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The absence until recently of genetic tools is the main cause of this gap. We discovered three new potential DNA-associated proteins of <jats:italic>Waddlia chondrophila<\/jats:italic>, a <jats:italic>Chlamydia<\/jats:italic>-related bacterium, using heparin chromatography coupled to mass spectrometry (Wcw_0377, Wcw_1456, and Wcw_1460). By ChIP-seq analysis, we determined the regulatory landscape of these three proteins and we showed that Wcw_0377 binds all along the genome whereas Wcw_1456 and _1460 possess a wide regulon with a large number of co-regulated genes. Wcw_1456 and Wcw_1460 interact with RpoD (\u03c3<jats:sup>66<\/jats:sup>), emerging as potential RpoD regulators. On the other hand, Wcw_0377 is able to reach the host nucleus, where it might interact with eukaryotic histones through its putative chromatin-remodelling SWIB\/MDM2 domain.<\/jats:p>","DOI":"10.1038\/s41598-019-40732-1","type":"journal-article","created":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T11:03:45Z","timestamp":1553079825000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Identification of new DNA-associated proteins from Waddlia chondrophila"],"prefix":"10.1038","volume":"9","author":[{"given":"Marie","family":"de Barsy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas","family":"Herrgott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5725-7929","authenticated-orcid":false,"given":"Trestan","family":"Pillonel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick H.","family":"Viollier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9529-3317","authenticated-orcid":false,"given":"Gilbert","family":"Greub","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,3,20]]},"reference":[{"key":"40732_CR1","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1038\/nrmicro.2016.103","volume":"14","author":"DF Browning","year":"2016","unstructured":"Browning, D. 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