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Transcriptome analysis demonstrated for the first time the upregulation of 115 and downregulation of 131 genes in CD271-MSCs. Functional enrichment analysis showed that the upregulated genes in CD271-MSCs are significantly enriched for extracellular matrix (<jats:italic>tenascin XB, elastin, ABI family, member 3 (NESH) binding protein, carboxypeptidase Z, laminin alpha 2<\/jats:italic>and<jats:italic>nephroblastoma overexpressed<\/jats:italic>) and cell adhesion (<jats:italic>CXCR7, GPNMB, MYBPH, SVEP1, ARHGAP6, TSPEAR, PIK3CG, ABL2<\/jats:italic>and<jats:italic>NCAM1<\/jats:italic>). CD271-MSCs expressed higher gene transcript levels that are involved in early osteogenesis\/chondrogenesis\/adipogenesis (<jats:italic>ZNF145, FKBP5<\/jats:italic>). In addition, increased transcript levels for early and late osteogenesis (<jats:italic>DPT, OMD, ID4, CRYAB, SORT1<\/jats:italic>), adipogenesis (<jats:italic>CTNNB1, ZEB, LPL, FABP4, PDK4, ACDC<\/jats:italic>), and chondrogenesis (<jats:italic>CCN3\/NOV, CCN4\/WISP1, CCN5\/WISP2 and ADAMTS-5<\/jats:italic>) were detected. Interestingly, CD271-MSCs expressed increased levels of hematopoiesis associated genes (<jats:italic>CXCL12, FLT3L, IL-3, TPO, KITL<\/jats:italic>). Down-regulated genes in CD271-MSCs were associated with WNT and TGF-beta signaling, and cytokine\/chemokine signaling pathways. In addition to their capacity to support hematopoiesis, these results suggest that CD271-MSCs may contain more osteo\/chondro progenitors and\/or feature a greater differentiation potential.<\/jats:p>","DOI":"10.1038\/s41598-019-38517-7","type":"journal-article","created":{"date-parts":[[2019,2,11]],"date-time":"2019-02-11T11:03:54Z","timestamp":1549883034000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Molecular signature of human bone marrow-derived mesenchymal stromal cell subsets"],"prefix":"10.1038","volume":"9","author":[{"given":"Selim","family":"Ku\u00e7i","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zyrafete","family":"Ku\u00e7i","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Sch\u00e4fer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Spohn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Winter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Schwab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilia","family":"Salzmann-Manrique","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Klingebiel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Bader","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,2,11]]},"reference":[{"key":"38517_CR1","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1126\/science.284.5411.143","volume":"284","author":"MF Pittenger","year":"1999","unstructured":"Pittenger, M. 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