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We reveal that SPMs, but not LPMs, have the ability to present antigens to na\u00efve CD4<jats:sup>+<\/jats:sup>T cells. Coculture of SPMs with na\u00efve ovalbumin (OVA) specific CD4<jats:sup>+<\/jats:sup>T cells (OT-II) in the presence of OVA peptide effectively induced CD4<jats:sup>+<\/jats:sup>T cells priming. SPMs, but not LPMs, strongly express DNAM-1, an activating immunoreceptor. Although antigen uptake and processing were comparable between WT and DNAM-1-deficient SPMs, deficiency of DNAM-1 on SPMs or blockade of DNAM-1 and its ligand interaction impaired CD4<jats:sup>+<\/jats:sup>T cells priming by SPMs. Furthermore, T and B cell responses in mediastinal lymph nodes of mice intraperitoneally immunized with trinitrophenyl (TNP)\u2013OVA protein in Alum adjuvant were enhanced by intraperitoneally transferred wild-type, but not DNAM-1-deficient, SPMs. We propose that SPMs are functionally distinct from LPMs, and DNAM-1 plays a costimulatory role in antigen presentation by SPMs.<\/jats:p>","DOI":"10.1038\/s41598-018-33437-4","type":"journal-article","created":{"date-parts":[[2018,10,8]],"date-time":"2018-10-08T12:46:46Z","timestamp":1539002806000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Selective DNAM-1 expression on small peritoneal macrophages contributes to CD4+ T cell costimulation"],"prefix":"10.1038","volume":"8","author":[{"given":"Eri","family":"Takenaka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anh","family":"Van Vo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumi","family":"Yamashita-Kanemaru","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Shibuya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuko","family":"Shibuya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,10,12]]},"reference":[{"key":"33437_CR1","doi-asserted-by":"publisher","first-page":"986","DOI":"10.1038\/ni.2705","volume":"14","author":"LC Davies","year":"2013","unstructured":"Davies, L. 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