{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:11:26Z","timestamp":1792674686957,"version":"4.5.0"},"reference-count":55,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2018,7,17]],"date-time":"2018-07-17T00:00:00Z","timestamp":1531785600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2018,7,17]],"date-time":"2018-07-17T00:00:00Z","timestamp":1531785600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Isolating and maintaining the appropriate stem cell for large scale cell culture is essential in tissue engineering or food production. For bovine satellite cells an optimized isolation and purification protocol is lacking and there is also no detailed understanding on the factors that maintain stemness of these cells. Here, we set up a fluorescence-activated cell sorting strategy to enrich bovine satellite cells. We found that p38-MAPK signalling is activated and PAX7 expression is gradually lost during satellite cell proliferation. The p38 inhibitor (SB203580) treatment maintained PAX7 expression but inhibited the fusion of satellite cells in a concentration-dependent way in short-term incubation. The mechanism of p38 inhibition was confirmed by inhibiting canonical p38 signalling, i.e. HSP27. Long-term culture with an appropriate concentration of p38i enhanced the proliferation and PAX7 expression, while the differentiation capacity recovered and was enhanced compared to vehicle control. These studies indicate that bovine satellite cells maintenance depends on cell purity and p38 MAPK signalling. Inhibition of p38 MAPK signaling is a promising strategy to facilitate large scale cell expansion of primary cells for tissue engineering and cultured meat purposes.<\/jats:p>","DOI":"10.1038\/s41598-018-28746-7","type":"journal-article","created":{"date-parts":[[2018,7,11]],"date-time":"2018-07-11T11:33:25Z","timestamp":1531308805000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":168,"title":["Maintaining bovine satellite cells stemness through p38 pathway"],"prefix":"10.1038","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5807-0103","authenticated-orcid":false,"given":"Shijie","family":"Ding","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. N. M","family":"Swennen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Messmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mick","family":"Gagliardi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dani\u00ebl G. M.","family":"Molin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0708-891X","authenticated-orcid":false,"given":"Chunbao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark J.","family":"Post","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,7,17]]},"reference":[{"key":"28746_CR1","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1083\/jcb.9.2.493","volume":"9","author":"A Mauro","year":"1961","unstructured":"Mauro, A. Satellite cell of skeletal muscle fibers. J Biophys Biochem Cytol 9, 493\u2013495 (1961).","journal-title":"J Biophys Biochem Cytol"},{"key":"28746_CR2","doi-asserted-by":"publisher","first-page":"954","DOI":"10.1038\/nature03572","volume":"435","author":"J Gros","year":"2005","unstructured":"Gros, J., Manceau, M., Thome, V. & Marcelle, C. A common somitic origin for embryonic muscle progenitors and satellite cells. Nature 435, 954\u2013958, https:\/\/doi.org\/10.1038\/nature03572 (2005).","journal-title":"Nature"},{"key":"28746_CR3","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/j.ydbio.2007.06.006","volume":"308","author":"S Biressi","year":"2007","unstructured":"Biressi, S., Molinaro, M. & Cossu, G. Cellular heterogeneity during vertebrate skeletal muscle development. Dev Biol 308, 281\u2013293, https:\/\/doi.org\/10.1016\/j.ydbio.2007.06.006 (2007).","journal-title":"Dev Biol"},{"key":"28746_CR4","doi-asserted-by":"publisher","first-page":"948","DOI":"10.1038\/nature03594","volume":"435","author":"F Relaix","year":"2005","unstructured":"Relaix, F., Rocancourt, D., Mansouri, A. & Buckingham, M. A Pax3\/Pax7-dependent population of skeletal muscle progenitor cells. Nature 435, 948\u2013953, https:\/\/doi.org\/10.1038\/nature03594 (2005).","journal-title":"Nature"},{"key":"28746_CR5","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1038\/nrm.2016.7","volume":"17","author":"AE Almada","year":"2016","unstructured":"Almada, A. E. & Wagers, A. J. Molecular circuitry of stem cell fate in skeletal muscle regeneration, ageing and disease. Nature reviews. Molecular cell biology 17, 267\u2013279, https:\/\/doi.org\/10.1038\/nrm.2016.7 (2016).","journal-title":"Nature reviews. Molecular cell biology"},{"key":"28746_CR6","doi-asserted-by":"publisher","first-page":"1663","DOI":"10.1007\/s00018-014-1819-5","volume":"72","author":"X Fu","year":"2015","unstructured":"Fu, X., Wang, H. T. & Hu, P. Stem cell activation in skeletal muscle regeneration. Cell Mol Life Sci 72, 1663\u20131677, https:\/\/doi.org\/10.1007\/s00018-014-1819-5 (2015).","journal-title":"Cell Mol Life Sci"},{"key":"28746_CR7","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1016\/j.meatsci.2012.04.008","volume":"92","author":"MJ Post","year":"2012","unstructured":"Post, M. J. Cultured meat from stem cells: challenges and prospects. Meat science 92, 297\u2013301, https:\/\/doi.org\/10.1016\/j.meatsci.2012.04.008 (2012).","journal-title":"Meat science"},{"key":"28746_CR8","doi-asserted-by":"publisher","first-page":"6117","DOI":"10.1021\/es200130u","volume":"45","author":"HL Tuomisto","year":"2011","unstructured":"Tuomisto, H. L. & de Mattos, M. J. T. Environmental Impacts of Cultured Meat Production. Environ Sci Technol 45, 6117\u20136123, https:\/\/doi.org\/10.1021\/Es200130u (2011).","journal-title":"Environ Sci Technol"},{"key":"28746_CR9","doi-asserted-by":"publisher","first-page":"1039","DOI":"10.1002\/jsfa.6474","volume":"94","author":"MJ Post","year":"2014","unstructured":"Post, M. J. Cultured beef: medical technology to produce food. J Sci Food Agric 94, 1039\u20131041, https:\/\/doi.org\/10.1002\/jsfa.6474 (2014).","journal-title":"J Sci Food Agric"},{"key":"28746_CR10","doi-asserted-by":"publisher","first-page":"1751","DOI":"10.2527\/jas.2010-3568","volume":"89","author":"J Li","year":"2011","unstructured":"Li, J. et al. Evidence of heterogeneity within bovine satellite cells isolated from young and adult animals. J Anim Sci 89, 1751\u20131757 (2011).","journal-title":"J Anim Sci"},{"key":"28746_CR11","doi-asserted-by":"publisher","first-page":"885","DOI":"10.1007\/s11626-015-9916-9","volume":"51","author":"K Will","year":"2015","unstructured":"Will, K., Schering, L., Albrecht, E., Kalbe, C. & Maak, S. Differentiation of bovine satellite cell-derived myoblasts under different culture conditions. In vitro cellular & developmental biology. Animal 51, 885\u2013889, https:\/\/doi.org\/10.1007\/s11626-015-9916-9 (2015).","journal-title":"In vitro cellular & developmental biology. Animal"},{"key":"28746_CR12","doi-asserted-by":"crossref","unstructured":"Coles, C. A. et al. Proliferation Rates of Bovine Primary Muscle Cells Relate to Liveweight and Carcase Weight in Cattle. Plos One 10 (2015).","DOI":"10.1371\/journal.pone.0124468"},{"key":"28746_CR13","doi-asserted-by":"publisher","first-page":"752","DOI":"10.1038\/nbt.3576","volume":"34","author":"M Quarta","year":"2016","unstructured":"Quarta, M. et al. An artificial niche preserves the quiescence of muscle stem cells and enhances their therapeutic efficacy. Nat Biotechnol 34, 752\u2013759, https:\/\/doi.org\/10.1038\/nbt.3576 (2016).","journal-title":"Nat Biotechnol"},{"key":"28746_CR14","doi-asserted-by":"publisher","first-page":"889","DOI":"10.1038\/nm.4116","volume":"22","author":"M Rozo","year":"2016","unstructured":"Rozo, M., Li, L. & Fan, C. M. Targeting beta1-integrin signaling enhances regeneration in aged and dystrophic muscle in mice. Nat Med 22, 889\u2013896, https:\/\/doi.org\/10.1038\/nm.4116 (2016).","journal-title":"Nat Med"},{"key":"28746_CR15","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1016\/j.stemcr.2015.07.016","volume":"5","author":"X Xu","year":"2015","unstructured":"Xu, X. et al. Human Satellite Cell Transplantation and Regeneration from Diverse Skeletal Muscles. Stem Cell Rep 5, 419\u2013434, https:\/\/doi.org\/10.1016\/j.stemcr.2015.07.016 (2015).","journal-title":"Stem Cell Rep"},{"key":"28746_CR16","doi-asserted-by":"publisher","first-page":"502","DOI":"10.1038\/nature07384","volume":"456","author":"A Sacco","year":"2008","unstructured":"Sacco, A., Doyonnas, R., Kraft, P., Vitorovic, S. & Blau, H. M. Self-renewal and expansion of single transplanted muscle stem cells. Nature 456, 502\u2013506, https:\/\/doi.org\/10.1038\/nature07384 (2008).","journal-title":"Nature"},{"key":"28746_CR17","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1016\/j.stemcr.2015.08.004","volume":"5","author":"GW Charville","year":"2015","unstructured":"Charville, G. W. et al. Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells. Stem Cell Rep 5, 621\u2013632, https:\/\/doi.org\/10.1016\/j.stemcr.2015.08.004 (2015).","journal-title":"Stem Cell Rep"},{"key":"28746_CR18","doi-asserted-by":"publisher","first-page":"17003","DOI":"10.1038\/cddiscovery.2017.3","volume":"3","author":"S Ding","year":"2017","unstructured":"Ding, S. et al. Characterization and isolation of highly purified porcine satellite cells. Cell Death Discov 3, 17003, https:\/\/doi.org\/10.1038\/cddiscovery.2017.3 (2017).","journal-title":"Cell Death Discov"},{"key":"28746_CR19","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1038\/cr.2015.58","volume":"25","author":"X Fu","year":"2015","unstructured":"Fu, X. et al. Combination of inflammation-related cytokines promotes long-term muscle stem cell expansion. Cell Res 25, 655\u2013673, https:\/\/doi.org\/10.1038\/cr.2015.58 (2015).","journal-title":"Cell Res"},{"key":"28746_CR20","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1002\/stem.317","volume":"28","author":"DF Pisani","year":"2010","unstructured":"Pisani, D. F. et al. Isolation of a Highly Myogenic CD34-Negative Subset of Human Skeletal Muscle Cells Free of Adipogenic Potential. Stem Cells 28, 753\u2013764, https:\/\/doi.org\/10.1002\/stem.317 (2010).","journal-title":"Stem Cells"},{"key":"28746_CR21","doi-asserted-by":"publisher","first-page":"3997","DOI":"10.1158\/0008-5472.CAN-15-0173","volume":"75","author":"A Igea","year":"2015","unstructured":"Igea, A. & Nebreda, A. R. The Stress Kinase p38 alpha as a Target for Cancer Therapy. Cancer Res 75, 3997\u20134002 (2015).","journal-title":"Cancer Res"},{"key":"28746_CR22","doi-asserted-by":"publisher","first-page":"91","DOI":"10.3389\/fcell.2016.00091","volume":"4","author":"J Segales","year":"2016","unstructured":"Segales, J., Perdiguero, E. & Munoz-Canoves, P. Regulation of Muscle Stem Cell Functions: A Focus on the p38 MAPK Signaling Pathway. Front Cell Dev Biol 4, 91, https:\/\/doi.org\/10.3389\/fcell.2016.00091 (2016).","journal-title":"Front Cell Dev Biol"},{"key":"28746_CR23","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1016\/j.stem.2012.05.025","volume":"11","author":"A Troy","year":"2012","unstructured":"Troy, A. et al. Coordination of satellite cell activation and self-renewal by Par-complex-dependent asymmetric activation of p38alpha\/beta MAPK. Cell stem cell 11, 541\u2013553, https:\/\/doi.org\/10.1016\/j.stem.2012.05.025 (2012).","journal-title":"Cell stem cell"},{"key":"28746_CR24","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1038\/nm.3464","volume":"20","author":"BD Cosgrove","year":"2014","unstructured":"Cosgrove, B. D. et al. Rejuvenation of the muscle stem cell population restores strength to injured aged muscles. Nat Med 20, 255\u2013264, https:\/\/doi.org\/10.1038\/nm.3464 (2014).","journal-title":"Nat Med"},{"key":"28746_CR25","doi-asserted-by":"publisher","first-page":"1597","DOI":"10.1002\/stem.1399","volume":"31","author":"P Brien","year":"2013","unstructured":"Brien, P., Pugazhendhi, D., Woodhouse, S., Oxley, D. & Pell, J. M. p38alpha MAPK regulates adult muscle stem cell fate by restricting progenitor proliferation during postnatal growth and repair. Stem Cells 31, 1597\u20131610, https:\/\/doi.org\/10.1002\/stem.1399 (2013).","journal-title":"Stem Cells"},{"key":"28746_CR26","doi-asserted-by":"publisher","first-page":"777","DOI":"10.1016\/S0092-8674(00)00066-0","volume":"102","author":"P Seale","year":"2000","unstructured":"Seale, P. et al. Pax7 is required for the specification of myogenic satellite cells. Cell 102, 777\u2013786, https:\/\/doi.org\/10.1016\/S0092-8674(00)00066-0 (2000).","journal-title":"Cell"},{"key":"28746_CR27","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1083\/jcb.200508044","volume":"172","author":"F Relaix","year":"2006","unstructured":"Relaix, F. et al. Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells. J Cell Biol 172, 91\u2013102, https:\/\/doi.org\/10.1083\/jcb.200508044 (2006).","journal-title":"J Cell Biol"},{"key":"28746_CR28","doi-asserted-by":"publisher","first-page":"3194","DOI":"10.1634\/stemcells.2007-1017","volume":"26","author":"D Bosnakovski","year":"2008","unstructured":"Bosnakovski, D. et al. Prospective Isolation of Skeletal Muscle Stem Cells with a Pax7 Reporter. Stem Cells 26, 3194\u20133204, https:\/\/doi.org\/10.1634\/stemcells.2007-1017 (2008).","journal-title":"Stem Cells"},{"key":"28746_CR29","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1016\/j.cell.2005.05.010","volume":"122","author":"CA Collins","year":"2005","unstructured":"Collins, C. A. et al. Stem cell function, self-renewal, and behavioral heterogeneity of cells from the adult muscle satellite cell niche. Cell 122, 289\u2013301, https:\/\/doi.org\/10.1016\/j.cell.2005.05.010 (2005).","journal-title":"Cell"},{"key":"28746_CR30","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1177\/002215540405200205","volume":"52","author":"S Sajko","year":"2004","unstructured":"Sajko, S. et al. Frequency of M-cadherin-stained satellite cells declines in human muscles during aging. J Histochem Cytochem 52, 179\u2013185, https:\/\/doi.org\/10.1177\/002215540405200205 (2004).","journal-title":"J Histochem Cytochem"},{"key":"28746_CR31","doi-asserted-by":"publisher","unstructured":"Badenes, S. M., Fernandes, T. G., Rodrigues, C. A., Diogo, M. M. & Cabral, J. M. Microcarrier-based platforms for in vitro expansion and differentiation of human pluripotent stem cells in bioreactor culture systems. J Biotechnol, https:\/\/doi.org\/10.1016\/j.jbiotec.2016.07.023 (2016).","DOI":"10.1016\/j.jbiotec.2016.07.023"},{"key":"28746_CR32","doi-asserted-by":"publisher","first-page":"1048","DOI":"10.1002\/biot.201500532","volume":"11","author":"A Mizukami","year":"2016","unstructured":"Mizukami, A. et al. Stirred tank bioreactor culture combined with serum-\/xenogeneic-free culture medium enables an efficient expansion of umbilical cord-derived mesenchymal stem\/stromal cells. Biotechnol J 11, 1048\u20131059, https:\/\/doi.org\/10.1002\/biot.201500532 (2016).","journal-title":"Biotechnol J"},{"key":"28746_CR33","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1016\/S2095-3119(14)60889-3","volume":"14","author":"MSM Moritz","year":"2015","unstructured":"Moritz, M. S. M., Verbruggen, S. E. L. & Post, M. J. Alternatives for large-scale production of cultured beef: A review. J Integr Agr 14, 208\u2013216, https:\/\/doi.org\/10.1016\/S2095-3119(14)60889-3 (2015).","journal-title":"J Integr Agr"},{"key":"28746_CR34","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1007\/978-1-61779-343-1_3","volume":"798","author":"A Pasut","year":"2012","unstructured":"Pasut, A., Oleynik, P. & Rudnicki, M. A. Isolation of muscle stem cells by fluorescence activated cell sorting cytometry. Methods in molecular biology 798, 53\u201364, https:\/\/doi.org\/10.1007\/978-1-61779-343-1_3 (2012).","journal-title":"Methods in molecular biology"},{"key":"28746_CR35","doi-asserted-by":"publisher","first-page":"1612","DOI":"10.1038\/nprot.2015.110","volume":"10","author":"L Liu","year":"2015","unstructured":"Liu, L., Cheung, T. H., Charville, G. W. & Rando, T. A. Isolation of skeletal muscle stem cells by fluorescence-activated cell sorting. Nat Protoc 10, 1612\u20131624, https:\/\/doi.org\/10.1038\/nprot.2015.110 (2015).","journal-title":"Nat Protoc"},{"key":"28746_CR36","doi-asserted-by":"publisher","first-page":"2064","DOI":"10.1126\/science.1114758","volume":"309","author":"D Montarras","year":"2005","unstructured":"Montarras, D. et al. Direct isolation of satellite cells for skeletal muscle regeneration. Science 309, 2064\u20132067, https:\/\/doi.org\/10.1126\/science.1114758 (2005).","journal-title":"Science"},{"key":"28746_CR37","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1083\/jcb.200408066","volume":"169","author":"NC Jones","year":"2005","unstructured":"Jones, N. C. et al. Thep38 alpha\/beta MAPK functions as a molecular switch to activate the quiescent satellite cell. J Cell Biol 169, 105\u2013116 (2005).","journal-title":"J Cell Biol"},{"key":"28746_CR38","doi-asserted-by":"publisher","first-page":"738","DOI":"10.1038\/ng1378","volume":"36","author":"C Simone","year":"2004","unstructured":"Simone, C. et al. p38 pathway targets SWI-SNF chromatin-remodeling complex to muscle-specific loci. Nat Genet 36, 738\u2013743, https:\/\/doi.org\/10.1038\/ng1378 (2004).","journal-title":"Nat Genet"},{"key":"28746_CR39","doi-asserted-by":"publisher","first-page":"961","DOI":"10.1016\/j.celrep.2014.04.016","volume":"7","author":"S Yennek","year":"2014","unstructured":"Yennek, S., Burute, M., Thery, M. & Tajbakhsh, S. Cell adhesion geometry regulates non-random DNA segregation and asymmetric cell fates in mouse skeletal muscle stem cells. Cell Rep 7, 961\u2013970, https:\/\/doi.org\/10.1016\/j.celrep.2014.04.016 (2014).","journal-title":"Cell Rep"},{"key":"28746_CR40","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1038\/nm.3465","volume":"20","author":"JD Bernet","year":"2014","unstructured":"Bernet, J. D. et al. p38 MAPK signaling underlies a cell-autonomous loss of stem cell self-renewal in skeletal muscle of aged mice. Nat Med 20, 265\u2013271, https:\/\/doi.org\/10.1038\/nm.3465 (2014).","journal-title":"Nat Med"},{"key":"28746_CR41","doi-asserted-by":"publisher","first-page":"386","DOI":"10.1093\/jmcb\/mjs045","volume":"4","author":"QC Liu","year":"2012","unstructured":"Liu, Q. C. et al. Comparative expression profiling identifies differential roles for Myogenin and p38alpha MAPK signaling in myogenesis. J Mol Cell Biol 4, 386\u2013397, https:\/\/doi.org\/10.1093\/jmcb\/mjs045 (2012).","journal-title":"J Mol Cell Biol"},{"key":"28746_CR42","doi-asserted-by":"publisher","first-page":"191","DOI":"10.4161\/cc.10.2.14441","volume":"10","author":"C Mozzetta","year":"2011","unstructured":"Mozzetta, C. et al. Selective control of Pax7 expression by TNF-activated p38alpha\/polycomb repressive complex 2 (PRC2) signaling during muscle satellite cell differentiation. Cell Cycle 10, 191\u2013198, https:\/\/doi.org\/10.4161\/cc.10.2.14570 (2011).","journal-title":"Cell Cycle"},{"key":"28746_CR43","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1016\/j.stem.2010.08.013","volume":"7","author":"D Palacios","year":"2010","unstructured":"Palacios, D. et al. TNF\/p38alpha\/polycomb signaling to Pax7 locus in satellite cells links inflammation to the epigenetic control of muscle regeneration. Cell stem cell 7, 455\u2013469, https:\/\/doi.org\/10.1016\/j.stem.2010.08.013 (2010).","journal-title":"Cell stem cell"},{"key":"28746_CR44","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1016\/j.cell.2011.11.049","volume":"148","author":"P Rocheteau","year":"2012","unstructured":"Rocheteau, P., Gayraud-Morel, B., Siegl-Cachedenier, I., Blasco, M. A. & Tajbakhsh, S. A Subpopulation of Adult Skeletal Muscle Stem Cells Retains All Template DNA Strands after Cell Division. Cell 148, 112\u2013125 (2012).","journal-title":"Cell"},{"key":"28746_CR45","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1016\/j.ydbio.2004.08.015","volume":"275","author":"HC Olguin","year":"2004","unstructured":"Olguin, H. C. & Olwin, B. B. Pax-7 up-regulation inhibits myogenesis and cell cycle progression in satellite cells: a potential mechanism for self-renewal. Dev Biol 275, 375\u2013388, https:\/\/doi.org\/10.1016\/j.ydbio.2004.08.015 (2004).","journal-title":"Dev Biol"},{"key":"28746_CR46","doi-asserted-by":"publisher","first-page":"16474","DOI":"10.1073\/pnas.1307680110","volume":"110","author":"J von Maltzahn","year":"2013","unstructured":"von Maltzahn, J., Jones, A. E., Parks, R. J. & Rudnicki, M. A. Pax7 is critical for the normal function of satellite cells in adult skeletal muscle. Proceedings of the National Academy of Sciences of the United States of America 110, 16474\u201316479, https:\/\/doi.org\/10.1073\/pnas.1307680110 (2013).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"28746_CR47","doi-asserted-by":"publisher","first-page":"1078","DOI":"10.1126\/science.1191035","volume":"329","author":"PM Gilbert","year":"2010","unstructured":"Gilbert, P. M. et al. Substrate elasticity regulates skeletal muscle stem cell self-renewal in culture. Science 329, 1078\u20131081, https:\/\/doi.org\/10.1126\/science.1191035 (2010).","journal-title":"Science"},{"key":"28746_CR48","doi-asserted-by":"publisher","unstructured":"Urciuolo, A. et al. Collagen VI regulates satellite cell self-renewal and muscle regeneration. Nat Commun 4, https:\/\/doi.org\/10.1038\/Ncomms2964 (2013).","DOI":"10.1038\/Ncomms2964"},{"key":"28746_CR49","doi-asserted-by":"publisher","first-page":"C1338","DOI":"10.1152\/ajpcell.00015.2009","volume":"296","author":"KJ Boonen","year":"2009","unstructured":"Boonen, K. J., Rosaria-Chak, K. Y., Baaijens, F. P., van der Schaft, D. W. & Post, M. J. Essential environmental cues from the satellite cell niche: optimizing proliferation and differentiation. American journal of physiology. Cell physiology 296, C1338\u20131345, https:\/\/doi.org\/10.1152\/ajpcell.00015.2009 (2009).","journal-title":"American journal of physiology. Cell physiology"},{"key":"28746_CR50","doi-asserted-by":"publisher","first-page":"3929","DOI":"10.1096\/fj.201600757R","volume":"30","author":"T Chaillou","year":"2016","unstructured":"Chaillou, T. & Lanner, J. T. Regulation of myogenesis and skeletal muscle regeneration: effects of oxygen levels on satellite cell activity. Faseb J 30, 3929\u20133941, https:\/\/doi.org\/10.1096\/fj.201600757R (2016).","journal-title":"Faseb J"},{"key":"28746_CR51","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1016\/j.cellbi.2008.03.017","volume":"32","author":"SH Kook","year":"2008","unstructured":"Kook, S. H. et al. Hypoxia affects positively the proliferation of bovine satellite cells and their myogenic differentiation through up-regulation of MyoD. Cell biology international 32, 871\u2013878, https:\/\/doi.org\/10.1016\/j.cellbi.2008.03.017 (2008).","journal-title":"Cell biology international"},{"key":"28746_CR52","doi-asserted-by":"publisher","first-page":"2857","DOI":"10.1242\/dev.079665","volume":"139","author":"W Liu","year":"2012","unstructured":"Liu, W. et al. Hypoxia promotes satellite cell self-renewal and enhances the efficiency of myoblast transplantation. Development 139, 2857\u20132865, https:\/\/doi.org\/10.1242\/dev.079665 (2012).","journal-title":"Development"},{"key":"28746_CR53","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1016\/j.celrep.2016.06.001","volume":"16","author":"A Pasut","year":"2016","unstructured":"Pasut, A. et al. Notch Signaling Rescues Loss of Satellite Cells Lacking Pax7 and Promotes Brown Adipogenic Differentiation. Cell Rep 16, 333\u2013343, https:\/\/doi.org\/10.1016\/j.celrep.2016.06.001 (2016).","journal-title":"Cell Rep"},{"key":"28746_CR54","doi-asserted-by":"publisher","first-page":"961","DOI":"10.1038\/nature07182","volume":"454","author":"P Seale","year":"2008","unstructured":"Seale, P. et al. PRDM16 controls a brown fat\/skeletal muscle switch. Nature 454, 961\u2013967, https:\/\/doi.org\/10.1038\/nature07182 (2008).","journal-title":"Nature"},{"key":"28746_CR55","doi-asserted-by":"publisher","first-page":"5393","DOI":"10.1242\/jcs.01419","volume":"117","author":"G Shefer","year":"2004","unstructured":"Shefer, G., Wleklinski-Lee, M. & Yablonka-Reuveni, Z. Skeletal muscle satellite cells can spontaneously enter an alternative mesenchymal pathway. J Cell Sci 117, 5393\u20135404, https:\/\/doi.org\/10.1242\/jcs.01419 (2004).","journal-title":"J Cell Sci"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-018-28746-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-018-28746-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-018-28746-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T07:25:32Z","timestamp":1671607532000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-018-28746-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,17]]},"references-count":55,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2018,12]]}},"alternative-id":["28746"],"URL":"https:\/\/doi.org\/10.1038\/s41598-018-28746-7","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,17]]},"assertion":[{"value":"13 December 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 June 2018","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 July 2018","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Mark J Post is the co-founder of MosaMeat, a company that aims to commercialize cultured meat. The other authors declare no conflict of interest.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"10808"}}