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Intercostal muscle ultrasound offers a repeatable and radiation-free alternative, however requires validation. We aimed to determine the reliability of parasternal intercostal muscle ultrasound, and the concurrent validity of parasternal ultrasound with clinicometric parameters. Twenty stable COPD patients underwent ultrasound measurement of thickness and echogenicity of 2<jats:sup>nd<\/jats:sup> and 3<jats:sup>rd<\/jats:sup> parasternal intercostal muscles, dominant pectoralis major and quadriceps, and diaphragm thickness; spirometry; and chest CT. Intra-rater intraclass correlation (ICC) for ultrasound intercostal thickness was 0.87\u20130.97 depending on site, with echogenicity ICC 0.63\u20130.91. Inter-rater ICC was fair to excellent. Ultrasound intercostal thickness moderately correlated with FEV<jats:sub>1<\/jats:sub>% predicted (r\u2009=\u20090.33) and quadriceps thickness (r\u2009=\u20090.31). Echogenicity correlated negatively with FEV<jats:sub>1<\/jats:sub>% predicted (r\u2009=\u2009\u22120.32). CT-measured lateral intercostal mass correlate negatively with parasternal ultrasound intercostal thickness. These data confirm ultrasound of parasternal intercostal musculature is reproducible. Lower intercostal muscle quantity and quality reflects greater COPD spirometric severity. This novel tool may have biomarker potential for both the systemic effects of COPD on muscle as well as local disruption of respiratory mechanics. The negative correlation between CT and ultrasound measurements may reflect complex site-dependent interactions between respiratory muscles and the chest wall.<\/jats:p>","DOI":"10.1038\/s41598-018-33666-7","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T14:21:18Z","timestamp":1539094878000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Parasternal intercostal muscle ultrasound in chronic obstructive pulmonary disease correlates with spirometric severity"],"prefix":"10.1038","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8506-6233","authenticated-orcid":false,"given":"Peter","family":"Wallbridge","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selina M.","family":"Parry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sourav","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Candice","family":"Law","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Hammerschlag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Irving","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Hew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Steinfort","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,10,15]]},"reference":[{"key":"33666_CR1","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1111\/resp.13012","volume":"22","author":"CF Vogelmeier","year":"2017","unstructured":"Vogelmeier, C. 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