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However, its biological role in the disease is unclear. Our objective was to determine the expression pattern and biological role of <jats:italic>PTCH1<\/jats:italic> in the lungs of patients with COPD. Airway epithelial-specific PTCH1 protein expression and epithelial morphology were assessed in lung tissues of control and COPD patients. <jats:italic>PTCH1<\/jats:italic> mRNA expression was measured in bronchial epithelial cells obtained from individuals with and without COPD. The effects of <jats:italic>PTCH1<\/jats:italic> siRNA knockdown on epithelial repair and mucous expression were evaluated using human epithelial cell lines. <jats:italic>Ptch1<\/jats:italic><jats:sup>+\/\u2212<\/jats:sup> mice were used to assess the effect of decreased PTCH1 on mucous expression and airway epithelial phenotypes. Airway epithelial-specific PTCH1 protein expression was significantly increased in subjects with COPD compared to controls, and its expression was associated with total airway epithelial cell count and thickness. <jats:italic>PTCH1<\/jats:italic> knockdown attenuated wound closure and mucous expression in airway epithelial cell lines. <jats:italic>Ptch1<\/jats:italic><jats:sup>+\/\u2212<\/jats:sup> mice had reduced mucous expression compared to wildtype mice following mucous induction. PTCH1 protein is up-regulated in COPD airway epithelium and may upregulate mucous expression. PTCH1 provides a novel target to reduce chronic bronchitis in COPD patients.<\/jats:p>","DOI":"10.1038\/s41598-019-40045-3","type":"journal-article","created":{"date-parts":[[2019,3,4]],"date-time":"2019-03-04T11:03:46Z","timestamp":1551697426000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Hedgehog signaling in the airway epithelium of patients with chronic obstructive pulmonary disease"],"prefix":"10.1038","volume":"9","author":[{"given":"A.","family":"Tam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7925-6070","authenticated-orcid":false,"given":"M.","family":"Hughes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4737-3499","authenticated-orcid":false,"given":"K. 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