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PDGFR-\u03b2 siRNA-PEI-PLGA-PEG NP significantly induced autophagy in human vaginal epithelial cells (VK2\/E6E7) 48\u2009hr post treatment by improving autophagic degradation activity without causing inflammation, apoptosis or any decrease in cell viability. Beclin-1, VPS34 (markers for initiation stage of autophagy), UVRAG, TECPR-1 (markers for degradation stage of autophagy) were found to be significantly upregulated after treatment with PDGFR-\u03b2 siRNA-PEI-PLGA-PEG NP. Furthermore, PDGFR-\u03b2 siRNA-PEI-PLGA-PEG NP decreased PDGFR-\u03b2 mRNA expression by 50% and protein expression by 43% in VK2\/E6E7 cells 48\u2009hr post treatment. Treatment of cells with PDGFR-\u03b2 siRNA-PEI-PLGA-PEG NP significantly decreased the intracellular C. trachomatis and extracellular release of C. trachomatis by approximately 65% and 67%, respectively, <jats:italic>in vitro<\/jats:italic> through augmenting autophagic degradation pathways and reducing bacterial binding simultaneously.<\/jats:p>","DOI":"10.1038\/s41598-018-36601-y","type":"journal-article","created":{"date-parts":[[2019,2,4]],"date-time":"2019-02-04T11:04:09Z","timestamp":1549278249000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Autophagy induction and PDGFR-\u03b2 knockdown by siRNA-encapsulated nanoparticles reduce chlamydia trachomatis infection"],"prefix":"10.1038","volume":"9","author":[{"given":"Sidi","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yannick","family":"Traore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celine","family":"Jimenez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel A.","family":"Ho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,2,4]]},"reference":[{"key":"36601_CR1","first-page":"303","volume":"138","author":"M Malhotra","year":"2013","unstructured":"Malhotra, M., Sood, S., Mukherjee, A., Muralidhar, S. & Bala, M. 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