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However, accurate characterization of these analytes requires rigorous evaluation of cell-to-cell heterogeneity in fluorescence intensities and intracellular distribution of probes. In this study, we perform a parallel and systematic comparison of two small-molecule fluorescent vesicular Zn\n                    <jats:sup>2+<\/jats:sup>\n                    probes, FluoZin-3 AM and SpiroZin2, to evaluate each probe for measurement of vesicular Zn\n                    <jats:sup>2+<\/jats:sup>\n                    pools. Our results reveal that SpiroZin2 is a specific lysosomal vesicular Zn\n                    <jats:sup>2+<\/jats:sup>\n                    probe and affords uniform measurement of resting Zn\n                    <jats:sup>2+<\/jats:sup>\n                    levels at the single cell level with proper calibration. In contrast, FluoZin-3 AM produces highly variable fluorescence intensities and non-specifically localizes in the cytosol and multiple vesicular compartments. We further applied SpiroZin2 to lactating mouse mammary epithelial cells and detected a transient increase of lysosomal free Zn\n                    <jats:sup>2+<\/jats:sup>\n                    at 24-hour after lactation hormone treatment, which implies that lysosomes play a role in the regulation of Zn\n                    <jats:sup>2+<\/jats:sup>\n                    homeostasis during lactation. This study demonstrates the need for critical characterization of small-molecule fluorescent probes to define the concentration and localization of analytes in different cell populations, and reveals SpiroZin2 to be capable of reporting diverse perturbations to lysosomal Zn\n                    <jats:sup>2+<\/jats:sup>\n                    .\n                  <\/jats:p>","DOI":"10.1038\/s41598-018-33102-w","type":"journal-article","created":{"date-parts":[[2018,10,3]],"date-time":"2018-10-03T08:03:02Z","timestamp":1538553782000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Superiority of SpiroZin2 Versus FluoZin-3 for monitoring vesicular Zn2+ allows tracking of lysosomal Zn2+ pools"],"prefix":"10.1038","volume":"8","author":[{"given":"Yu","family":"Han","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8004-3769","authenticated-orcid":false,"given":"Jacob M.","family":"Goldberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J.","family":"Lippard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5794-5983","authenticated-orcid":false,"given":"Amy E.","family":"Palmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,10,9]]},"reference":[{"key":"33102_CR1","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1152\/physrev.00035.2014","volume":"95","author":"T Kambe","year":"2015","unstructured":"Kambe, T., Tsuji, T., Hashimoto, A. & Itsumura, N. 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