{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T21:01:26Z","timestamp":1790542886910,"version":"4.1.0"},"reference-count":48,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Viral gastroenteritis causes high morbidity worldwide. In this study, stool samples from 179 children aged 0\u20136 years attending Danish day care centers were investigated for gastrointestinal viruses. Each child was observed for one year with submission of samples and questionnaires\u00a0every two months. Adenovirus, norovirus, rotavirus, and sapovirus were detected in samples using real-time PCR. A total of 229 (33%) of the 688 samples collected tested positive for at least one virus. At the first sampling point, adenovirus was shed by 6%, norovirus genotype I by 3% and genotype II by 12%, rotavirus A by 9%, and sapovirus by 21% of the 142 children included in the risk factor analyses. Increasing age was identified as a protective factor against testing positive for gastrointestinal virus, whereas nausea during the previous two months was positively associated with testing positive. Odds of shedding adenovirus were 9.6 times higher among children treated with antibiotics within the previous two months than among children who were not. Gastrointestinal viruses were shed year-round and high viral loads were observed in samples from both symptomatic and asymptomatic children, suggesting children in day care as a reservoir and a possible source of spreading of viruses into the community.<\/jats:p>","DOI":"10.1038\/s41598-019-40077-9","type":"journal-article","created":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T11:06:43Z","timestamp":1551438403000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Children Attending Day Care Centers are a Year-round Reservoir of Gastrointestinal Viruses"],"prefix":"10.1038","volume":"9","author":[{"given":"Betina","family":"Hebbelstrup Jensen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pikka","family":"Jokelainen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex Christian Yde","family":"Nielsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina Tr\u00e6holt","family":"Franck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorthe","family":"Rejkj\u00e6r Holm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristian","family":"Sch\u00f8nning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas M.","family":"Petersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7536-3453","authenticated-orcid":false,"given":"Karen A.","family":"Krogfelt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,3,1]]},"reference":[{"key":"40077_CR1","doi-asserted-by":"publisher","first-page":"1405","DOI":"10.1016\/S0140-6736(13)60222-6","volume":"381","author":"CL Walker","year":"2013","unstructured":"Walker, C. L. et al. Global burden of childhood pneumonia and diarrhoea. Lancet 381, 1405\u20131416 (2013).","journal-title":"Lancet"},{"key":"40077_CR2","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.ijfoodmicro.2014.11.022","volume":"196","author":"MJ Mangen","year":"2011","unstructured":"Mangen, M. J. et al. Cost-of-illness and disease burden of food-related pathogens in the Netherlands, 2011. Int. J. Food Microbiol. 196, 84\u201393 (2011).","journal-title":"Int. J. Food Microbiol."},{"key":"40077_CR3","doi-asserted-by":"publisher","first-page":"1123","DOI":"10.3201\/eid2007.130781","volume":"20","author":"KT Franck","year":"2014","unstructured":"Franck, K. T., Fonager, J., Ersb\u00f8ll, A. K. & B\u00f6ttiger, B. Norovirus Epidemiology in Community and Health Care Settings and Association with Patient Age, Denmark. Emerg. Infect. Dis. 20, 1123\u20131131 (2014).","journal-title":"Emerg. Infect. Dis."},{"key":"40077_CR4","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1007\/s10096-007-0290-8","volume":"26","author":"CF Amar","year":"2007","unstructured":"Amar, C. F. et al. Detection by PCR of eight groups of enteric pathogens in 4,627 faecal samples: re-examination of the English case-control Infectious Intestinal Disease Study (1993\u20131996). Eur. J. Clin. Microbiol. Infect. Dis. 26, 311\u2013323 (2007).","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"40077_CR5","doi-asserted-by":"publisher","first-page":"790","DOI":"10.1093\/infdis\/jit254","volume":"208","author":"P Chhabra","year":"2013","unstructured":"Chhabra, P. et al. Etiology of viral gastroenteritis in children <5 years of age in the United States, 2008\u20132009. J. Infect. Dis. 208, 790\u2013800 (2013).","journal-title":"J. Infect. Dis."},{"key":"40077_CR6","doi-asserted-by":"publisher","first-page":"1663","DOI":"10.1007\/s10096-014-2135-6","volume":"33","author":"MS Corcoran","year":"2014","unstructured":"Corcoran, M. S., van Well, G. T. & van Loo, I. H. Diagnosis of viral gastroenteritis in children: interpretation of real-time PCR results and relation to clinical symptoms. Eur. J. Clin. Microbiol. Infect. Dis. 33, 1663\u20131673 (2014).","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"40077_CR7","doi-asserted-by":"publisher","first-page":"1216","DOI":"10.1086\/651597","volume":"50","author":"HL Kirking","year":"2010","unstructured":"Kirking, H. L. et al. Likely Transmission of Norovirus on an Airplane, October 2008. Clin. Infect. Dis. 50, 1216\u20131221 (2010).","journal-title":"Clin. Infect. Dis."},{"key":"40077_CR8","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1016\/j.jpeds.2008.07.057","volume":"154","author":"WH Lyman","year":"2009","unstructured":"Lyman, W. H. et al. Prospective study of etiologic agents of acute gastroenteritis outbreaks in child care centers. J Pediatr. 154, 253\u2013257 (2009).","journal-title":"J Pediatr."},{"key":"40077_CR9","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1128\/CMR.00011-14","volume":"28","author":"T Oka","year":"2015","unstructured":"Oka, T., Wang, Q., Katayama, K. & Saif, L. J. Comprehensive Review of Human Sapoviruses. Clin. Microbiol. Rev. 28, 32\u201353 (2015).","journal-title":"Clin. Microbiol. Rev."},{"key":"40077_CR10","doi-asserted-by":"publisher","first-page":"1894","DOI":"10.1002\/jmv.24812","volume":"89","author":"JAM Siqueira","year":"2017","unstructured":"Siqueira, J. A. M., J\u00fanior, E. C. S., Linhares, A. D. C. & Gabbay, Y. B. Molecular analysis of norovirus in specimens from children enrolled in a 1982\u20131986 study in Bel\u00e9m, Brazil: A community-based longitudinal study. J. Med. Virol. 89, 1894\u20131903 (2017).","journal-title":"J. Med. Virol."},{"key":"40077_CR11","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1093\/infdis\/jiu479","volume":"211","author":"KT Franck","year":"2015","unstructured":"Franck, K. T. et al. Sources of calicivirus contamination in foodborne outbreaks in Denmark, 2005\u20132011-the role of the asymptomatic food handler. J. Infect. Dis. 211, 563\u201370 (2015).","journal-title":"J. Infect. Dis."},{"key":"40077_CR12","first-page":"75","volume":"13","author":"B Hebbelstrup Jensen","year":"2016","unstructured":"Hebbelstrup Jensen, B. et al. Enteroaggregative Escherichia coli in Daycare\u2014A 1-Year Dynamic Cohort Study. Front. Cell Infect. Microbiol. 13, 75 (2016).","journal-title":"Front. Cell Infect. Microbiol."},{"key":"40077_CR13","doi-asserted-by":"publisher","first-page":"e89496","DOI":"10.1371\/journal.pone.0089496","volume":"9","author":"R Enserink","year":"2014","unstructured":"Enserink, R. et al. High detection rates of enteropathogens in asymptomatic children attending day care. PLoS One 9, e89496 (2014).","journal-title":"PLoS One"},{"key":"40077_CR14","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1128\/jcm.22.1.116-118.1985","volume":"22","author":"BL Barr\u00f3n-Romero","year":"1985","unstructured":"Barr\u00f3n-Romero, B. L., Barreda-Gonz\u00e1lez, J., Doval-Ugalde, R., Zerme\u00f1o-Eguia Liz, J. & Huerta-Pe\u00f1a, M. Asymptomatic rotavirus infections in day care centers. J. Clin. Microbiol. 22, 116\u2013118 (1985).","journal-title":"J. Clin. Microbiol."},{"key":"40077_CR15","doi-asserted-by":"publisher","first-page":"e75922","DOI":"10.1371\/journal.pone.0075922","volume":"8","author":"SM Ahmed","year":"2013","unstructured":"Ahmed, S. M., Lopman, B. A. & Levy, K. A Systematic Review and Meta-Analysis of the Global Seasonality of Norovirus. PLoS One 8, e75922 (2013).","journal-title":"PLoS One"},{"key":"40077_CR16","doi-asserted-by":"publisher","first-page":"e134","DOI":"10.1097\/INF.0b013e31827d3b68","volume":"32","author":"MM Patel","year":"2014","unstructured":"Patel, M. M. et al. Global Seasonality of Rotavirus Disease. Pediatr. Infect. Dis. 32, e134\u2013147 (2014).","journal-title":"Pediatr. Infect. Dis."},{"key":"40077_CR17","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1017\/S0950268813000435","volume":"142","author":"H Bernard","year":"2014","unstructured":"Bernard, H., H\u00f6hne, M., Niendorf, S., Altmann, D. & Stark, K. Epidemiology of norovirus gastroenteritis in Germany 2001\u20132009: eight seasons of routine surveillance. Epidemiol. Infect. 142, 63\u201367 (2014).","journal-title":"Epidemiol. Infect."},{"key":"40077_CR18","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1111\/j.1651-2227.1999.tb00998.x","volume":"88","author":"AJ Barros","year":"1999","unstructured":"Barros, A. J., Ross, D. A., Fonseca, W. V., Williams, L. A. & Moreira-Filho, D. C. Preventing acute respiratory infections and diarrhoea in child care centres. Acta Paediatr. 88, 1113\u20131118 (1999).","journal-title":"Acta Paediatr."},{"key":"40077_CR19","doi-asserted-by":"publisher","first-page":"1672","DOI":"10.1111\/j.1651-2227.2008.01057.x","volume":"97","author":"A Lennell","year":"2008","unstructured":"Lennell, A. et al. Alcohol-based hand-disinfection reduced children\u2019s absence from Swedish day care centers. Acta Paediatr. 97, 1672\u20131680 (2008).","journal-title":"Acta Paediatr."},{"key":"40077_CR20","doi-asserted-by":"publisher","first-page":"E78","DOI":"10.1542\/peds.108.5.e78","volume":"108","author":"LV Venczel","year":"2001","unstructured":"Venczel, L. V. et al. The role of child care in a community-wide outbreak of hepatitis A. Pediatrics 108, E78 (2001).","journal-title":"Pediatrics"},{"key":"40077_CR21","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1111\/apa.13209","volume":"105","author":"B Hebbelstrup Jensen","year":"2016","unstructured":"Hebbelstrup Jensen, B. et al. Childhood diarrhoea in Danish day care centres could be associated with infant colic, low birthweight and antibiotics. Acta Paediatr 105, 90\u201395 (2016).","journal-title":"Acta Paediatr"},{"key":"40077_CR22","doi-asserted-by":"publisher","first-page":"1707","DOI":"10.1128\/JCM.00037-17","volume":"55","author":"P Jokelainen","year":"2017","unstructured":"Jokelainen, P. et al. Dientamoeba fragilis, a Commensal in Children in Danish Day Care Centers. J. Clin. Microbiol. 55, 1707\u20131713 (2017).","journal-title":"J. Clin. Microbiol."},{"key":"40077_CR23","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1111\/apa.13239","volume":"105","author":"M S\u00f6derstr\u00f6m","year":"2016","unstructured":"S\u00f6derstr\u00f6m, M. Diarrhoea risk study underlines the difficulties in carrying out research in day care centre settings. Acta Paediatr. 105, 13\u201314 (2016).","journal-title":"Acta Paediatr."},{"key":"40077_CR24","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1016\/j.jcv.2008.08.001","volume":"43","author":"CD Kirkwood","year":"2008","unstructured":"Kirkwood, C. D. & Streitberg, R. Calicivirus shedding in children after recovery from diarrhoeal disease. J. Clin. Virol. 43, 346\u2013348 (2008).","journal-title":"J. Clin. Virol."},{"key":"40077_CR25","doi-asserted-by":"publisher","first-page":"962","DOI":"10.1097\/01.inf.0000183748.41027.a4","volume":"24","author":"V Rosenfeldt","year":"2005","unstructured":"Rosenfeldt, V. et al. Viral etiology and incidence of acute gastroenteritis in young children attending day-care centers. Pediatr. Infect. Dis. J. 24, 962\u2013965 (2005).","journal-title":"Pediatr. Infect. Dis. J."},{"key":"40077_CR26","unstructured":"Vaccination against rotavirus \u2013 a medical technological assessment. The Danish Health Authorities (Sundhedsstyrelsen). http:\/\/www.sst.dk\/mtv (2012)."},{"key":"40077_CR27","doi-asserted-by":"publisher","first-page":"1661","DOI":"10.1002\/jmv.23641","volume":"85","author":"I Mukhopadhya","year":"2013","unstructured":"Mukhopadhya, I. et al. Rotavirus shedding in symptomatic and asymptomatic children using reverse transcription-quantitative PCR. J. Med. Virol. 85, 1661\u20131668 (2013).","journal-title":"J. Med. Virol."},{"key":"40077_CR28","doi-asserted-by":"publisher","first-page":"1755","DOI":"10.1093\/infdis\/jiu361","volume":"210","author":"M Malm","year":"2014","unstructured":"Malm, M., Uusi-Kerttula, H., Vesikari, T. & Blazevic, V. High serum levels of norovirus genotype-specific blocking antibodies correlate with protection from infection in children. J. Infect. Dis. 210, 1755\u20131762 (2014).","journal-title":"J. Infect. Dis."},{"key":"40077_CR29","doi-asserted-by":"publisher","first-page":"3440","DOI":"10.1016\/j.celrep.2018.03.001","volume":"22","author":"LB Thackray","year":"2018","unstructured":"Thackray, L. B. et al. Oral Antibiotic Treatment of Mice Exacerbates the Disease Severity of Multiple Flavivirus Infections. Cell Rep. 22, 3440\u20133453.e6 (2018).","journal-title":"Cell Rep."},{"key":"40077_CR30","doi-asserted-by":"publisher","first-page":"1710","DOI":"10.1017\/S095026881400274X","volume":"143","author":"PF Teunis","year":"2015","unstructured":"Teunis, P. F. et al. Shedding of norovirus in symptomatic and asymptomatic infections. Epidemiol. Infect. 143, 1710\u20131717 (2015).","journal-title":"Epidemiol. Infect."},{"key":"40077_CR31","doi-asserted-by":"publisher","first-page":"516","DOI":"10.1016\/S0022-3476(05)82477-1","volume":"120","author":"R Van","year":"1992","unstructured":"Van, R. et al. Outbreaks of human enteric adenovirus types 40 and 41 in Houston day care centers. J. Pediatr. 120, 516\u2013521 (1992).","journal-title":"J. Pediatr."},{"key":"40077_CR32","doi-asserted-by":"crossref","first-page":"438","DOI":"10.7883\/yoken.JJID.2008.438","volume":"61","author":"S Usuku","year":"2008","unstructured":"Usuku, S., Kumazaki, M., Kitamura, K., Tochikubo, O. & Noguchi, Y. An outbreak of food-borne gastroenteritis due to sapovirus among junior high school students. Jpn. J. Infect. Dis. 61, 438\u2013441 (2008).","journal-title":"Jpn. J. Infect. Dis."},{"key":"40077_CR33","doi-asserted-by":"publisher","first-page":"667","DOI":"10.1093\/infdis\/149.5.667","volume":"149","author":"H Champsaur","year":"1984","unstructured":"Champsaur, H. et al. Rotavirus carriage, asymptomatic infection, and disease in the first two years of life. I. Virus shedding. J. Infect. Dis. 149, 667\u2013674 (1984).","journal-title":"J. Infect. Dis."},{"key":"40077_CR34","doi-asserted-by":"publisher","first-page":"3427","DOI":"10.1128\/JCM.01589-12","volume":"50","author":"B Kapusinszky","year":"2012","unstructured":"Kapusinszky, B., Minor, P. & Delwart, E. Nearly constant shedding of diverse enteric viruses by two healthy infants. J. Clin. Microbiol. 50, 3427\u20133434 (2012).","journal-title":"J. Clin. Microbiol."},{"key":"40077_CR35","doi-asserted-by":"publisher","first-page":"Suppl, S288","DOI":"10.1086\/315590","volume":"181","author":"XL Pang","year":"2000","unstructured":"Pang, X. L., Honma, S., Nakata, S. & Vesikari, T. Human caliciviruses in acute gastroenteritis of young children in the community. J. Infect. Dis. 181, Suppl, S288\u201394 (2000).","journal-title":"J. Infect. Dis."},{"key":"40077_CR36","doi-asserted-by":"publisher","first-page":"958","DOI":"10.1017\/S0950268812001586","volume":"141","author":"SK Dey","year":"2013","unstructured":"Dey, S. K., Hoq, I., Okitsu, S., Hayakawa, S. & Ushijima, H. Prevalence, seasonality, and peak age of infection of enteric adenoviruses in Japan, 1995\u20132009. Epidemiol. Infect. 141, 958\u2013960 (2013).","journal-title":"Epidemiol. Infect."},{"key":"40077_CR37","doi-asserted-by":"crossref","first-page":"2312","DOI":"10.1128\/JCM.37.7.2312-2316.1999","volume":"37","author":"M Blom","year":"1999","unstructured":"Blom, M., Meyer, A., Gerner-Smidt, P., Gaarslev, K. & Espersen, F. Evaluation of Statens Serum Institut enteric medium for detection of enteric pathogens. J. Clin. Microbiol. 37, 2312\u20132316 (1999).","journal-title":"J. Clin. Microbiol."},{"key":"40077_CR38","doi-asserted-by":"publisher","first-page":"1220","DOI":"10.1128\/JCM.42.3.1220-1223.2004","volume":"42","author":"JJ Verweij","year":"2004","unstructured":"Verweij, J. J. et al. Simultaneous detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in fecal samples by using multiplex real-time PCR. J. Clin. Microbiol. 42, 1220\u20131223 (2004).","journal-title":"J. Clin. Microbiol."},{"key":"40077_CR39","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1016\/j.mcp.2007.05.006","volume":"21","author":"JJ Verweij","year":"2007","unstructured":"Verweij, J. J. et al. Real-time PCR for the detection of Dientamoeba fragilis in fecal samples. Mol. Cell Probes 21, 400\u2013404 (2007).","journal-title":"Mol. Cell Probes"},{"key":"40077_CR40","doi-asserted-by":"publisher","first-page":"3189","DOI":"10.1128\/JCM.00915-06","volume":"44","author":"C Logan","year":"2006","unstructured":"Logan, C., O\u2019Leary, J. J. & O\u2019Sullivan, N. Real-time reverse transcription-PCR for detection of rotavirus and adenovirus as causative agents of acute viral gastroenteritis in children. J. Clin. Microbiol. 44, 3189\u20133195 (2006).","journal-title":"J. Clin. Microbiol."},{"key":"40077_CR41","doi-asserted-by":"publisher","first-page":"1347","DOI":"10.1002\/jmv.20699","volume":"78","author":"T Oka","year":"2006","unstructured":"Oka, T. et al. Detection of human sapovirus by real-time reverse transcription-polymerase chain reaction. J. Med. Virol. 78, 1347\u20131353 (2006).","journal-title":"J. Med. Virol."},{"key":"40077_CR42","doi-asserted-by":"publisher","first-page":"516","DOI":"10.1111\/j.1469-0691.2007.01692.x","volume":"13","author":"S Persson","year":"2007","unstructured":"Persson, S., Olsen, K. E. P., Scheutz, F., Krogfelt, K. A. & Gerner-Smidt, P. A method for fast and simple detection of major diarrhoeagenic Escherichia coli in the routine diagnostic laboratory. Clin. Microbiol. Infect. 13, 516\u2013524 (2007).","journal-title":"Clin. Microbiol. Infect."},{"key":"40077_CR43","doi-asserted-by":"publisher","first-page":"1057","DOI":"10.1111\/j.1469-0691.2008.02092.x","volume":"14","author":"S Persson","year":"2008","unstructured":"Persson, S., Torpdahl, M. & Olsen, K. E. P. New multiplex PCR method for the detection of Clostridium difficile toxin A (tcdA) and toxin B (tcdB) and the binary toxin (cdtA\/cdtB) genes applied to a Danish strain collection. Clin. Microbiol. Infect. 14, 1057\u20131064 (2008).","journal-title":"Clin. Microbiol. Infect."},{"key":"40077_CR44","doi-asserted-by":"publisher","first-page":"2020","DOI":"10.1128\/JCM.02591-06","volume":"45","author":"S Persson","year":"2007","unstructured":"Persson, S., Olsen, K. E. P., Ethelberg, S. & Scheutz, F. Subtyping method for Escherichia coli shiga toxin (verocytotoxin) 2 variants and correlations to clinical manifestations. J. Clin. Microbiol. 45, 2020\u20132024 (2007).","journal-title":"J. Clin. Microbiol."},{"key":"40077_CR45","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.jcv.2010.07.019","volume":"49","author":"NM van Maarseveen","year":"2010","unstructured":"van Maarseveen, N. M., Wessels, E., de Brouwer, C. S., Vossen, A. C. T. M. & Claas, E. C. J. Diagnosis of viral gastroenteritis by simultaneous detection of Adenovirus group F, Astrovirus, Rotavirus group A, Norovirus genogroups I and II, and Sapovirus in two internally controlled multiplex real-time PCR assays. J. Clin. Virol. 49, 205\u2013210 (2010).","journal-title":"J. Clin. Virol."},{"key":"40077_CR46","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1128\/JCM.06012-11","volume":"50","author":"CR Stensvold","year":"2012","unstructured":"Stensvold, C. R. & Nielsen, H. V. Comparison of microscopy and PCR for detection of intestinal parasites in Danish patients supports an incentive for molecular screening platforms. J. Clin. Microbiol. 50, 540\u2013541 (2012).","journal-title":"J. Clin. Microbiol."},{"key":"40077_CR47","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-7-50","volume":"7","author":"H Mirsepasi","year":"2014","unstructured":"Mirsepasi, H. et al. Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction. BMC Res. Notes 7, 50 (2014).","journal-title":"BMC Res. Notes"},{"key":"40077_CR48","unstructured":"Dean, A. G., Sullivan, K. M. & Soe, M. M. OpenEpi: Open Source Epidemiologic Statistics for Public Health, Version 3.01. www.OpenEpi.com (2018)."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-019-40077-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-019-40077-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-019-40077-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T02:57:29Z","timestamp":1694660249000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-019-40077-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,1]]},"references-count":48,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,12]]}},"alternative-id":["40077"],"URL":"https:\/\/doi.org\/10.1038\/s41598-019-40077-9","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,1]]},"assertion":[{"value":"2 October 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 January 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 March 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"3286"}}