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Second, we aimed to assess biofilm production dynamics after extubation. We prospectively analyzed 70 ETT samples obtained from pigs randomized to be untreated (controls, n\u2009=\u200920), or treated with vancomycin (n\u2009=\u200932) or linezolid (n\u2009=\u200918). A clinical MRSA strain (MRSA-in) was inoculated in pigs to create a pneumonia model, before treating with antibiotics. Tracheally intubated pigs with MRSA severe pneumonia, were mechanically ventilated for 69\u2009\u00b1\u200916\u2009hours. All MRSA isolates retrieved from ETTs (ETT-MRSA) were tested for their <jats:italic>in vitro<\/jats:italic> biofilm production by microtiter plate assay. <jats:italic>In vitro<\/jats:italic> biofilm production of MRSA isolates was sequentially studied over the next 8 days post-extubation to assess biofilm capability dynamics over time. All experiments were performed under ambient air (O<jats:sub>2<\/jats:sub>) or ambient air supplemented with 5% CO<jats:sub>2<\/jats:sub>. We collected 52 ETT-MRSA isolates (placebo N\u2009=\u200919, linezolid N\u2009=\u200911, and vancomycin N\u2009=\u200922) that were clonally identical to the MRSA-in. Among the ETT-MRSA isolates, biofilm production more than doubled after extubation in 40% and 50% under 5% CO<jats:sub>2<\/jats:sub> and O<jats:sub>2<\/jats:sub>, respectively. Systemic antibiotic treatment during intubation did not affect this outcome. Under both atmospheric conditions, biofilm production for MRSA-in was at least doubled for 9 ETT-MRSA isolates, and assessment of these showed that biofilm production decreased progressively over a 4-day period after extubation. In conclusion, a weak biofilm producer MRSA strain significantly enhances its biofilm production within an ETT, but it is influenced by the ETT environment rather than by the systemic treatment used during intubation or by the atmospheric conditions used for bacterial growth.<\/jats:p>","DOI":"10.1038\/s41598-018-30494-7","type":"journal-article","created":{"date-parts":[[2018,8,3]],"date-time":"2018-08-03T09:36:41Z","timestamp":1533289001000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Assessment of in vivo versus in vitro biofilm formation of clinical methicillin-resistant Staphylococcus aureus isolates from endotracheal tubes"],"prefix":"10.1038","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7528-9636","authenticated-orcid":false,"given":"Laia","family":"Fern\u00e1ndez-Barat","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5572-5883","authenticated-orcid":false,"given":"Soumaya","family":"Ben-Aicha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Motos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordi","family":"Vila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesc","family":"Marco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Montserrat","family":"Rigol","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Mu\u00f1oz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianluigi","family":"Li Bassi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miquel","family":"Ferrer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antoni","family":"Torres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,8,9]]},"reference":[{"key":"30494_CR1","doi-asserted-by":"publisher","first-page":"1533","DOI":"10.1164\/rccm.201001-0094OC","volume":"182","author":"M Esperatti","year":"2010","unstructured":"Esperatti, M. et al. 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