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The direct measurement of NO is challenging due to its short half-life, and as such surrogate measurements are typically used to approximate its relative concentrations. Here we demonstrate that ruthenium-based [Ru(bpy)<jats:sub>2<\/jats:sub>(dabpy)]<jats:sup>2+<\/jats:sup> is a potent sensor for NO in its irreversible, NO-bound active form, [Ru(bpy)<jats:sub>2<\/jats:sub>(T-bpy)]<jats:sup>2+<\/jats:sup>. Using spectrophotometry we established the sensor\u2019s ability to detect and measure soluble NO in a concentration-dependent manner in cell-free media. Endothelial cells cultured with acetylcholine or hydrogen peroxide to induce endogenous NO production showed modest increases of 7.3\u2009\u00b1\u20097.1% and 36.3\u2009\u00b1\u200925.0% respectively in fluorescence signal from baseline state, while addition of exogenous NO increased their fluorescence by 5.2-fold. The changes in fluorescence signal were proportionate and comparable against conventional NO assays. Rabbit blood samples immediately exposed to [Ru(bpy)<jats:sub>2<\/jats:sub>(dabpy)]<jats:sup>2+<\/jats:sup> displayed 8-fold higher mean fluorescence, relative to blood without sensor. Approximately 14% of the observed signal was NO\/NO adduct-specific. Optimal readings were obtained when sensor was added to freshly collected blood, remaining stable during subsequent freeze-thaw cycles. Clinical studies are now required to test the utility of [Ru(bpy)<jats:sub>2<\/jats:sub>(dabpy)]<jats:sup>2+<\/jats:sup> as a sensor to detect changes in NO from human blood samples in cardiovascular health and disease.<\/jats:p>","DOI":"10.1038\/s41598-019-39123-3","type":"journal-article","created":{"date-parts":[[2019,2,8]],"date-time":"2019-02-08T13:55:25Z","timestamp":1549634125000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A Novel Ruthenium-based Molecular Sensor to Detect Endothelial Nitric Oxide"],"prefix":"10.1038","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0126-799X","authenticated-orcid":false,"given":"Achini K.","family":"Vidanapathirana","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin J.","family":"Pullen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0943-824X","authenticated-orcid":false,"given":"Run","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MyNgan","family":"Duong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarrad M.","family":"Goyne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaozhou","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9875-967X","authenticated-orcid":false,"given":"Claudine S.","family":"Bonder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew D.","family":"Abell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina A.","family":"Bursill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J.","family":"Nicholls","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Psaltis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,2,8]]},"reference":[{"key":"39123_CR1","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.freeradbiomed.2008.03.020","volume":"45","author":"DD Thomas","year":"2008","unstructured":"Thomas, D. 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