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Quantitative reverse transcription PCR (RT-qPCR) is a reliable and reproducible technique for measuring changes in target gene expression across various biological conditions. A crucial aspect of accurate normalization is the choice of appropriate internal controls. To identify reference genes for accurate evaluation of expression levels in <jats:italic>T. interdigitale<\/jats:italic>, the transcription levels of eight candidate reference genes (<jats:italic>adp-rf, \u03b2-act<\/jats:italic>, <jats:italic>ef1-\u03b1<\/jats:italic>, <jats:italic>gapdh<\/jats:italic>, <jats:italic>psm1, sdha<\/jats:italic>, <jats:italic>rpl2<\/jats:italic> and <jats:italic>ubc)<\/jats:italic> and one target gene (<jats:italic>Tri m4<\/jats:italic>) were analysed by RT-qPCR after growing the dermatophyte under different environmental conditions. The results obtained from expression stability evaluations with NormFinder, geNorm, BestKeeper, and RefFinder software demonstrated that <jats:italic>adp-rf<\/jats:italic> and <jats:italic>psm1<\/jats:italic> were the most stable internal control genes across all experimental conditions. The present study constitutes the first report of the identification and validation of reference genes for RT-qPCR normalization for <jats:italic>T. interdigitale<\/jats:italic> grown under different environmental conditions resembling the conditions encountered by fungi during invasion of skin.<\/jats:p>","DOI":"10.1038\/s41598-019-42065-5","type":"journal-article","created":{"date-parts":[[2019,4,3]],"date-time":"2019-04-03T10:17:37Z","timestamp":1554286657000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Reference genes for accurate evaluation of expression levels in Trichophyton interdigitale grown under different carbon sources, pH levels and phosphate levels"],"prefix":"10.1038","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0172-0213","authenticated-orcid":false,"given":"Anita","family":"Ciesielska","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beata","family":"Oleksak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pawe\u0142","family":"St\u0105czek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,4,3]]},"reference":[{"key":"42065_CR1","doi-asserted-by":"crossref","unstructured":"Summerbell, R. 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