{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:04:04Z","timestamp":1773878644893,"version":"3.50.1"},"reference-count":50,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2018,9,20]],"date-time":"2018-09-20T00:00:00Z","timestamp":1537401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2018,9,20]],"date-time":"2018-09-20T00:00:00Z","timestamp":1537401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The oncogenic <jats:italic>gammaherpesviruses<\/jats:italic>, Epstein\u2013Barr virus (EBV) and Kaposi\u2019s sarcoma herpesvirus (KSHV), are etiologically associated with a variety of human cancers, including Burkitt\u2019s lymphoma (BL), Hodgkin lymphoma (HL), Kaposi\u2019s sarcoma (KS), and primary effusion lymphoma (PEL). Recently, we demonstrated KSHV infection of B- and endothelial cells to significantly upregulate the expression of interferon induced transmembrane protein 1 (IFITM1) which in turn enhances virus entry. This is an extension of the above study. In here, we determined EBV infection of cells to trigger IFITM1 expression, <jats:italic>in vitro<\/jats:italic>. Silencing IFITM1 expression using siRNA specifically lowered <jats:italic>gammaherpesvirus<\/jats:italic> infection of cells at a post binding stage of entry. A natural model system to explore the effect of IFITM1 on <jats:italic>gammaherpesvirus<\/jats:italic> infection <jats:italic>in vivo<\/jats:italic> is infection of BALB\/c mice with murine <jats:italic>gammaherpesvirus<\/jats:italic> 68 (MHV-68). Priming mice with siRNA specific to IFITM1 significantly lowered MHV-68 titers in the lung specimens compared to priming with (NS)siRNA or PBS. MHV-68 titers were monitored by plaque assay and qPCR. Taken together, for the first time, this study provides insight into the critical role of IFITM1 to promoting <jats:italic>in vivo gammaherpesvirus<\/jats:italic> infections.<\/jats:p>","DOI":"10.1038\/s41598-018-32350-0","type":"journal-article","created":{"date-parts":[[2018,9,14]],"date-time":"2018-09-14T10:37:51Z","timestamp":1536921471000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["IFITM1 expression is crucial to gammaherpesvirus infection, in vivo"],"prefix":"10.1038","volume":"8","author":[{"given":"Hosni A. M.","family":"Hussein","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarina","family":"Briestenska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jela","family":"Mistrikova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaw M.","family":"Akula","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,9,20]]},"reference":[{"key":"32350_CR1","doi-asserted-by":"publisher","first-page":"1865","DOI":"10.1126\/science.7997879","volume":"266","author":"Y Chang","year":"1994","unstructured":"Chang, Y. et al. Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi\u2019s sarcoma. Science 266, 1865\u20131869 (1994).","journal-title":"Science"},{"key":"32350_CR2","doi-asserted-by":"publisher","first-page":"1837","DOI":"10.1126\/science.282.5395.1837","volume":"282","author":"RC Gallo","year":"1998","unstructured":"Gallo, R. C. The enigmas of Kaposi\u2019s sarcoma. Science 282, 1837\u20131839 (1998).","journal-title":"Science"},{"key":"32350_CR3","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1038\/sj.leu.2403532","volume":"19","author":"KE Hamden","year":"2005","unstructured":"Hamden, K. E. et al. Raf and VEGF: emerging therapeutic targets in Kaposi\u2019s sarcoma-associated herpesvirus infection and angiogenesis in hematopoietic and nonhematopoietic tumors. Leukemia 19, 18\u201326 (2005).","journal-title":"Leukemia"},{"key":"32350_CR4","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1006\/scbi.1999.0129","volume":"9","author":"F Neipel","year":"1999","unstructured":"Neipel, F. & Fleckenstein, B. The role of HHV-8 in Kaposi\u2019s sarcoma. Semin Cancer Biol 9, 151\u2013164 (1999).","journal-title":"Semin Cancer Biol"},{"key":"32350_CR5","doi-asserted-by":"publisher","first-page":"4258","DOI":"10.3390\/v6114258","volume":"6","author":"Y Chang","year":"2014","unstructured":"Chang, Y. & Moore, P. Twenty years of KSHV. Viruses 6, 4258\u20134264 (2014).","journal-title":"Viruses"},{"key":"32350_CR6","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1006\/viro.2000.0851","volume":"282","author":"SM Akula","year":"2001","unstructured":"Akula, S. M., Wang, F. Z., Vieira, J. & Chandran, B. Human herpesvirus 8 interaction with target cells involves heparan sulfate. Virology 282, 245\u2013255 (2001).","journal-title":"Virology"},{"key":"32350_CR7","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1006\/viro.2001.0921","volume":"284","author":"SM Akula","year":"2001","unstructured":"Akula, S. M., Pramod, N. P., Wang, F. Z. & Chandran, B. Human herpesvirus 8 envelope-associated glycoprotein B interacts with heparan sulfate-like moieties. Virology 284, 235\u2013249 (2001).","journal-title":"Virology"},{"key":"32350_CR8","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1016\/S0092-8674(02)00628-1","volume":"108","author":"SM Akula","year":"2002","unstructured":"Akula, S. M., Pramod, N. P., Wang, F. Z. & Chandran, B. Integrin alpha3beta1 (CD 49c\/29) is a cellular receptor for Kaposi\u2019s sarcoma-associated herpesvirus (KSHV\/HHV-8) entry into the target cells. Cell 108, 407\u2013419 (2002).","journal-title":"Cell"},{"key":"32350_CR9","doi-asserted-by":"publisher","first-page":"961","DOI":"10.1038\/nm.2805","volume":"18","author":"AS Hahn","year":"2012","unstructured":"Hahn, A. S. et al. The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi\u2019s sarcoma-associated herpesvirus. Nat Med 18, 961\u2013966 (2012).","journal-title":"Nat Med"},{"key":"32350_CR10","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-18225-w","volume":"7","author":"HAM Hussein","year":"2017","unstructured":"Hussein, H. A. M. & Akula, S. M. miRNA-36 inhibits KSHV, EBV, HSV-2 infection of cells via stifling expression of interferon induced transmembrane protein 1 (IFITM1). Sci Rep. 7, 17972 (2017).","journal-title":"Sci Rep."},{"key":"32350_CR11","doi-asserted-by":"publisher","first-page":"6891","DOI":"10.1093\/emboj\/19.24.6891","volume":"19","author":"J Guo","year":"2000","unstructured":"Guo, J., Hui, D. J., Merrick, W. C. & Sen, G. C. A new pathway of translational regulation mediated by eukaryotic initiation factor 3. Embo j 19, 6891\u20136899 (2000).","journal-title":"Embo j"},{"key":"32350_CR12","doi-asserted-by":"publisher","first-page":"2126","DOI":"10.1128\/JVI.01531-10","volume":"85","author":"J Lu","year":"2011","unstructured":"Lu, J. et al. The IFITM proteins inhibit HIV-1 infection. J Virol 85, 2126\u20132137 (2011).","journal-title":"J Virol"},{"key":"32350_CR13","doi-asserted-by":"publisher","first-page":"e96579","DOI":"10.1371\/journal.pone.0096579","volume":"9","author":"CJ Warren","year":"2014","unstructured":"Warren, C. J. et al. The antiviral restriction factors IFITM1, 2 and 3 do not inhibit infection of human papillomavirus, cytomegalovirus and adenovirus. PLoS One 9, e96579 (2014).","journal-title":"PLoS One"},{"key":"32350_CR14","doi-asserted-by":"publisher","first-page":"6756","DOI":"10.1073\/pnas.1320856111","volume":"111","author":"X Zhao","year":"2014","unstructured":"Zhao, X. et al. Interferon induction of IFITM proteins promotes infection by human coronavirus OC43. Proc Natl Acad Sci USA 111, 6756\u20136761 (2014).","journal-title":"Proc Natl Acad Sci USA"},{"key":"32350_CR15","doi-asserted-by":"publisher","first-page":"e1002292","DOI":"10.1371\/journal.ppat.1002292","volume":"7","author":"RS Leang","year":"2011","unstructured":"Leang, R. S. et al. The anti-interferon activity of conserved viral dUTPase ORF54 is essential for an effective MHV-68 infection. PLoS Pathog 7, e1002292 (2011).","journal-title":"PLoS Pathog"},{"key":"32350_CR16","doi-asserted-by":"publisher","first-page":"e11672","DOI":"10.1371\/journal.pone.0011672","volume":"5","author":"B Steer","year":"2010","unstructured":"Steer, B., Adler, B., Jonjic, S., Stewart, J. P. & Adler, H. A gammaherpesvirus complement regulatory protein promotes initiation of infection by activation of protein kinase Akt\/PKB. PLoS One 5, e11672 (2010).","journal-title":"PLoS One"},{"key":"32350_CR17","doi-asserted-by":"publisher","first-page":"2373","DOI":"10.4049\/jimmunol.1400486","volume":"193","author":"LT Donlin","year":"2014","unstructured":"Donlin, L. T., Jayatilleke, A., Giannopoulou, E. G., Kalliolias, G. D. & Ivashkiv, L. B. Modulation of TNF-induced macrophage polarization by synovial fibroblasts. J Immunol 193, 2373\u20132383 (2014).","journal-title":"J Immunol"},{"key":"32350_CR18","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1038\/nri3581","volume":"14","author":"LB Ivashkiv","year":"2014","unstructured":"Ivashkiv, L. B. & Donlin, L. T. Regulation of type I interferon responses. Nat Rev Immunol 14, 36\u201349 (2014).","journal-title":"Nat Rev Immunol"},{"key":"32350_CR19","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1146\/annurev-immunol-032713-120231","volume":"32","author":"WM Schneider","year":"2014","unstructured":"Schneider, W. M., Chevillotte, M. D. & Rice, C. M. Interferon-stimulated genes: a complex web of host defenses. Annu Rev Immunol 32, 513\u2013545 (2014).","journal-title":"Annu Rev Immunol"},{"key":"32350_CR20","doi-asserted-by":"publisher","first-page":"12646","DOI":"10.1128\/JVI.01328-10","volume":"84","author":"JM Weidner","year":"2010","unstructured":"Weidner, J. M. et al. Interferon-induced cell membrane proteins, IFITM3 and tetherin, inhibit vesicular stomatitis virus infection via distinct mechanisms. J Virol 84, 12646\u201312657 (2010).","journal-title":"J Virol"},{"key":"32350_CR21","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-13-155","volume":"13","author":"D Hickford","year":"2012","unstructured":"Hickford, D., Frankenberg, S., Shaw, G. & Renfree, M. B. Evolution of vertebrate interferon inducible transmembrane proteins. BMC Genomics 13, 155 (2012).","journal-title":"BMC Genomics"},{"key":"32350_CR22","doi-asserted-by":"publisher","first-page":"840","DOI":"10.1073\/pnas.86.3.840","volume":"86","author":"LE Reid","year":"1989","unstructured":"Reid, L. E. et al. A single DNA response element can confer inducibility by both alpha- and gamma-interferons. Proc Natl Acad Sci USA 86, 840\u2013844 (1989).","journal-title":"Proc Natl Acad Sci USA"},{"key":"32350_CR23","doi-asserted-by":"publisher","first-page":"1243","DOI":"10.1016\/j.cell.2009.12.017","volume":"139","author":"AL Brass","year":"2009","unstructured":"Brass, A. L. et al. The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus. Cell 139, 1243\u20131254 (2009).","journal-title":"Cell"},{"key":"32350_CR24","doi-asserted-by":"publisher","first-page":"23860","DOI":"10.1074\/jbc.270.40.23860","volume":"270","author":"GA Deblandre","year":"1995","unstructured":"Deblandre, G. A. et al. Expression cloning of an interferon-inducible 17-kDa membrane protein implicated in the control of cell growth. J Biol Chem 270, 23860\u201323866 (1995).","journal-title":"J Biol Chem"},{"key":"32350_CR25","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.celrep.2015.08.055","volume":"13","author":"J Yu","year":"2015","unstructured":"Yu, J. et al. IFITM Proteins Restrict HIV-1 Infection by Antagonizing the Envelope Glycoprotein. Cell Rep 13, 145\u2013156 (2015).","journal-title":"Cell Rep"},{"key":"32350_CR26","doi-asserted-by":"publisher","first-page":"25946","DOI":"10.1074\/jbc.M115.657346","volume":"290","author":"SK Narayana","year":"2015","unstructured":"Narayana, S. K. et al. The Interferon-induced Transmembrane Proteins, IFITM1, IFITM2, and IFITM3 Inhibit Hepatitis C Virus Entry. J Biol Chem 290, 25946\u201325959 (2015).","journal-title":"J Biol Chem"},{"key":"32350_CR27","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.virol.2016.04.002","volume":"494","author":"LS Chesnokova","year":"2016","unstructured":"Chesnokova, L. S., Valencia, S. M. & Hutt-Fletcher, L. M. The BDLF3 gene product of Epstein-Barr virus, gp150, mediates non-productive binding to heparan sulfate on epithelial cells and only the binding domain of CD21 is required for infection. Virology 494, 23\u201328 (2016).","journal-title":"Virology"},{"key":"32350_CR28","doi-asserted-by":"publisher","first-page":"12591","DOI":"10.1128\/JVI.01167-08","volume":"82","author":"N Jarousse","year":"2008","unstructured":"Jarousse, N., Chandran, B. & Coscoy, L. Lack of heparan sulfate expression in B-cell lines: implications for Kaposi\u2019s sarcoma-associated herpesvirus and murine gammaherpesvirus 68 infections. J Virol 82, 12591\u201312597 (2008).","journal-title":"J Virol"},{"key":"32350_CR29","doi-asserted-by":"publisher","first-page":"e0175053","DOI":"10.1371\/journal.pone.0175053","volume":"12","author":"Y Torii","year":"2017","unstructured":"Torii, Y. et al. Epstein-Barr virus infection-induced inflammasome activation in human monocytes. PLoS One 12, e0175053 (2017).","journal-title":"PLoS One"},{"key":"32350_CR30","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1002\/ijc.2910170203","volume":"17","author":"KO Fresen","year":"1976","unstructured":"Fresen, K. O. & Hausen, H. Establishment of EBNA-expressing cell lines by infection of Epstein-Barr virus (EBV)-genome-negative human lymphoma cells with different EBV strains. Int J Cancer 17, 161\u2013166 (1976).","journal-title":"Int J Cancer"},{"key":"32350_CR31","first-page":"468","volume":"24","author":"D Blaskovic","year":"1980","unstructured":"Blaskovic, D., Stancekova, M., Svobodova, J. & Mistrikova, J. Isolation of five strains of herpesviruses from two species of free living small rodents. Acta virologica 24, 468 (1980).","journal-title":"Acta virologica"},{"key":"32350_CR32","doi-asserted-by":"publisher","first-page":"105","DOI":"10.4149\/av_2013_02_105","volume":"57","author":"J Cipkova-Jarcuskova","year":"2013","unstructured":"Cipkova-Jarcuskova, J., Chalupkova, A., Hrabovska, Z., Wagnerova, M. & Mistrikova, J. Biological and pathogenetic characterization of different isolates of murine gammaherpesvirus 68 (MHV-68) in the context of study of human oncogenic gammaherpesviruses. Acta virologica 57, 105\u2013112 (2013).","journal-title":"Acta virologica"},{"key":"32350_CR33","doi-asserted-by":"publisher","first-page":"1074","DOI":"10.4049\/jimmunol.165.2.1074","volume":"165","author":"E Flano","year":"2000","unstructured":"Flano, E., Husain, S. M., Sample, J. T., Woodland, D. L. & Blackman, M. A. Latent murine gamma-herpesvirus infection is established in activated B cells, dendritic cells, and macrophages. J Immunol 165, 1074\u20131081 (2000).","journal-title":"J Immunol"},{"key":"32350_CR34","doi-asserted-by":"publisher","first-page":"e1003124","DOI":"10.1371\/journal.ppat.1003124","volume":"9","author":"K Li","year":"2013","unstructured":"Li, K. et al. IFITM proteins restrict viral membrane hemifusion. PLoS Pathog 9, e1003124 (2013).","journal-title":"PLoS Pathog"},{"key":"32350_CR35","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1016\/j.chom.2013.03.006","volume":"13","author":"S Amini-Bavil-Olyaee","year":"2013","unstructured":"Amini-Bavil-Olyaee, S. et al. The antiviral effector IFITM3 disrupts intracellular cholesterol homeostasis to block viral entry. Cell Host Microbe 13, 452\u2013464 (2013).","journal-title":"Cell Host Microbe"},{"key":"32350_CR36","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.canlet.2017.04.005","volume":"399","author":"AJ Lui","year":"2017","unstructured":"Lui, A. J. et al. IFITM1 suppression blocks proliferation and invasion of aromatase inhibitor-resistant breast cancer in vivo by JAK\/STAT-mediated induction of p21. Cancer Lett 399, 29\u201343 (2017).","journal-title":"Cancer Lett"},{"key":"32350_CR37","doi-asserted-by":"publisher","DOI":"10.1186\/s40364-016-0064-5","volume":"4","author":"D Borg","year":"2016","unstructured":"Borg, D. et al. Expression of IFITM1 as a prognostic biomarker in resected gastric and esophageal adenocarcinoma. Biomark Res 4, 10 (2016).","journal-title":"Biomark Res"},{"key":"32350_CR38","doi-asserted-by":"publisher","first-page":"e85811","DOI":"10.1172\/jci.insight.85811","volume":"2","author":"RA Raposo","year":"2017","unstructured":"Raposo, R. A. et al. IFITM1 targets HIV-1 latently infected cells for antibody-dependent cytolysis. JCI insight 2, e85811 (2017).","journal-title":"JCI insight"},{"key":"32350_CR39","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1002\/hep.26066","volume":"57","author":"C Wilkins","year":"2013","unstructured":"Wilkins, C. et al. IFITM1 is a tight junction protein that inhibits hepatitis C virus entry. Hepatology 57, 461\u2013469 (2013).","journal-title":"Hepatology"},{"key":"32350_CR40","doi-asserted-by":"publisher","first-page":"4516","DOI":"10.1182\/blood-2004-09-3683","volume":"105","author":"SM Akula","year":"2005","unstructured":"Akula, S. M. et al. B-Raf-dependent expression of vascular endothelial growth factor-A in Kaposi sarcoma-associated herpesvirus-infected human B cells. Blood 105, 4516\u20134522 (2005).","journal-title":"Blood"},{"key":"32350_CR41","doi-asserted-by":"publisher","first-page":"37491","DOI":"10.1074\/jbc.M110.159103","volume":"285","author":"OF Dyson","year":"2010","unstructured":"Dyson, O. F., Traylen, C. M. & Akula, S. M. Cell membrane-bound Kaposi\u2019s sarcoma-associated herpesvirus-encoded glycoprotein B promotes virus latency by regulating expression of cellular Egr-1. J Biol Chem 285, 37491\u201337502 (2010).","journal-title":"J Biol Chem"},{"key":"32350_CR42","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/j.virol.2010.03.014","volume":"402","author":"GL Bentz","year":"2010","unstructured":"Bentz, G. L., Liu, R., Hahn, A. M., Shackelford, J. & Pagano, J. S. Epstein-Barr virus BRLF1 inhibits transcription of IRF3 and IRF7 and suppresses induction of interferon-beta. Virology 402, 121\u2013128 (2010).","journal-title":"Virology"},{"key":"32350_CR43","doi-asserted-by":"publisher","first-page":"369","DOI":"10.4149\/av_2015_04_369","volume":"59","author":"L Ancicova","year":"2015","unstructured":"Ancicova, L. et al. Simultaneous infection with gammaherpes and influenza viruses enhances the host immune defense. Acta virologica 59, 369\u2013379 (2015).","journal-title":"Acta virologica"},{"key":"32350_CR44","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13578-015-0066-2","volume":"6","author":"LR Walker","year":"2016","unstructured":"Walker, L. R., Hussein, H. A. & Akula, S. M. Subcellular fractionation method to study endosomal trafficking of Kaposi\u2019s sarcoma-associated herpesvirus. Cell & bioscience 6, 1 (2016).","journal-title":"Cell & bioscience"},{"key":"32350_CR45","doi-asserted-by":"crossref","unstructured":"Walker, L.R., Hussein, H.A. & Akula, S.M. Disintegrin-like domain of glycoprotein B regulates Kaposi\u2019s sarcoma-associated herpesvirus infection of cells. J Gen Virol (2014).","DOI":"10.1099\/vir.0.066829-0"},{"key":"32350_CR46","doi-asserted-by":"publisher","first-page":"8940","DOI":"10.1128\/JVI.00569-11","volume":"85","author":"AR Robinson","year":"2011","unstructured":"Robinson, A. R., Kwek, S. S., Hagemeier, S. R., Wille, C. K. & Kenney, S. C. Cellular transcription factor Oct-1 interacts with the Epstein-Barr virus BRLF1 protein to promote disruption of viral latency. J Virol 85, 8940\u20138953 (2011).","journal-title":"J Virol"},{"key":"32350_CR47","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1016\/0042-6822(64)90204-1","volume":"24","author":"DH Watson","year":"1964","unstructured":"Watson, D. H., Wildy, P. & Russell, W. C. Quantitative Electron Microscopy Studies On the Growth of Herpes Virus Using the Techniques of Negative Staining and Ultramicrotomy. Virology 24, 523\u2013538 (1964).","journal-title":"Virology"},{"key":"32350_CR48","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1038\/nprot.2008.73","volume":"3","author":"TD Schmittgen","year":"2008","unstructured":"Schmittgen, T. D. & Livak, K. J. Analyzing real-time PCR data by the comparative C(T) method. Nature protocols 3, 1101\u20131108 (2008).","journal-title":"Nature protocols"},{"key":"32350_CR49","doi-asserted-by":"publisher","first-page":"55","DOI":"10.4149\/av_2011_01_55","volume":"55","author":"V Stiglincova","year":"2011","unstructured":"Stiglincova, V. et al. Vertical transmission of murine gammaherpesvirus 68 in mice. Acta virologica 55, 55\u201359 (2011).","journal-title":"Acta virologica"},{"key":"32350_CR50","doi-asserted-by":"publisher","first-page":"458","DOI":"10.3389\/fcimb.2017.00458","volume":"7","author":"V Hajnicka","year":"2017","unstructured":"Hajnicka, V. et al. Tick-Borne Transmission of Murine Gammaherpesvirus 68. Frontiers in cellular and infection microbiology 7, 458 (2017).","journal-title":"Frontiers in cellular and infection microbiology"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-018-32350-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-018-32350-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-018-32350-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T09:42:30Z","timestamp":1671615750000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-018-32350-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,20]]},"references-count":50,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2018,12]]}},"alternative-id":["32350"],"URL":"https:\/\/doi.org\/10.1038\/s41598-018-32350-0","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,20]]},"assertion":[{"value":"4 May 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 September 2018","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 September 2018","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"14105"}}