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Improvement the therapeutic efficacy of Bas an anti-leishmania agent through increasing the effective dose was achieved by using a novel solvent and phase separation method for K synthesis. The synthesized K with the size of 102\u2009nm and Betulinic acid-nanochitosan (BK) with the size of 124\u2009nm and drug loading efficiency of 93%, cellular uptake of 97.5% with the slow drug release pattern was prepared. To increase the therapeutic dose, a modified 10% acetic acid solvent was used. The<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>results showed that the nanodrug of BK was non toxic by 100% and BK20\u2009mg\/kg could completely performed the wound healing and inhibit the parasite in a large extent (P\u2009\u02c2\u20090.001) compared to other groups. 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