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In order to obtain an attenuated<jats:italic>Leishmania<\/jats:italic>parasite for vaccine purposes, we generated<jats:italic>L. infantum KHARON1<\/jats:italic>(<jats:italic>KH1<\/jats:italic>) null mutants (\u0394<jats:italic>Likh1<\/jats:italic>). This gene was previously associated with growth defects in<jats:italic>L. mexicana<\/jats:italic>. \u0394<jats:italic>Likh1<\/jats:italic>was obtained and confirmed by PCR, qPCR and<jats:italic>Southern blot<\/jats:italic>. We also generate a<jats:italic>KH1<\/jats:italic>complemented line with the introduction of episomal copies of<jats:italic>KH1<\/jats:italic>. Although \u0394<jats:italic>Likh1<\/jats:italic>promastigote forms exhibited a growth pattern similar to the wild-type line, they differ in morphology without affecting parasite viability.<jats:italic>L. infantum<\/jats:italic>KH1-deficient amastigotes were unable to sustain experimental infection in macrophages, forming multinucleate cells which was confirmed by<jats:italic>in vivo<\/jats:italic>attenuation phenotype. The cell cycle analysis of \u0394<jats:italic>Likh1<\/jats:italic>amastigotes showed arrested cells at G<jats:sub>2<\/jats:sub>\/M phase. \u0394<jats:italic>Likh1-<\/jats:italic>immunized mice presented reduced parasite burden upon challenging with virulent<jats:italic>L. infantum<\/jats:italic>, when compared to na\u00efve mice. An effect associated with increased<jats:italic>Li<\/jats:italic>SLA-specific IgG serum levels and IL-17 production. Thus, \u0394<jats:italic>Likh1<\/jats:italic>parasites present an infective-attenuated phenotype due to a cytokinesis defect, whereas it induces immunity against visceral leishmaniasis in mouse model, being a candidate for antileishmanial vaccine purposes.<\/jats:p>","DOI":"10.1038\/s41598-018-30076-7","type":"journal-article","created":{"date-parts":[[2018,7,27]],"date-time":"2018-07-27T11:24:57Z","timestamp":1532690697000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Growth arrested live-attenuated Leishmania infantum KHARON1 null mutants display cytokinesis defect and protective immunity in mice"],"prefix":"10.1038","volume":"8","author":[{"given":"Ana Maria Murta","family":"Santi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliane Sousa","family":"Lanza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiza Guimar\u00e3es","family":"Tunes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline Ara\u00fajo","family":"Fiuza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ga\u00e9tan","family":"Roy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra da Silva","family":"Orfan\u00f3","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9a Teixeira","family":"de Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Fr\u00e9zard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7641-6585","authenticated-orcid":false,"given":"Andr\u00e9 Lu\u00eds Branco de","family":"Barros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvane Maria Fonseca","family":"Murta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4688-2462","authenticated-orcid":false,"given":"Rubens Lima","family":"do Monte-Neto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,8,2]]},"reference":[{"key":"30076_CR1","doi-asserted-by":"publisher","first-page":"e35671","DOI":"10.1371\/journal.pone.0035671","volume":"7","author":"J Alvar","year":"2012","unstructured":"Alvar, J. et al. 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