats:italic>. <jats:italic>falciparum<\/jats:italic> showed higher fluorescein leakage compared with ECs incubated with sera from <jats:italic>P<\/jats:italic>. <jats:italic>vivax<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and uncomplicated <jats:italic>P<\/jats:italic>. <jats:italic>falciparum<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). ECs pretreated with FTY720 before incubation with malaria sera had significantly decreased fluorescein leakage compared with no FTY720 treatment. In addition, FTY720 treatment significantly reduced fluorescein leakage for both uncomplicated (at 45\u2009min) (<jats:italic>p<\/jats:italic>\u2009=\u20090.015), and complicated <jats:italic>P<\/jats:italic>. <jats:italic>falciparum<\/jats:italic> malaria (15\u2009min) (<jats:italic>p<\/jats:italic>\u2009=\u20090.043). The permeability increase induced by complicated <jats:italic>P<\/jats:italic>. <jats:italic>falciparum<\/jats:italic> sera was significantly reversed and prevented by FTY720 <jats:italic>in vitro<\/jats:italic>. FTY720 may have clinical applications to protect against endothelial barrier dysfunction in severe <jats:italic>P<\/jats:italic>. <jats:italic>falciparum<\/jats:italic> malaria.<\/jats:p>","DOI":"10.1038\/s41598-018-28536-1","type":"journal-article","created":{"date-parts":[[2018,7,13]],"date-time":"2018-07-13T15:11:54Z","timestamp":1531494714000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["FTY720 restores endothelial cell permeability induced by malaria sera"],"prefix":"10.1038","volume":"8","author":[{"given":"Karanyaporn","family":"Oggungwan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Supattra","family":"Glaharn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumate","family":"Ampawong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srivicha","family":"Krudsood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5157-1143","authenticated-orcid":false,"given":"Parnpen","family":"Viriyavejakul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,7,19]]},"reference":[{"key":"28536_CR1","unstructured":"WHO. 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