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The defensive potential of glucosinolates, a group of amino acid-derived thioglucosides present in plants of the Brassicales order, arises mainly from their rapid breakdown upon tissue disruption resulting in formation of toxic isothiocyanates. Larvae of\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>rapae<\/jats:italic>\n                    are able to feed exclusively on glucosinolate-containing plants due to expression of a nitrile-specifier protein in their gut which redirects glucosinolate breakdown to the formation of nitriles. The release of equimolar amounts of cyanide upon further metabolism of the benzylglucosinolate-derived nitrile suggests that the larvae are also equipped with efficient means of cyanide detoxification such as \u03b2-cyanoalanine synthases or rhodaneses. While insect \u03b2-cyanoalanine synthases have recently been identified at the molecular level, no sequence information was available of characterized insect rhodaneses. Here, we identify and characterize two single-domain rhodaneses from\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>rapae<\/jats:italic>\n                    , PrTST1 and PrTST2. The enzymes differ in their kinetic properties, predicted subcellular localization and expression in\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>rapae<\/jats:italic>\n                    indicating different physiological roles. Phylogenetic analysis together with putative lepidopteran rhodanese sequences indicates an expansion of the rhodanese family in Pieridae.\n                  <\/jats:p>","DOI":"10.1038\/s41598-018-29148-5","type":"journal-article","created":{"date-parts":[[2018,7,11]],"date-time":"2018-07-11T09:11:51Z","timestamp":1531300311000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Molecular identification and characterization of rhodaneses from the insect herbivore Pieris rapae"],"prefix":"10.1038","volume":"8","author":[{"given":"Anna-Maria","family":"Steiner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Busching","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9821-7731","authenticated-orcid":false,"given":"Heiko","family":"Vogel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0914-8453","authenticated-orcid":false,"given":"Ute","family":"Wittstock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,7,17]]},"reference":[{"key":"29148_CR1","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1111\/j.1558-5646.1964.tb01674.x","volume":"18","author":"PR Ehrlich","year":"1964","unstructured":"Ehrlich, P. 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