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However, the extent to which it impacts malaria transmission remains unclear. Here, we investigated the association between a marker of glutathione S-transferase mediated metabolic resistance and <jats:italic>Plasmodium<\/jats:italic> infection in field population of <jats:italic>Anopheles funestus<\/jats:italic> s.s. in comparison to the A296S-RDL target site mutation. The 119F-GSTe2 resistant allele was present in southern (Obout) (56%) and central (Mibellon) (25%) regions of Cameroon whereas the 296S-RDL resistant allele was detected at 98.5% and 15% respectively. The whole mosquito <jats:italic>Plasmodium<\/jats:italic> and sporozoite infection rates were 57% and 14.8% respectively in Obout (n\u2009=\u2009508) and 19.7% and 5% in Mibellon (n\u2009=\u2009360). No association was found between L119F-GSTe2 genotypes and whole mosquito infection status. However, when analyzing oocyst and sporozoite infection rates separately, the resistant homozygote 119F\/F genotype was significantly more associated with <jats:italic>Plasmodium<\/jats:italic> infection in Obout than both heterozygote (OR\u2009=\u20092.5; P\u2009=\u20090.012) and homozygote susceptible (L\/L119) genotypes (OR\u2009=\u20092.10; P\u2009=\u20090.013). In contrast, homozygote RDL susceptible mosquitoes (A\/A296) were associated more frequently with <jats:italic>Plasmodium<\/jats:italic> infection than other genotypes (OR\u2009=\u20094; P\u2009=\u20090.03). No additive interaction was found between L119F and A296S. Sequencing of the <jats:italic>GSTe2<\/jats:italic> gene showed no association between the polymorphism of this gene and <jats:italic>Plasmodium<\/jats:italic> infection. Glutathione S-transferase metabolic resistance is potentially increasing the vectorial capacity of resistant <jats:italic>An<\/jats:italic>. <jats:italic>funestus<\/jats:italic> mosquitoes. This could result in a possible exacerbation of malaria transmission in areas of high GSTe2-based metabolic resistance to insecticides.<\/jats:p>","DOI":"10.1038\/s41598-019-42015-1","type":"journal-article","created":{"date-parts":[[2019,4,8]],"date-time":"2019-04-08T10:04:10Z","timestamp":1554717850000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":74,"title":["A marker of glutathione S-transferase-mediated resistance to insecticides is associated with higher Plasmodium infection in the African malaria vector Anopheles funestus"],"prefix":"10.1038","volume":"9","author":[{"given":"Magellan","family":"Tchouakui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mu-Chun","family":"Chiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cyrille","family":"Ndo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carine K.","family":"Kuicheu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8097-6630","authenticated-orcid":false,"given":"Nathalie","family":"Amvongo-Adjia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murielle J.","family":"Wondji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micareme","family":"Tchoupo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael O.","family":"Kusimo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob M.","family":"Riveron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0791-3673","authenticated-orcid":false,"given":"Charles S.","family":"Wondji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,4,8]]},"reference":[{"key":"42015_CR1","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1038\/nature15535","volume":"526","author":"S Bhatt","year":"2015","unstructured":"Bhatt, S. et al. 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