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There are few human cellular models to reveal regulatory networks in adipocyte browning and key targets for enhancing thermogenesis in obesity. The Simpson-Golabi-Behmel syndrome (SGBS) preadipocyte line has been a useful tool to study human adipocyte biology. Here we report that SGBS cells, which are comparable to subcutaneous adipose-derived stem cells, carry an FTO risk allele. Upon sustained PPAR\u03b3 stimulation or irisin (a myokine released in response to exercise) treatment, SGBS cells differentiated into beige adipocytes exhibiting multilocular lipid droplets, high UCP1 content with induction of typical browning genes (<jats:italic>Cidea, Elovl3<\/jats:italic>) and the beige marker <jats:italic>Tbx1<\/jats:italic>. The autocrine mediator BMP7 led to moderate browning with the upregulation of the classical brown marker <jats:italic>Zic1<\/jats:italic> instead of <jats:italic>Tbx1<\/jats:italic>. Thermogenesis potential resulted from PPAR\u03b3 stimulation, irisin and BMP7 can be activated in UCP1-dependent and the beige specific, creatine phosphate cycle mediated way. The beige phenotype, maintained under long-term (28 days) conditions, was partially reversed by withdrawal of PPAR\u03b3 ligand. Thus, SGBS cells can serve as a cellular model for both white and sustainable beige adipocyte differentiation and function.<\/jats:p>","DOI":"10.1038\/s41598-019-42256-0","type":"journal-article","created":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T10:03:51Z","timestamp":1554804231000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Differentiating SGBS adipocytes respond to PPAR\u03b3 stimulation, irisin and BMP7 by functional browning and beige characteristics"],"prefix":"10.1038","volume":"9","author":[{"given":"\u00c1gnes","family":"Klus\u00f3czki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zolt\u00e1n","family":"Ver\u00e9b","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Attila","family":"V\u00e1mos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Fischer-Posovszky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Wabitsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zsolt","family":"Bacso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e1szl\u00f3","family":"F\u00e9s\u00fcs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Endre","family":"Krist\u00f3f","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,4,9]]},"reference":[{"key":"42256_CR1","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.gendis.2015.03.001","volume":"2","author":"MD Lynes","year":"2015","unstructured":"Lynes, M. 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