{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T23:04:48Z","timestamp":1789167888611,"version":"build-2803163510"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2018,7,25]],"date-time":"2018-07-25T00:00:00Z","timestamp":1532476800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2018,7,25]],"date-time":"2018-07-25T00:00:00Z","timestamp":1532476800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The use of insecticides has been a primary tool to manage <jats:italic>Bactrocera dorsalis<\/jats:italic> in Pakistan; however, recent reports of field control failures necessitate mapping out the insecticide resistance problem. Therefore, eight field strains from Pakistan, were evaluated for their resistance against trichlorfon and spinosad. Compared with a reference strain, six field strains showed high levels of resistance to trichlorfon, while two field strains expressed intermediate resistance. In case of spinosad, five field strains fell in the susceptible range, whereas, the rest of the strains represented minor resistance. Correlation analysis between LD<jats:sub>50<\/jats:sub> values of trichlorfon and spinosad of all the field strains revealed non-significant association, suggesting the possibility of lack of cross-resistance between both insecticides. Synergism bioassays implementing <jats:italic>S<\/jats:italic>,<jats:italic>S<\/jats:italic>,<jats:italic>S<\/jats:italic>-tributylphosphorotrithioate (DEF) and piperonyl butoxide (PBO) revealed that the LD<jats:sub>50<\/jats:sub> values of trichlorfon in the presence of either DEF or PBO in seven field strains were significantly reduced. However, DEF and PBO had a non-significant effect on synergizing spinosad toxicity. The results revealed resistance to trichlorfon in field strains of <jats:italic>B<\/jats:italic>. <jats:italic>dorsalis<\/jats:italic>, which might be metabolic-based. Absence or minor resistance to spinosad and lack of cross-resistance to trichlorfon, suggest that spinosad could be a potential candidate for managing <jats:italic>B<\/jats:italic>. <jats:italic>dorsalis<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/s41598-018-29622-0","type":"journal-article","created":{"date-parts":[[2018,7,19]],"date-time":"2018-07-19T11:02:23Z","timestamp":1531998143000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Trichlorfon and spinosad resistance survey and preliminary determination of the resistance mechanism in Pakistani field strains of Bactrocera dorsalis"],"prefix":"10.1038","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7209-4750","authenticated-orcid":false,"given":"Hafiz Azhar Ali","family":"Khan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waseem","family":"Akram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,7,25]]},"reference":[{"key":"29622_CR1","doi-asserted-by":"publisher","first-page":"1682","DOI":"10.1603\/0022-0493-97.5.1682","volume":"97","author":"J-C Hsu","year":"2004","unstructured":"Hsu, J.-C., Feng, H.-T. & Wu, W.-J. 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