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Spinosad is considered an eco-friendly insecticide used for the management of<jats:italic>M<\/jats:italic>.<jats:italic>domestica<\/jats:italic>and other pests of significant concern. Cases of resistance against spinosad in<jats:italic>M<\/jats:italic>.<jats:italic>domestica<\/jats:italic>have been reported from some parts of the world; however, there are no reports of any negative effects of spinosad resistance on the fitness\/biological parameters of<jats:italic>M<\/jats:italic>.<jats:italic>domestica<\/jats:italic>. To investigate fitness costs, a near isogenic<jats:italic>M<\/jats:italic>.<jats:italic>domestica<\/jats:italic>resistant strain (Spin-R) was constructed using Spin-UNSEL-susceptible and Spin-SEL-resistant strains sharing a close genetic background. We found significantly reduced rates of adult eclosion, fecundity, egg hatching, survival, and lengthened developmental time in the Spin-R strain. Moreover, the values of different fitness parameters like biotic potential, mean relative growth rate, intrinsic rate of natural increase, and net reproductive rate, were also significantly reduced in the Spin-R strain, which reflect fitness costs most probably linked with spinosad resistance. The presence of fitness costs suggests likely instability of resistance to spinosad in<jats:italic>M<\/jats:italic>.<jats:italic>domestica<\/jats:italic>, which can be reverted by relaxing spinosad selection pressure and rotation with alternate insecticides. The wise use of insecticides will ultimately help to manage resistance in this pest and minimize environmental pollution.<\/jats:p>","DOI":"10.1038\/s41598-018-32445-8","type":"journal-article","created":{"date-parts":[[2018,9,13]],"date-time":"2018-09-13T09:17:22Z","timestamp":1536830242000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Spinosad resistance affects biological parameters of Musca domestica Linnaeus"],"prefix":"10.1038","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7209-4750","authenticated-orcid":false,"given":"Hafiz Azhar Ali","family":"Khan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,9,19]]},"reference":[{"key":"32445_CR1","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1016\/j.chemosphere.2016.10.018","volume":"167","author":"HAA Khan","year":"2017","unstructured":"Khan, H. 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